Objective To study the effect and mechanism of Gehua Jiecheng decoction (GHJCD),a tra-ditional Chinese herbal medicine compound,on cell cycle regulation factors-Cyclin D1,CDK4 and p27 KIP1 in HBV transgenic mice with hepatocellular carcinoma (HCC)precancerous lesions induced by alcohol.Methods Altogether 24 HBV transgenic mice were randomly divided into normal group,model group and GHJCD group(each n =8).All the mice were sacrificed at the end of the 22 th week,then their livers were dissected.Pathohistological changes of liver were observed by using HE staining,and the expression of CyclinD1,CDK4 and p27 KIP1 protein in hepatic tissue were detected by using immuno-histochemistrical methods.Results Compared with the normal group,the positive expressions of Cy-clinD1 and CDK4 increased and that of p27 KIP1 decreased significantly (P <0.05)in the model group. Compared with the model group,restoration of injured liver tissue was occurred in GHJCD group,and the position expressions of CyclinD1 and CDK4 decreased significantly (P <0.05 )and the expression of p27 KIP1 increased significantly (P <0.05).Conclusion The protective effects of GHJCD on liver injures and HCC precancerous lesions took effects by downregulation of genes of CyclinD1 and CDK4 and upreg-ulation of gene of p27 KIP1 .

Objective To investigate the pharmacological components of "Szechwan Chinaberry Fruit-Rhizoma Corydalis" drug combination and its potential molecular mechanism in the treatment of liver cancer based on network pharmacology. Methods Related databases, such as TCMSP, Uniprot, and GeneCard, were used to obtain the effective components of Szechwan Chinaberry Fruit and Rhizoma Corydalis, their corresponding action targets, and the disease targets of liver cancer, and the intersecting targets of drugs and diseases were selected. In addition, STRING and Metascape databases were used to screen out the core targets of drug action and perform GO function and KEGG pathway enrichment analyses. Results There were 9 active components in Szechwan Chinaberry Fruit and 49 active components in Rhizoma Corydalis, with 1 common component between the two drugs; there were 181 action targets of Szechwan Chinaberry Fruit and 1097 action targets of Rhizoma Corydalis, with 143 common targets between the two drugs. There were 162 intersecting targets between the drug combination and liver cancer, and the main genes involved were IL6, TP53, VEGFA, TNF, and CASP3. KEGG analysis showed that the main pathways involved included cancer pathway, AGE-RAGE signaling pathway of diabetes complications, TNF signaling pathway, NF-κB signaling pathway, and thyroid hormone signaling pathway. Conclusion There are many different components in the drug combination of "Szechwan Chinaberry Fruit-Rhizoma Corydalis", which can exert a therapeutic effect on liver cancer by acting on related genes and signaling pathways.