OBJECTIVETo evaluate the relationship between osteopontin gene genetic polymorphisms and susceptibility of nasopharyngeal carcinoma in Guangxi Zhuang people.

METHODSWith a hospital based case-control study, osteopontin gene polymorphisms were compared between patients with nasopharyngeal carcinoma and healthy outpatients as a controls in Zhuang population in Guangxi. The single nucleotide polymorphisms at rs1126772 and rs9138 sites of the osteopontin gene were determined by polymerase chain reaction-single base extension technique (PCR-SBE) and DNA sequencing technology. The comparison between genotype and allele frequency distribution differences in case and control group was accomplished by a χ(2) test. The frequencies of haplotypes in osteopontin gene in different groups were analyzed.

RESULTSThere were no differences between the patients and controls in the genotype or allele frequencies of osteopontin gene rs1126772 site (

GA/GGOR = 0.94, 95%CI 0.37-2.37, χ(2) = 0.182, P = 0.891; AA/GG:OR = 0.86, 95%CI 0.35-2.12, χ(2) = 0.834, P = 0.773) or rs9138 site (

CA/CCOR = 1.42, 95%CI 0.88-2.29, χ(2) = 2.023, P = 0.155; AA/CC:OR = 1.77, 95%CI 0.78-4.01, χ(2) = 1.901, P = 0.168). The frequency of GA haplotype in the patients was significantly higher than that in the controls (P = 0.003), and the GA haplotype was associated with a significantly increased risk of nasopharyngeal carcinoma (OR = 4.84, 95%CI 1.59-14.71).

CONCLUSIONThe haplotype GA of osteopontin gene rs1126772 and rs9138 sites increases the risk of nasopharyngeal carcinoma in Guangxi Zhuang people.

Carcinoma ; Case-Control Studies ; China ; Disease Susceptibility ; Gene Frequency ; Genetic Predisposition to Disease ; epidemiology ; Genotype ; Haplotypes ; Humans ; Nasopharyngeal Neoplasms ; epidemiology ; genetics ; Osteopontin ; genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide

Objective To investigate and analyze the incidence of hepatitis B virus (HBV) infection and related cirrhosis in a certain area. Methods A retrospective investigation was performed on 365 patients with HBV infection in a certain area from October 2018 to October 2020. The relevant data of the patients and the incidence of HBV infection-related cirrhosis were analyzed to explore the influencing factors for liver cirrhosis caused by HBV infection. Results The age of patients with HBV infection was mainly 31-50 years old (61.92%), who were mainly males (80.00%). The symptoms included yellow urine (66.30%), loss of appetitte (57.53%) and fatigue (46.85%). There was abnormal increase of aspartate aminotransferase and alanine aminotransferase, and hyperbilirubinemia in patients. 35 patients developed liver failure, of whcih 31 patients survived and were discharged, 3 patients underwent liver transplantation and 1 patient died after discharge. Among the 365 patients, there were 82 cases with HBV-related cirrhosis, mainly aged between 31 and 50 years old (63.41%), who were mainly males (80.00%). The main symptoms included abdominal distension (46.34%), liver palm (39.02%) and jaundice (34.15%), and all were accompanied with abnormal liver function indexes. Of the 365 patients, 35.37% of them were complicated with primary peritonitis, and 25.61% with electrolyte imbalance. In addition, 87.80% of the patients improved and were discharged. The incidence rates of upper gastrointestinal bleeding, hepatic encephalopathy and death were 7.32%, 3.66% and 1.22%, respectively. The results of univariate and multivariate analysis showed that drinking history, HBV-DNA level and exercise were the influencing factors of HBV-related cirrhosis (P<0.05). Conclusion Patients with HBV infection and related cirrhosis are mostly middle-aged men. Drinking history, HBV-DNA level and exercise are important influencing factors for HBV infection progression to cirrhosis.