Objective:To investigate the correlation between heart rate turbulence(HRT)and heart rate variability(HRV)indices,and the impact of mean R-R interval,?-blocker and other factors on HRV and HRT.Methods:76 postinfarction patients were recruited.24-hour,three-lead ambulatory electrocardiography was performed in all subjects for the analysis of HRV(including SDNN,PNN50,RMSSD,HF,LF)and HRT(including TO and TS).And the general clinical data of each case were collected.Results:TS was positively correlated with SDNN(r=0.559,P

Although the evidence-based medicine(EBM) plays an important role in modern medicine,there has been a growing conflict between EBM and the clinical teaching practice in the department of cardiology.By analyzing the reasons,the author has brought forward some suggestions to solve these problems.

Through textual research of processing and related terminology, we provided references for the standard naming of processing and the correct using of related terms. According to the textual research of ancient and modern literature, relationships between processing and related terms were analyzed. The results showed that“Pao”and“Zhi”in ancient times referred to the fire progress of Chinese medicine.“Zhi (Governance)”and“Zhi (making)”meant to change the bias of Chinese medicine through different processing methods.“Processing”contained modern processing methods of firing, watering, cleansing, cutting, and etc. In Han Dynasty,“Pao-Zhi”referred to two kinds of fire methods. However, after the Northern and Southern Dynasties, it referred to many kinds of pharmaceutical technology, such as firing, watering, cleansing, cutting and so on. It had the same concept with processing. Terms such as“Xiu-Shi”,“Xiu-Zhi (Governance)”,“Xiu-Zhi (making)”were also called as“Pao-Zhi (processing)”. It was concluded that processing should be the standardized academic terminology.

0.05). Other raw scores of MMSE in the vascular dementia group were lower than those in the cerebral infarction group and the normal control group ( P 0.05). P 300 peak latency in the vascular dementia group prolonged significantly as compared with that in the cerebral infarction group and the normal control group ( P

Objective To investigate whether taking diastolic potential(DP)and earliest Purkinje potential(PP)as ablation targets together is superior to taking earliest PP alone in patients with left posterior fascicular ventricular tachycardia(VT).MethodsTotally 18 patients who were admitted in our department from May 2006 to May 2009 were enrolled,and the results of their electrophysiological examination were analyzed.According to radiofrequency(RF)ablation targets,patients were classified into 2 groups:DP+PP group(DP and PP as ablation targets together)and PP group(PP as the target alone).Successful RF ablation was established when the ventricular tachycardia was no longer inducible.The acute success rate,recurrent rate,number of ablation application,procedure time,X-ray exposure and complications were compared between DP+PP and PP groups.ResultsThere was 1 case receiving no ablation because of not being induced to clinical VT.Seventeen cases were induced to clinical VT and displayed right bundle branch block(RBBB)and left axis morphology(DP+PP group 7 cases,and PP group 10 cases).All cases were successfully ablated,their DP-Q interval and PP-Q interval were 60.17?8.16 and 30.64?7.19 ms,respectively.There was no significant difference between the 2 groups in the age,ventricular tachycardial cycle,procedure time and recurrent rate.Compared to the PP group,the number of RF application in DP+PP group was fewer(4.55?2.07 vs 7.50?1.64,P=0.04),and X-ray exposure time was a little longer(18.33?1.51 min vs 15.37?2.77 min,P=0.03).There was neither left posterior fascicular block nor other complications seen in the 2 groups.The follow-up period was 14.29?10.05 months,and during this there was 1 case recurrence in DP group(2 months after procedure)and 1 case in PP group(1 month after procedure)respectively.ConclusionThe 2 methods are effective and safe for successful ablation of left posterior fascicular VT.Compared to ablation of earliest PP site alone,ablation of DP+PP might need fewer times of RF application but longer X-ray exposure time.

Objective To study the properties of K~ +-ion channel of rat parietal cells, and to approach its effects on physiological and pathological process. Method Parietal cells were isolated. Electrophysiological records of K~ +-ion channels were made through whole cell's patch clamp technique, and the properties of the channels were analyzed. Results 10mM tetraethylammonium chloride may partially block the outwardly depolarized K~ +-ion channel current. While 10mM tetraethylammonium acetate, 10mM barium chloride or 10mM barium acetate may increases the outwardly depolarized K~ +-ion channel current. Conclusion The whole cell K~ +-ion channel current of rat parietal cells showed voltage sensitive. The current-voltage relations of whole cell potassium channels of rat parietal cells are both outwardly and inwardly rectified.

Objective To investigate the feasibility of transplantation of human fetal liver-delivered mesenchymal stem cells(HFMSCs) and porous microcarries into normal heart tissue and whether it can improve heart function and regeneration of heart tissue.Methods SD rats were divided into HFMSCs injection group(n=9),microcarrier injection group(n=9) and control group(n=4),in which 80-100 ?l Perfadex with HFMSCs or gelatin porous microcarriers or pure Perfadex was injected into the wall of left ventricle.Heart function was evaluated by UCG before and 7 d after transplantation.On day 7,14,the survival of HFMSCs was tested by fluorescent in situ hybridization(FISH),regeneration or cardiac differentiation by immunohistological staining against desmin,tropomyosin and lectin,cellular immune response by the infiltration of macrophages,and lymphocyte reaction to HFMSCs by mixed lymphocyte culture(MLC) in vitro.Results Seven days after injection,the HFMSCs survived and improved the heart function,though no sign of differentiation into cardiomyocytes was seen.On day 14,a large amount of macrophages infiltrated into injection sites,and MLC showed prominent enhancement of proliferation of lymphocytes,when no transplanted cells were detected in the myocardium.On day 7,14,the microcarriers retained their round shape at the injection sites and were attatched by a large quantity of cells which were proven not cardiomyocytes or capilaries by immunohistological staining.Conclusion Transplantation of HFMSCs into normal heart improves heart function by short-period survival without differentiation,but the transplanted cells disappeared because of immune reaction.Transplantation of porous microcarriers into normal heart could not improve heart function either by regeneration of heart tissue or capilaries.

Revised immunoelectron microscopic technique was used to examine G cells and D cells in rat antral mucosa. The number of colloidal gold granules in G and D cells was analysed through quantitative analysis. The results showed that immunological granules of colloidal gold distributed in both G and D cells. Gastrin- or somatostatin-labeled granules were present mainly as lobation-like or island-like congeries. Most of the golden congeries dissociated in the cytoplasm of G or D cells, being in the basement membrane side. A few of the golden congeries located in nucleus. The number of golden granules was 107. 04 ?19. 68 and 83. 36?17. 58 per one G and D cell, respectively.

Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.

Objective To investigate the characteristics of brain imaging and its relationship with the cognitive disorders in vascular dementia patients. Methods The volumes of cerebral lobes and hippocampus formation in 30 ischemic vascular dementia patients and 30 normal controls were measured with MRI based technique. The indices of ventricles and cerebral sulci in 30 ischemic vascular dementia patients and 30 patients with ischemic stroke were calculated with CT based technique. The cognitive functions were assessed by psychometric testing (Mini Mental State Examination MMSE, Brief Screening Scale for Dementia BSSD, Raven's Standard Progress Matric RSPM) in all subjects. Results The volumes of frontal and temporal lobes in vascular dementia patients were smaller than those in ischemic stroke patients and normal control subjects ( P 0.05). The decrease of the volumes of the frontal and temporal lobes were positively correlated with the decrease of MMSE and BSSD scores. The cognitive disorders were more severe in the ischemic vascular dementia patients with pathological foci in the frontal and temporal lobes, multiple infarcts, and the total volume of infarcts greater than 50 mm 3 ( P