Objective To study the efficacy of arthroscopy in the management of patella fracture.Methods Arthroscopic techniques were employed for treating 42 patients with fresh simple patella fracture.After blood clots and fragments were cleaned up,the fracture was reduced by manipulation.Two Kirschner's pins were parallelly inserted percutaneously for internal fixation of the fracture.Then a modified tension band fixation with percutaneous wire interposition was made.Results The operation time was(1.2~3) hours(mean,2.2 hours).The time to bone union was 7~10 weeks(mean,7.5 weeks).The postoperative hospital stay was 7~14 days(mean,8.5 days).Follow-up checkups in 37 patients for 6 months ~ 4 years(mean,19.2 months) revealed bone union in all the patients and no joint instability or traumatic arthritis.The curative results were classified as excellent in 34 patients,good in 2 patients,and fair in 1,the rate of excellent or good results being 97.3%(36/37).Conclusions Arthroscopic management of patella fracture has advantages of accurate reduction and fixation,little invasion,early postoperative functional training,and quick recovery.

OBJECTIVE: To develop an HPLC method for the determination of salvianolic acid B in Qianlie kangxin granules.METHODS: The sample was determined on DiamonsilTM C18(250 mm?4.6 mm,5 ?m) with the mobile phase consisted of methanol-acetonitrile-water-formic acid(25∶10∶64∶1) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 286 nm.RESULTS:The linear range of salvianolic acid B was 0.206～1.854 ?g(r=0.999 9).The average recovery rate for salvianolic acid B was 98.0%(RSD=1.5%,n=6).CONCLUSION: The established method is simple,reliable,stable in results and reproducible,and it is applicable for the quality control of Qianlie kangxin granules.

Objective To provide scientific basis for the investigation, prevention,control and administration of house decoration noise Methods The equivalent noise levels (Leq),and the levels of noise dose (D L) in decorated houses,next door houses and outdoor environment were measured during different periods such as morning,noon,afternoon and evening in one day, as well as the health effects of noise in 3 residential quarters were investigated Results The Leqs levels during per 10 minutes were 79 6～104 7 dB(A) in decorated house The individuals exposure D L of decoration workers were 90%～180% The Leqs during per 20 minutes were 70 9～76 4 dB(A) in next door houses and 63 4～76 5dB(A) in the neiboaring residential building and office building 6～45 meters far from the decoration noise sources The annoyance rate among 149 residents was high and reached 89 8% The mental labourers revealed significantly higher annoyance rate compared with that of physical labourers Conclusion The health effects of decoration noise on occupational individuals and residents shouldnt be ignored

Objective To investigate effects of transcranial direct current stimulation on picture naming by those with Broca's aphasia recovering from a stroke.Methods Five patients with Broca's aphasia 6-12 months post-stroke served as their own controls.They all had lesions of the left frontal gyms or the basal ganglia.They received either anodic, cathodic or sham stimulation of the left Broca's area.They were then given a picture naming test.Results The average number of correct namings was 13.13 with anodic stimulation, 9.80 with cathodic and 10.15 with the sham stimulation.Compared with cathodic or sham stimulation, anodic stimulation of the left Broca's area can improve the picture-naming ability of aphasic stroke patients significantly more effectively.Conclusions Anodic stimulation of the left Broca's area can improve the picture-naming of cerebral apoplexy patients with Broca's aphasia.Such stimulation may increase the excitability of the left cortex, suggesting that the left Broca's area and its periphery play a very important role in language recovery after stroke.

OBJECTIVE: To evaluate the clinical efficacy of Cefepime in treating senile patients with hospital acquired pneumonia(HAP).METHODS: A total of 60 HAP cases were randomly assigned to receive Cefepime(n=30) or Ceftazidime (n=30) for 7～10 days.The effective rate and the bacterial clearance rate of two groups were compared.RESULTS: The effective rate was 86.7% for Cefepime group and 70.0% for Ceftazidime group(P

Aim To study IL-8 expression of human airway epithelium-like NCI-H292 cells directly induced by Pyocyanin and its mechanism through protein kinase C(PKC) and nuclear factor-kappa B(NF-??).Methods ELISA methods were performed to test the expression of IL-8 in NCI-H292 cells infected by Pyocyanin.Western blot was employed to examine the expression of NF-?B protein.In addition,NCI-H292 cells were cultured together with PKC inhibitor,calphostin C or NF-?? inhibitor,pyrrolidine dithiocarbamate(PDTC),respectively before Pyocyanin infection,then the expression of IL-8 and NF-?? was assayed using the above methods.Results Pyocyanin was able to induce IL-8 protein secretion in NCI-H292 cells and had marked concentration-dependent relations.Pyocyanin could also significantly promote the activation of NF-?B,which peaked at 60~90 min.PDTC and calphostin C could significantly decrease the activation of NF-?? and the expression of IL-8.Conclutions Pyocyanin can induce IL-8 production.The direct induction of Pyocyanin can promote the activation of NF-?B by PKC signal pathway,then cause the expression and secretion of IL-8.

To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferation, and phagocytosis were observed. The cells showed no remarkable changes when the energy was low. When the exposure energy reached 1.12 mW/cm2, the cytoplasm showed a rough appearance, and cell proliferation and phagocytosis decreased. This phototoxicity was strong with white light (compound chromatic light), moderate with violet light or yellow light, and mild with red light.
Cell Proliferation/radiation effects ; Cells, Cultured ; *Light ; Phagocytosis/radiation effects ; Trabecular Meshwork/cytology ; Trabecular Meshwork/*radiation effects

Objective:To observe the clinical efficacy of pressing-kneading manipulation combined with herb-insulated moxibustion at Shuidao(ST28)for postpartum urinary retention after labor analgesia and its effect on bladder urination function. Methods:A total of 154 patients with postpartum urinary retention after labor analgesia were randomly divided into a Western medication group and a herb-insulated moxibustion group,with 77 cases in each group.In the Western medication group,neostigmine sulfate was injected into Zusanli(ST36).In the herb-insulated moxibustion group,after pressing-kneading manipulation at Shuidao(ST28),herb-insulated moxibustion was applied to Shuidao(ST28)with self-made Tong Quan San.Both groups were treated once,and the clinical efficacy was evaluated 5 h after treatment.The first urination time,first urination volume,average urinary flow rate,bladder residual urine volume,hospitalization days,and costs were recorded. Results:The total effective rate and markedly effective rate of the herb-insulated moxibustion group were higher than those of the Western medication group(P<0.05),the time to the first urination and residual urine volume in the bladder of the herb-insulated moxibustion group were shorter or smaller than those of the Western medication group(P<0.01),the first urination volume and average urine flow rate of the herb-insulated moxibustion group were larger than those of the Western medication group(P<0.01).There were no significant differences in the hospitalization days and costs between the two groups(P>0.05). Conclusion:Pressing-kneading manipulation combined with herb-insulated moxibustion at Shuidao(ST28)can effectively treat postpartum urinary retention after labor analgesia and improve bladder urination function.

Objective This study was to understand the structure characteristics of prophages in the genome of Enterococcus hirae R17,and also to analyze their interaction relationships with the host bacterium.Methods The gene distribution and gene encoding characteristics of prophages in the genome of Enterococcus hirae R17 were identified using the PHAST software.The virulence gene,antimicrobial resistance genes,and environmental resistance genes in the prophages were also analyzed.Results Three prophages were found on the chromosome of Enterococcus hirae,including two incomplete prophage elements (Prophage-1 and Prophage-2) and one complete prophage (Prophage-3).Some function genes of bacteria were found in the sequence of three prophages,including nucleotide transportation and metabolism related genes.One incomplete prophage carrying erythromycin-and bacitracin-resistance genes was identified in the plasmid,which suggested that prophage induced gene horizontal transfer caused erythromycin-and bacitracin-resistance of Enterococcus hirae R17.Conclusion This study laid a solid foundation for the diversity analysis of prophages of Enterococcus hirae.Prophages played an important role in promotion of antimicrobial resistance of enterococci.Scientists should pay more attention to the spread of antimicrobial resistance and pathogenicity induced by prophages.

Objective To study the application of individualized dressing change on tumor patients with PICC. Methods We made different methods of dressing change in accordance with the status of the patients and the seasons, and observed the color and the integrity of the local skin around the puncture points. Results With the exception of 2 cases requested extubation because of tetter on skin around the puncture point, the local skin infection or systemic infection didn't occur in the other 97 cases by individualized dressing change. Conclusions It can reduce the rate of local infection, extends time of catheter retaining, and improves quality of life of tumnor patients with PICC by individualized dressing change