Objective To investigate the clinical application of the fine needle aspiration on spleen by ultrasonic guidance.Methods The fine needle aspiration by ultrasonic guidance was performed on 36 patients with spleen diseases.Among them,29 patients underwent diagnostic puncture,and the other 7 patients underwent remedial puncture.The definite diagnosis rate of the diagnostic puncture group was calculated by organism pathology and cytology result,as well as final diagnosis after the operation,while the curative effect of the remedial puncture group was appraised by re-examination after the operation.Results The definite diagnosis rate of the diagnostic puncture was higher,and the definite diagnosis rate of histology biopsy group(85.7%)was higher than that of cytology aspiration group(62.5%).All the patients who took remedial puncture were cured.Conclusion The fine needle aspiration on spleen by ultrasonic guidance has very important practical value in identifying the diagnosis of spleen disorders and treating fluidity processes of spleen.

Objective To study the induction of nitrous oxide(N2 O)and to compare safety and effec-tiveness of conscious sedation by nitrous oxide inhalation and intravenous sedation with propofol for upper gas-trointestinal(UGI)endoscopy.Methods A total of 400 patients undergoing UGI endoscopy in our hospital from April 2013 to October 2013 were randomly assigned to two groups,N2 O inhalation group(n=200)and in-travenous propofol group(n=200).The systolic pressures,diastolic pressures,heart rates and oxygen satura-tion,onset time,procedure duration,degree of sedation,recovery time,length of hospital stay,complica-tions,satisfaction ratings of doctors and patients,and the number of patients willing to accept the examination again were analyzed.Results The mean time of ideal anesthesia state for N2 O was (3. 16 ±0. 65 )min and there was no difference between the male and female(3. 16 ±0. 71)min vs.(3. 16 ±0. 58)min,t=0. 006,P>0. 05).The mean concentration was (43. 68 ±5. 05 )%,which was higher in male than that in female [(45. 3 ±4. 99)% vs.(41. 46 ±4. 30)%,t=3. 042,P<0. 05].Compared with before,the systolic pres-
sures,diastolic pressures,heart rates and oxygen saturation of patients in the propofol group significantly re-duced during the procedure(P<0. 05),while the same measurements excluding oxygen saturation for those in the N2 O group significantly increased(P<0. 05 ).Compared with the propofol group,patients inhaling N2 O had significantly shorter recovery and hospital stay time,but a longer onset time and procedure duration(P<0. 05 ).Complications that occurred in some patients of propofol group included hyoxemia,hypotension,brady-cardia,while the major complication in the N2 O group was nausea.The satisfaction ratings of doctors or pa-tients and the number of patients willing to accept the examination again in N2 O group were smaller than those in propofol group(86 ±3. 7 vs.96 ±2. 6,87 ±2. 8 vs.98 ±1. 2,87%vs.99%,P<0. 05).Conclusion Both conscious sedation by N2 O inhalation and intravenous sedation with propofol are effective for diagnostic UGI en-doscopy,but the safety of the former is superior to the latter.The key to complete the conscious sedation by ni-trous oxide inhalation is to accurately identify the ideal anesthesia state of N2 O.It is significant to pay attention to the nine factors concerning safe and effective sedation during operation.

Objective To detect the mitochondrial microsatellite instability (mtMSI) and Helicobacter pylori (Hp) infection status in esophageal squamous cell carcinoma (ESCC),and to analyze their clinical significance in the diagnosis of ESCC.Methods The mtMSI and Hp infection status were examined by immunohistochemical SP method in 93 cases of ESCC and their normal control tissues.The relationship between Hp and mtMSI and their correlations with the clinicopathological features of ESCC were analyzed.Results The Hp positive rates of ESCC group and control group were 61.3％ (57/93) and 20.4％ (19/93) respectively,and there was statistically significant difference between the two groups (x2 =32.127,P ＜0.001).The mtMSI positive rates of ESCC group and control group were 34.4％ (32/93) and 0 (0/93) respectively,and there was statistically significant difference between the two groups (x2 =38.649,P ＜0.001).The Hp infection was correlated with tumor infiltrating degree (x2 =22.213,P ＜ 0.001) and lymph node metastasis (x2 =8.318,P =0.004),but was not correlated with gender (x2 =0.330,P =0.565),major axis of tumor (x2 =0.692,P =0.406),gross type (x2 =1.006,P =0.316),differentiated degree (x2 =0.665,P =0.415).The mtMSI was not correlated with gender (x2 =0.163,P =0.686),major axis of tumor (x2 =0.384,P =0.530),gross type (x2 =0.422,P =0.516),differentiated degree (x2 =0.213,P =0.645),infiltrating degree (x2 =0.001,P =0.979) or lymph node metastasis (x2 =0.039,P =0.843).The Hp infection was positively associated with mtMSI in ESCC (r =0.864,P =0.006).Conclusion The positive rates of Hp and mtMSI in ESCC are higher than those in normal tissues,and there is a close relationship between Hp and mtMSI.Hp is also associated with the progression of ESCC.