Objective To evaluate the clinical value of combined detection serum anti-HCG and estradiol and progesterone in infertile women.Methods Serum estradiol(E2) and progesterone(P) in infertile women were detected by ECLIA , Anti-HCG were detected by ELISA.Results the levels of estradiol(E2) and progesterone(P) in anti-HCG positive group were significantly lower than those in anti-HCG negative group.Conclusions The combined detection of anti-HCG and estradiol and progesterone were of important clinical value in diagnosis of infertile women.

OBJECTIVE To understand the distribution of pathogen in very-low-birth-weight or preterm infants septicemia in early-onset and late-onset and drug sensitivity.METHODS Bacterial isolates from inpatients of very-low-birth-weight or preterm infants septicemia over a period of 3 years were retrospectively analyzed,and classified as when septicemia was happened.RESULTS The positive rate of isolates was 43.2% in 970 samples of very-low-birth-weight or preterm infants septicemia.Most of the sepsis detected by blood culture was late-onset neonatal sepsis(58.6%).Pathogen of late-onset neonatal sepsis mostly was Gram-positive cocci,Staphylococcus were found to be the most common isolates(80.5%).In early-onset sepsis group,the isolates rates of Gram-negative and Gram-positive bacteria were mostly in proportion,there were 52.4% and 47.6%,respeitively.The resistance rate of late-onset sepsis group was higher than that of early-onset one.CONCLUSIONS The key of curing infants septicemia is that we should master the distribution of pathogen of very-low-birth-weight or preterm infants septicemia in early-onset and late-onset and drug sensitivity.Antimicrobial therapy should be initiated under the guidance of anti-microbial sensitivity test,in order to avoid abuse of antimicrobial.

OBJECTIVE To investigate the distribution and drug resistance of pathogenic bacteria which caused respiratory infection among suscepted patients and offer scientific basis for reasonable usage of antibiotics.METHODS Oropharyngeal swabs among 709 cases of respiratory infection neonates were investigated by the routine methods and drug resistance was analyzed by K-B method.RESULTS Totally 438 bacterial strains were isolated from 709 neonates.most of these bacteria were Gram-negative bacilli(70.3%),among which Haemophilus influenzae and Klebsiella pneumoniae were accounted for 39.8% and 10.3%,respectively;fungi and Gram-positive cocci were accounted for 23.5% and 6.2%.CONCLUSIONS Most strains present higher resistance rates to penicillin and ampicillin;but cefoxitin,amikacin,vancomycin,imipenem and the third generation cephalosporins are revealed with higher sensitivity rates for pathogenic bacteria in newborns.

Objective To study the effects of preoperative autologous blood donation on involution of uterus of puerpera under cesarean section after delivery. Methods Non-autologous blood donation was applied as control group. Height of uterus declining, pain of uterine contraction, amount of lochia, ending time of lochia and involution of uterus under ul-trasound B after delivery were compared between both groups. Results Heigh of uterus, score of uterine contraction pain, mean ending time of lochia, the sum of three dimensions of uterus in the third day and the seventh day and inci-dence of endometrial cavity fluid in the preoperative autologous blood donation group were lower than those in the non-autologous blood donation group. Mean good rate of involution of uterus in 42 days after delivery was higher than that in the non-autologous blood donation group. All the differences were statistically significant (P<0.05). Conclusion Pre-operative autologous blood donation is able to promote the involution of uterus after delivery, alleviate postpartum con-traction pain and significantly shorten lochia time, which is worthy of promotion.

Blocking the programmed death-ligand 1 (PD-L1) on tumor cells with monoclonal antibody therapy has emerged as powerful weapon in cancer immunotherapy. However, only a minority of patients presented immune responses in clinical trials. To develop an alternative treatment method based on immune checkpoint blockade, we designed a novel and efficient CRISPR-Cas9 genome editing system delivered by cationic copolymer aPBAE to downregulate PD-L1 expression on tumor cells specifically knocking out Cyclin-dependent kinase 5 () gene . The expression of PD-L1 on tumor cells was significantly attenuated by knocking out , leading to effective tumor growth inhibition in murine melanoma and lung metastasis suppression in triple-negative breast cancer. Importantly, we demonstrated that aPBAE/Cas9-Cdk5 treatment elicited strong T cell-mediated immune responses in tumor microenvironment that the population of CD8 T cells was significantly increased while regulatory T cells (Tregs) was decreased. It may be the first case to exhibit direct PD-L1 downregulation CRISPR-Cas9 genome editing technology for cancer therapy. It will provide promising strategy for preclinical antitumor treatment through the combination of nanotechnology and genome engineering.