1.Effects of Abiotic Elicitors MeJA and SA on Alkaloids Accumulation and Related Enzymes Metabolism inPinellia ternata Suspension Cell Cultures
Yongbo DUAN ; Fang LU ; Tingting CUI ; Fenglan ZHAO ; Jingtong TENG ; Wei SHENG ; Aimin ZHANG ; Jianping XUE
Chinese Journal of Information on Traditional Chinese Medicine 2017;24(1):87-90
Objective To study the effects of abiotic elicitors methyl jasmonate (MeJA) and salicylic acid (SA) on the alkaloids accumulation and related enzymes metabolism inPinellia ternata suspension cell cultures. Methods Using the leaf petioles-derived suspension cell cultures as the study object, the culture duration, concentrations of MeJA and SA were determined to get the optimal alkaloids accumulation, and the activities of metabolic enzymes IMP dehydrogenase and sAMP synthase were also measured.Results A 9-fold of dried biomass and a 3-fold of alkaloids accumulation were observed inP. ternata suspension cell cultures after culture for 21 d. Both MeJA and SA could significantly promote the accumulation of alkaloids inP. ternata suspension cells. 150 μmol/L MeJA enhanced alkaloids content (4.7 mg/gDW) by 3.6 folds in comparison with control group, whereas 50 μmol/L SA showed a 2.5-fold increase. Meanwhile, 100 μmol/L MeJA and 50 μmol/L SA promoted the increase in IMP dehydrogenase activity by 3.0 and 3.7 fold respectively, and 150 μmol/L MeJA and 100 μmol/L SA showed the increase by 2.6 and 4.4 fold respectively.Conclusion Proper adding exogenous MeJA and SA can promote the accumulation of alkaloids inPinellia ternata suspension cell cultures.
2.Effect of Early Systematic Health Education on Mother Mood and Baby Intelligence
Bizhu CHEN ; Caixia JIANG ; Fenglan XUE
Journal of Shenyang Medical College 2016;18(5):375-376,384
Objective:To analysis the effect of early systematic health education on mother mood and baby intelligence. Methods:A total of 87 cases of healthy infants were enrolled. According to random number table, they were divided into control group (n=45) who received routine health education,observation group (n=42) who received early systematic health education. Smart baby and mother's emotional state were compared in two groups. Results:After the intervention, compared with control group, anxiety and depression scores in mother was lower, and fine motor, the big movement, adaptability, social behavior and the development of language ability in baby were higher in observation group (P< 0.01) . Conclusion:Early systematic health education to promote infant intelligence development,mother relieve anxiety,depression,mood is of great significance.
3.Effect of Clearing Heat and Resolving Phlegm on Blood Level of Neutrophil Elastase in AECOPD Rats
Jie LI ; Qi WANG ; Cuiling FENG ; Lishan ZHANG ; Zeji QIU ; Jun WU ; Hui LUO ; Fenglan DONG ; Fuding LIU ; Fucheng ZHAO ; Ying QI ; Guangwei XUE
China Pharmacist 2014;(5):722-724
Objective:To preliminarily study the effect of Louqin Zhisou decoctions with the mechanism of clearing heat and resol-ving phlegm on the blood level of neutrophil elastase ( NE) in the rat model of acute exacerbation of chronic obstructive pulmonary dis-ease ( AECOPD) . Methods:The rat model of AECOPD was established by passive cigarette smoking, intratracheal instillation of li-popolysacchricle ( LPY) and intranasal instillation of staphylococcus aureus. Totally 60 AECOPD rats were randomly divided into 3 groups, the model control group (n=20), ambrohexel group (n=20), and Louqin Zhisou decoctions group (n=20). NE was detec-ted by ELISA. Results:Compared with that before the treatment, NE in ambrohexel group and Louqin Zhisou decoctions group was de-creased significantly(P<0. 01). Conclusion:Clearing heat and resolving phlegm method probably can decrease NE by reducing air-way mucus hypersecretion and obstruction in AECOPD rats.