Objective:To investigate the expression of mesothelin mRNA (MESO mRNA) and tumor metastasis suppressor gene KISS1 mRNA in epithelial ovarian cancer (EOC) and its correlation with prognosis.Methods:From January 2017 to January 2019, 52 patients with EOC in Tangshan Maternal & Child Health Care Hospital were selected as the observation group, and 52 healthy controls were selected as the control group. The levels of MESO mRNA and KISS1 mRNA between the two groups were compared; the correlation of MESO mRNA and KISS1 mRNA and EOC were analyzed; the correlation between MESO mRNA and KISS1 mRNA and clinicopathological characteristics was analyzed, and the expression of MESO mRNA and KISS1 mRNA in patients with different prognosis was compared after followed up for 1 year.Results:The levels of MESO mRNA and KISS1 mRNA in the observation group were higher than those in the control group (1.41 ± 0.40 vs. 1.41 ± 0.40, 0.73 ± 0.08 vs. 0.54 ± 0.07), and the differences were statistically significant ( P<0.05). The area under the curve(AUC) of combined diagnosis of EOC with MESO mRNA and KISS1 mRNA was 0.892 (95% CI 0.816 - 0.944), which was higher than the AUC of MESO mRNA and KISS1 mRNA single diagnosis ,which were 0.798 (95% CI 0.708 - 0.870), and 0.812 (95% CI 0.723 - 0.882). The differences of blood MESO mRNA level in different pathological types, operations-pathological stage, pathological grade, tumor diameter, lymph node metastasis were statistically significant ( P<0.05); the differences of blood KISS1 mRNA level in different operations-pathological stage, pathological grade, tumor diameter, lymph node metastasis were statistically significant ( P<0.05). The blood MESO mRNA level of the dead was higher than that of the survivors(1.52 ± 0.17 vs. 1.38 ± 0.15), the blood KISS1 mRNA level was lower than that of the survivors (0.69 ± 0.07 vs. 0.74 ± 0.06), and the differences were statistically significant ( P<0.05).The 1-year survival rate of patients with high expression of blood MESO mRNA was lower than that with low expression, and the 1-year survival rate of patients with high expression of KISS1 mRNA was higher than that with low expression ( P<0.05). Conclusions:The expression of MESO mRNA and KISS1 mRNA in the peripheral blood of patients with EOC is increased, but with the progression of the disease, MESO mRNA is increased and KISS1 mRNA is decreased. The abnormal expression of the two may participate in the development of EOC.

Objective To study the influence of Fuzheng Tixie Souluo Fang(Decoction of reinforcing healthy qi,eliminating pathogen and Searching collaterals,FTSF)on angiogenesis in rats with pulmonary fibrosis(PF),and investigate its latent therapeutic mechanism.Methods Health male SD rats were randomly divided into control group,model group and treatment group(each n=32).The PF model was established by intravenous injection of bleomycin via tracheal intubation in model group and treatment group,and control group was given intratracheal injection of isovolumetric normal saline.After modeling for 24 h,treatment group was given orally suspension of FTSF[0.67g/(kg· d)],and control group and model group were given isovolumetric normal saline.All rats were executed respectively at different time points(7 d,14 d,21 d and 28 d,8 each time)after modeling.The lung tissue was collected and given HE and Masson staining for detecting the expressions of vascular endothelial growth factor -A(VEGF-A) and its receptor-VEGFR-2 by using ELISA.The expression of CD34 antigen in lung tissue was detected by using immunohistochemistry(IHC)staining, and micro-vessel density was measured by using Wei-dner method.Results The pathological changes of lung tissue in treatment group were similar to those in model group,but severity of inflammation and fibrosis was milder than those in model group at the same time point.The expressions of VEGF-A and VEGF-R2 were lower in treatment group than those in model group at the same time point(P<0.01 or P<0.05).The staining of CD34 positive cells showed pale brown or brown,and micro-vessel density was lower in treatment group than that in model group(P<0.01 or P<0.05).Conclusion FTSF can relieve inflammatory exudation and collagen deposition of lung tissue induced by bleomycin in PF rats.The mechanism may be related to the decreases of VEGF-A and VEGF-R2 expressions and inhibition of abnormal angiogenic activity in lung tissue.

Objective:To reveal the mechanism of Mahuang Lianqiao Chixiaodou decoction and its disassembled formula for improving the skin barrier function in a mouse model of atopic dermatitis (AD).Methods:Sixty specific-pathogen free male BALB/c mice were randomly divided into the control group,model group,whole formula group (WF),exterior-releasing formula group (ERF),interior-clearing for-mula group (ICF),and positive control group (PC).A mouse model of AD was established using the semi-antigen 2,4-dinitrofluorobenzene induction method.The lesion scores,transepidermal water loss and pH,and skin histopathology of mice in each group were observed.The expressions of filaggrin,loricrin,and involucrin were detected by the streptavidin peroxidase immunohistochemical method and western blotting,and their mRNA expressions were detected by quantitative polymerase chain reaction.Results:Mice in the WF,ERF,ICF,and PC groups showed reduced skin lesion performance,improved histopathology,decreased skin lesion score,transepidermal water loss and pH,and upregulated ex-pressions of proteins including filaggrin,loricrin,and involucrin,and their mRNAs.The most obvious regulatory effect was observed in the WF group,followed by the ICF,ERF,and PC groups,accordingly.Conclusions:Mahuang Lianqiao Chixiaodou decoction and its disassembled formula can improve the skin barrier function in a mouse model of AD by upregulating filaggrin,loricrin,and involucrin,and their mRNA expressions,and the most optimal effect was noted in the WF group,followed by the ICF and ERF groups,which suggests that the effect of clearing heat and resolving dampness in improving the skin barrier function of AD is more obvious and is one of the key treatments for AD.

Objective To explore the predictive value of systolic pulmonary artery pressure (SPAP) on autonomic nerve excitation in patients with valvular disease, so as to provide reference for the formulation of clinical intervention plans. Methods The clinical data of patients with valvular disease who received surgical treatment in the General Hospital of Northern Theater Command from August 28, 2020 to February 3, 2021 were prospectively collected. According to the standard deviation of normal-to-normal R-R intervals (SDNN) of the heart rate variability (HRV) of the long-range dynamic electrocardiogram (ECG) 7 days before the operation, the patients were divided into three groups: a sympathetic dominant (SE) group (SDNN≤50 ms), a balance group (50 ms

Objective    To analyze factors affecting the recovery of postoperative left ventricular function in patients with valvular disease combined with heart failure with reduced ejection fraction [HFrEF, left ventricular ejection fraction (LVEF)<40%]. Methods    The clinical data of 98 patients with valvular disease combined with HFrEF who underwent surgeries in our hospital from January 2011 to June 2018 were retrospectively analyzed, including 75 males and 23 females aged 9-78 (55.3±11.9) years. Results    A total of 15 patients were dead after the operation, including 4 deaths within 3 months and 11 mid-long-term deaths after the operation. Ninety-one patients were followed up for more than 6 months (10 months to 8.6 years). The postoperative cardiac function (NYHA) of 91 patients was classⅠ-Ⅱ, the LVEF of 18 (19.8%) patients increased more than 10%, that of 47 (51.6%) patients maintained at the preoperative level, and that of 26 (28.6%) patients decreased. Postoperative LVEF was more prone to recover in HFrEF patients with sinus rhythm before operation (P=0.038), valvular disease mainly in aortic valve (P=0.026), obvious reduction of left ventricular end diastolic diameter in early postoperative period (P=0.017), and higher systolic pulmonary artery pressure (SPAP) before operation (P=0.018). The risk factors for postoperative LVEF deterioration included large left atrium before operation (P=0.014), smaller left ventricle end systolic diameter before operation (P=0.003), and fast heart rate after operation (P=0.019). Conclusion    Mitral valve prolapse patients with obviously increased left ventricular diameter should receive operation as soon as possible. HFrEF patients with aortic valve disease should receive operation positively. The operation efficacy is satisfactory in the HFrEF patients with high SPAP.