Objective:To study the efficacy and safety of different types of quinolone antibiotics in the treatment of pelvic inflamma -tory diseases .Methods:Totally 70 patients with pelvic inflammatory diseases were selected and divided into the observation group and the control group according to the time of admission .The 35 patients in the observation group were treated with levofloxacin , and the con-trol group was treated with ciprofloxacin .After 20-day treatment, the clinical efficacy was observed and recorded in the two groups , and the adverse events were statistically analyzed .Results:The total effective rate of the observation group was 97.14%, which was signifi-cantly higher than that of the control group (82.86%, P<0.05).The recovery time of clinical symptoms and signs of the observation group was significantly shorter than that of the control group , and the difference was statistically significant (P<0.05).The difference in the incidence of adverse drug reactions was not statistically significant between the two groups (P>0.05).Conclusion:Levofloxacin in the treatment of patients with pelvic inflammatory diseates is with high clinical efficacy , which can rapidly improve the clinical symptoms and signs with high security .

[Objective]To investigate the inhibitory effect and mechanism of the microRNA-320d(miR-320d)on epithelial mesenchymal transition in endometrial carcinoma JEC cells.[Methods]JEC endometrial carcinoma cell lines were transfected with miR-320d mimics or negative control mimic,respectively,as M320d or NCM group. Control group was established with untreated JEC endometrial carcinoma cells. miR-320d content in each group was detected by RT-PCR method. Transwell assay was used to detect the migration and invasion ability of the 3 groups. Western-blot assay was used to detect the expressions ofα-Catenin,E-cad-herin,Vimentin and PBX3 protein in 3 groups. Antagonistic effect of PBX3 overexpression on miR-320d inhibition of EMT was detect-ed by western blot assay. The relationship between miR-320d and PBX3 was detected by dual luciferase assay.[Results]The expres-sion level of miR-320d in M320d group was significantly up-regulated,and the expression level of miR-320d was 808.25 ± 15.58 times higher than that of control group(P<0.05). The number of migrating cells in M320d group was 29.56 ± 0.59,which was signif-icantly lower than that of control group at 94.48 ± 1.02(P < 0.05). The number of invasive cells in M320d group was 7.33 ± 0.84, which was significantly lower than that of group control 86.28 ± 3.51(P < 0.05). Compared with control group ,the expression of α-Catenin and E-cadherin protein was significantly increased ,the expression of Vimentin protein was significantly decreased ,and the expression of PBX3 protein was significantly decreased. After PBX3 overexpression,the expression ofα-Catenin and E-cadherin protein were significantly decreased,the expression of Vimentin protein were significantly increased. Dual luciferase assay showed that PBX3 is a downstream target gene of miR-320d(P<0.05).[Conclusion]miR-320d may inhibit the expression of EMT related protein through the downstream target gene PBX3 and inhibit the epithelial mesenchymal transition function of endometrial carcinoma JEC cells.