Objective To study the surgical treatment principles of obstructive colorectal cancer. Methods The clinical data of 108 cases of obstructive colorectal cancer treated in our hospital in recent 10 years were retrospectively analysed. Of 30 cases of right hemicolon cancer ,28 cases received primary hemicolectomy and anastomosis,1 underwere conversative operation,and 1 received non-operative treatment.In 78 patients with left hemicolon or rectal cancer,56 underwent primary resection ( primary and secondary anastomosis were performed on 32 and 24 cases, respectively), and 11 received two-stage resection;nonresectable cancer underwent by-pass procedures in 6 cases,and non-operative treatment in 5. Results Most obstructive colorectal cancer were moderate or poor differentiated adenocarcinoma (65 cases) and in Dukes C or D stages(59 cases) . Postoperative complications rate was 27.3%(26 cases,46 times),most were infective complications(35/46) including 4 cases of anastomotic leakage. Eight cases died during perioperative period. The 5-year survavil rate in operative and nonoperative cases was 28.4% and 0% respectively(P0.05). Conclusions The surgical treatment principles for patients with obstructive colorectal cancer is to remove the tumor and relief the obstruction by one-stage operation individually if possible.But one-or two-stage anastomosis after tumor resection seems to be no influence on the patients prognosis.

ObjectiveTo investigate the feasibility of transcranial Doppler (TCD) in evaluating the effect of drugs on reducing the intracranial pressure.Methods20 patients who were treated with 20% mannitol (10 cases) and 10% glycerin (10 cases) separately were monitored the changes of blood flow velocity of middle cerebral artery (MCA) with TCD before and after treatment. ResultsIn mannitol group, The peak time presented at 30 min (P<0.001) after drug application and continued 4～6 h after treatment. In glycerin group, the peak time presented at 2 h (P<0.001) after drug application and continued 6～8 h after treatment.ConclusionTCD can monitor the changes of of blood flow velocity of middle cerebral artery (MCA) and thereby evaluate the effects of drugs on reducing intracranial pressure.

Objective To design a new type of fluorescence detection optical system for dedicated neonatal PKU screening. Methods The system selects super-luminance UVLED for excitation light source, uses different diameter quartz fiber and Y -type optical fiber which consists of glass fiber bundles, and self focusing optical fiber for collimating light and photomultiplier tubes for testing. Results The system has many features, such as simple structure, small size and high sensitivity. Conclusion The clinic screening requirements are met by the system and a new means of rapid detection is provided for PKU screening in large quantities.

Thermal printer is ideal for micro-EGG for its excellences such as high speed, low volume and low noise. Single-lead printing and 6-lead printing are usually adopted. This paper, taking the newly-developed fully automatic ECG based on AduC812 MCU as an example, introduces the realization of different printing manners of ECG and emphasizes the solution of 12-lead fast synchronous printing.

Serum levels of free T3(FT3), free T4(FT4), total T3(TT3), total T4(TT4) and TSHIRMA were measured in 43 and 67 normal subjects and 14 uremic patients, and 21 patients receiving hemodialysis. Significantly lowered mean serum FT4 FT4 and TT3 but normal T4 and TSHIRMA were observed in uremic and hemodialysis ones, although all subjects were in the state of euthyroidism, The rates of low FT3, FT4, TT3 and TT4 were 37.5%, 42.9%, 50% and 21% in uremic patients and 81%, 38.1%, 61.9% and 14.2% in hemodialysis patients, respectively. The postdialysis TSHIRMA level was significantly reduced and FT3, FT4, TT3 and TT4 were unchanged after one hemodialysis. There was no change of the rates of low FT3, FT4 TT3 and TT4 in the course of repeated dialysis.The conclusion is that FT3, FT4, TT3 and TT4 are lowered in most uremic and hemodialysis patients while TSHIRMA is normal or only slightly lowered. The thyroid function of them is most likely normal (euthyroid). TSHIRMA is a useful index in diagnosing hypothyroidism in uremic and hemodialysis patients. Transient and maintained hemodialysis do not dramatically change the thyroid hormones and TSHIRMA levels.

Objective To evaluate the clinical result of modified anterior decompression for the treatment of cervical myelopathy due to ossification of posterior longitudinal ligament(OPLL). Methods From April 1994 to October 2002, 42 cases of OPLL involving 134 cervical segments were operated using modified anterior decompression, among them there were 35 cases, 122 segments of the continuous type; 4 cases, 9 segments of the mixed type; 3 cases, 3 segments of the solitary type. The preoperative index of thickness (O-Index) ranged from 12.4%-78.8%, with an average of 43.2%. A slot was made in the vertebral body through anterior approach and bilateral border of the ossification was dissected from the posterior wall of vertebral body with burr-drill. Results The average follow-up was 3 years and 8 months ranging from 1 year and 6 months to 8 years. The recovery of neurologic function was evaluated according to JOA score: 28 cases with mild neurologic deficit (13-16 points) improved on average 74% postoperatively; 11 cases of the moderately damaged group (8-12 points) improved on 75%; 3 cases of the severely damaged group (

BACKGROUND:Apolipoprotein E is one of the important plasma apolipoproteins with polymorphism and involved in lipid metabolism and cholesterol balance modulation, playing also a role in the normal growth and repair of the nervous system.OBJECTIVE: To analyze apolipoprotein E gene polymorphism in patients with vascular dementia (VaD) in comparison with patients with cerebral infarction (CI) patients and healthy controls, so as to elicit the relationship between apolipoprotein E gene polymorphism and VaD.DESIGN: Case-control study.SETTING: Department of Neurology, General Hospital of Jinan Military Area Command of Chinese PLA.PARTICIPANTS: The participants were recruited from the inpatients and outpatients with cerebrovascular diseases and healthy subjects for routine physical examination in the General Hospital of Jinan Military Area Command of Chinese PLA between August 2001 and October 2003, including 35 cases of VaD, 36 CI cases and 40 healthy controls.METHODS: Four milliliters of venous blood was collected from the elbow vein form all participants after fasting for 12 hours for detection of apolipoprotein E genotype using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Blood lipids and apolipoprotein contents were also examined.of triglyceride, total cholesterol, high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDL-C), as well as apolipoprotein A and aoplipoprotein B.RESULTS: Data of 35 VaD 36 CI patients and 40 healthy controls were all VaD group and CI group had higher ε4 allele frequency were higher (22.86% and 22.22% vs 7.5%, respectively, P ＜ 0.05) but lower ε3 allele frequency (70% and 69.45% vs 86.25%, respectively, P ＜ 0.05); the frequencies of all alleles were basically comparable between VaD and CI groups er in E ε4 allele carriers [(5.85±L03), (4.73±0.29), (4.96±0.87) mmol/L, respectively] than in E ε2 [(3.91±0.87), (3.12±0.65), (3.06±0.33) mmol/L] and ε3 [(1.34±0.21), (1.00±0.28), (0.94±0.32) g/L] allele carriers in VaD group (P ＜ 0.05).CONCLUSION: Apolipoprotein E gene polymorphism is closely associated with the onset of VaD, for which ε4 allele might be a risk factor with similar impact in CI.

This paper introduces a microtiter-plate scanning instrument for medical laboratory equipment,which is controlled by the embedded PC when moving along the X direction and Y direction on the microtiter-plate plane. The designs of the driving circuit and orienting circuit are included in this paper. This instrument has been used in PKU fluorescence laboratory equipment for the newborn baby.

Objective To explore the possibility of using HBV as a gene delivery vector, and to test the anti-HBV effects by intracellular expression of antisense RNA. Methods Two parts of HBV genome were reversedly recombined back into overlength HBV genome, which can produce HBV particle, to express antisense RNA complementary to S or S promoter region respectively. HepG 2.2.15 cell lines were transfected with these constructs and the empty vector pMEP4, then positive clones were selected and mixed in respective groups with hygromycin in the culture medium. HBsAg and HBeAg, which exist in the culture medium, were tested by ELISA method and intracellular HBc related HBV DNA was examined by dot blot hybridization. The existence of recombinant HBV virion in the culture medium was examined by PCR. Results The mean inhibitory rates of HBsAg were (2.74?3.83)%、(66.54?4.45)%(P

Objective To analyze the clinical characteristics,diagnosis,differential diagnosis and treatment of gastrointestinal schwannoma.Methods Clinical data of 3 patients with gastrointestinal schwannoma were collected and retrospectively analyzed.Results Gastrointestinal bleeding or melena,anemia and epigastric pain were the most common presenting symptoms.The symptoms,physical signs and auxitiary examinations (such as X-ray,ultrasonography and gastrointestinal endoscopy) of gastrointestinal schwannoma had no value in differentiation.In all the 3 patients definite diagnosis was achieved only by postoperative pathology.Conclusion Gastrointestinal schwannoma are derived from the Schwann cells of nerves in gastrointestinal wall and are usually benign.Benign schwannomas can only be distinguished from the malignant ones on the basis of histological and immunohistochemical criteria.Surgical resection is the most effective treatment.