1.The Growth-Suppressive Effect of Adenovirus-mediated Wild-type p53 Transfection on Gastric Cancer Cell Line
Fenghua PEI ; Shanling GAO ; Lixia ZHAO
Journal of Chinese Physician 2001;0(10):-
Objective To study the inhibitory effects of wild-type p53 on gastric cancer cell lines BGC-7901 and to explore the importance of wild-type p53 in gene therapy for gastric cancer. Methods Adenoviral vector with exogenous wild-type p53 gene was transfected into the gastric cancer cell line BGC-7901 which harbours a mutation in codon 204 of the p53 gene.The transfer efficiency and expression effect of p53 were examined by immunohistochemistry and in situ hybridization. The growth of BGC-7901 cells in vitro was determined by cell counting and MTT assay. Flow cytometric analysis was used for observing cell cycle.Results Transfer efficiency reached 100% in BGC-7901 cells when adenovirus infection ability was over 100MOI. 3 days after transfection, the introduction of exogenous wild-type p53 into the tumor cells resulted in decreased expression of the protein of mutant p53 and increased level of mRNA of wild-type p53 gene. The growth of the BGC-7901 cells was significantly slower after transfection with wild-type p53 gene than that before transfection. The percentage of G 1/G 0 phase of the cells with exogenous wild-type p53 gene was much higher than that of the cells without exogenous wild-type p53 gene.It was 56 47% and 79 40% respectively(P
2.Application of G-CSF in the transplantation of stem cell
Shizhu JIN ; Mingzi HAN ; Fenghua PEI
Basic & Clinical Medicine 2006;0(03):-
Granulocyte colony-stimulating factor(G-CSF) is a specific hematopoietic regulating growth factor of granulocyte lineage.It can be used to treat different kinds of granulocytopenia.Recently a variety of basic and clinical researches reported that G-CSF can mobilize bone marrow stem cells and haemopoietic stem cells in the peripheral blood,which suggested a potential aproach of releasing、enriching、mobolizing、promoting migration and inducing cell differentiation in the stem cell transplantation.The recent application of G-CSF in stem cell transplantation is reviewed in this view.
3.Ratio of visceral fat area to body fat mass (VBR) is a superior predictor of coronary heart disease.
Binbin ZHANG ; Jiangshan HE ; Pei GUO ; Jianxiong WANG ; Chunjun LI ; Li ZHANG ; Congfang GUO ; Yirui GUO ; Fenghua GUO ; Mianzhi ZHANG ; Minying ZHANG
Chinese Medical Journal 2023;136(19):2380-2382