1.The Progress of Study on Cell Mechanism of Lead Neurotoxicity
Journal of Environment and Health 1992;0(02):-
The most severe adverse effect of lead is neurotoxicity. The brain in development is extremely sensitive to lead. The present paper reviewed the research advances in cell mechanisms of lead neurotoxicity achieved in recent years , namely the competition effect with calcium ion, the impact on neurotransmitter, the lipid peroxidation damage on hippocampus neuron.
3.Research status of the extraction methods, biological activites and application of yeast β-glucan
Chinese Journal of Biochemical Pharmaceutics 2017;37(3):15-19
By searching and analizing the related literatures of recent years, the extraction methods, biological activites and application of yeastβ-glucan were reviewed.It is found that there are many kinds of extraction methods, including acid method, alkali method, acid-alkali method, alkali-enzymatic method, autolysis-enzyme-alkali method, induced autolysis-alkali method, microwave assisted alkali-enzyme method, autolysis-ultrasonic coupling method, enzymatic method, etc.There are lots of biological activity study report on yeast β-glucan, envolving the activities of immune, anti-tumor, anti-oxidant, anti-radiation, lowering blood lipid, and regulating intestinal flora as prebiotics, etc.Yeast β-glucan is widely used in animal breeding industry, food industry and cosmetics industry.From the review we can know that seeking efficient, fast, pollution-free yeastβ-glucan extraction methods is the hot research point, and it will have more applications in food and cosmetics industries as good biological response regulator in the future .
4.Advances of DNA extraction methods
Chinese Journal of Marine Drugs 1994;0(02):-
DNA extraction is a basic technology of molecular biology. The purity and the integrality of DNA structure are necessary for different experiments of gene engineering. In recent years there have been some new or improved DNA extraction methods appeared. The methods of DNA extraction from animals, plants, microorganisms and marine organisms were summarized in this article.
5.A longitudinal study of evaluating the quality of life of esophageal carcinoma patients
Fengshan AN ; Jinqiu HUANG ; Shaohu CHEN ;
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(03):-
Objective: To evaluate the impact of esophagectomy and palliative treatment on short term and long term quality of life (QL) in patients with esophageal cancer. Methods: 88 consective patients treated with radical esophagectomy or palliative treatment were scored by European organization of cancer research and treatment (EORTC), QLQ C30 measurement of QL before treatment and at regular intervals until death or 3 years after operation. The cases were divided into 3 groups and mean scores were obtained from each group. Patients surviving more than 2 years were classified as group 1, with group 2 surviving less than 2 years, and group 3 undergoing palliative treatment. Results: Baseline functional and symptomatic QL scores were similar before treatment in the first and second group, which were higher than scores from the third group. Six weeks after esophagectomy, patients reported worse functional, symptom and global QL scores than before treatment. In the first group, QL scores returned to preoperative level within 9 months. However, improved symptom of dysphagia the second group never regained their former QL scores apart from dysphagia improved after surgery. QL scores of the third group were similar to those of the second group after treatment. Conclusion: Esophagectomy might have a negative impact on patients QL. This effect was found to be transient in the first group but inreversible in the second group. It might seem reasonable for patients in late stage to be treated palliatively.
6.Preparation of pingyangmycin water-soluble wax stick and the content determination
Juan LIU ; Jun YAN ; Fengshan WANG
Chinese Journal of Biochemical Pharmaceutics 2014;(1):82-84
Objiective To prepare pingyangmycin water-soluble wax stick and to establish its content determination method. Method The formula was optimized by orthogonal experiment and the content of pingyangmycin in wax stick was determined by high performance liquid chromatography (HPLC). Results The formula of pingyangmycin wax stick matrix was optimized as 1 g of alcohol ethoxylate and 1 g of S 40. Pingyangmycin was added when the temperature of the matrix raised to 70℃and stirred for 20 min. The linear range of pingyangmycin determined by HPLC was 34.4~172μg/mL,the regression equations was y=8298.9 x-34996(r=0.9999),and the average recovery of pingyangmycin was 102.67%(n=9). Conclusion The Preparing procedure of pingyangmycin water-soluble wax sticks is simple and stable. The HPLC method for determining the content of pingyangmycin in water-soluble wax sticks is simple, fast and accurate.
7.Cheiloplasty by incising along the outline for correction of the defection caused by cheiloschisis operation
Jinling LU ; Jiying DONG ; Fengshan GAO
Chinese Journal of Medical Aesthetics and Cosmetology 2002;0(02):-
Objective To find a satisfied repairing method which can complete the functional cheiloplasty while avoiding the visible scar after the cheiloschisis II operation for bilateral cleft lip. Methods The functional operation was carried out in subcutaneous layer by incising along the outline of the nose and the mouth. Results A satisfied effect was achieved after 6 months to 2 years' follow up in 15 cases of the patients (4 cases of bilateral cleft, 11 cases of cheiloschisis). Conclusions The new operation method has improved the traditional method of cheiloplasty. It can effectively repair the secondary defection in nose and mouth resulted from the cheilochisis operation. It can rebuild the orbicularis oris muscle and repair the diseased alar catilage as well. Furthermore, it not only brings a natural looking appearance, but also a natural function and an invisible incision scar. [
8.Treatment of recurrent cavernous hemangiomas by ethanol thrombosis and pinyangmycin sclerothery
Fengshan GAO ; Jiying DONG ; Jun DU
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(06):-
Objective To investigate a safe, effective and mini-invasive method in the treatment of recurrent cavernous hemangiomas. Methods Under local or ketamine anesthesia, ethanol was injected into hemangioma to embolize the draining vessels, and 8~16 mg pinyangmycin was then injected into the lesion slowly. After 7~10 days, second injection of pinyangmycin and total 3~5 times were needed as a course of treatment. Results In the 6 cases reported, shrinkage and degradation were observed and finally tumor was disappeared. There was no skin necrosis, no recurrence and other complications in the 1~5 years follow-up period. Conclusion Ethanol embolization and pinyangmycin injection is a safe, simple, mini-invasive, and effective method in treating the recurrent cavernous hemangiomas.
9.Introduction of limb reservation operation for an infected and all muscles and intermuscular vessels necrosed leg
Fengshan HAN ; Guangnan WANG ; Juan PING
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective to study the factors of delaying survival of the ankle and foot which were linked with leg only by skin, fascia and tibia after all the infected and inactivated muscles and intermuscular vessels had been removed, and to study the effect of artery blood supply reconstruction to avoid the necrosis and ischemia contracture of the foot. Methods The leg was linked with ankle and foot by the tibia which was covered only by skin and fascia after all the muscles, interosseous membrance, intermuscular vessels and fibula had been removed. And then the artery blood supply for the injured limb was reconstructed by stages. Results The sense of haphalgesia, with normal temperature and color, recovered in the injured limb 7 months after operation, no contracture and deformity happened in the food muscles and joint, and the patient may smooth stand and walk with a stick freely. Conclusion The necrosis and ischemia contracture of foot was delayed by reticular collateral circulation among skin, deep and superficial fascia and tibia of the injured limb, although the blood supply was very little after all the muscles and intermuscular vessels had been removed because of infection and necrosis. Finally the injured limb was reserved successfully by artery blood supply reconstruction. The clinical effect was satisfactory.
10.The treatment of reconstructing long bone defects as a result of longterm postoperative infections following fractures in the childhood
Fengshan HAN ; Hongwei ZHANG ; Gaoshan LI
Medical Journal of Chinese People's Liberation Army 2001;0(12):-
Objective To probe the pathogenesis of long bone defects and damage to epiphysis as the result of postoperative infections following fractures in the childhood of patients, and to look for ideal ways to prevent and repair the defects and damages. Methods The treatment of the infected bone defect of a tibia had been continued for one year following fracture in one four-year old patient, and the treatment of the infected bone defect of a humerus had been continued for ten years following fracture in another seven-year old patient, but all the treatments had failed. The injured limbs developed bone infection, bone defect, bone exposure, shortening, and pseudoarthrosis deformities at last. Then the lesions were completely cleaned, and the deformities of the infected bones were corrected in one stage, with reconstruction of the bone and the repair of the wounds by transplantations of fibular free flaps. Results Both fibular free flaps survived in one stage transplantation. The bones of the injured limbs healed half a year after the operations, and weight-bearing and walking functions of the injured limbs recovered as well. Conclusion Transplantation of fibular free flap was an ideal way to treat a long bone defect as a result of postoperative infection following fracture in the childhood. The early appropriate treatment of postoperative infection following fracture is very important to avoid bone and epiphysis damages.