OBJECTIVE: To compare 3 different calculation methods in the determination of aspirin in the bicomponent (aspirin and salicylic acid) system. METHODS: Different concentrations of mixed standard solution of aspirin and salicylic acid were prepared and scanned under a wavelength ranged from 230nm to 350nm. The absorbabilities of the 2 constituents were recorded at 265nm, 295nm and 320.5nm, and the data were treated with Iso-absorption diplo-wavelength method, proximal multiplying factor coefficient method and SA concentration fractional approximate calculation method. The recoveries of aspirin were calculated. RESULTS:The recoveries of aspirin determined by 3 different methods were (99.11?1.68)%, (98.82?1.17) % and (96.23?4.10)%, respectively. CONCLUSION: The proximal multiplying factor coefficient method is stable with little fluctuation in determination results, which proved to be simple while effective for the determination of sample at a great quantity.

During the development of pancreatic cancer,the local and whole immune state of the patients is constantly changing,and through which the cancer cells escape from the immune attack.The main immunologic mechanism includes the induction of tumor immune suppression cells and producing the related cytokines.The changes of the whole immune status include the increase of immunosuppressive cells,the change of the immune function of T cell subsets and the inhibition of natural killers and lymphokine activated killer cells.In the local tumor,the infiltrating lymphocytes loss the function,and the ratio of Thl/Th2 cytokines is imbalance.

Objective To investigate the effect of small interfering RNA (siRNA)-mediated Survivin knock-down on proliferation of human pancreatic cancer cell line Patu8988 and their sensitivity to Gemcitabi.Methods The siRNA against Survivin was constructed and transfected into Patu8988cells with LipofectamineTM 2000.The expression of Survivin was detected by RT-PCR and Western blot.Flow cytometry was used to detect the cell cycle.Sensitivity to Gemcitabi after transfection were examined by MTT and clonogenic assay.Results In Patu8988 cells,the protein and mRNA levels of Survivin were decreased significantly after transfection,gene expression:control group:0.78 ± 0.03,blank control group:0.82±0.06,experimental group:0.52 ± 0.05 ; protein expression were as follows:control group:0.77 ± 0.21,blank control group:0.77 ± 0.26,experimental group:0.57 ± 0.03,and the difference was statistically significant ( P ＜ 0.05 ).And reduction of proliferation was related to an increase in the fraction of G0/G1 phase.The sensitivity of Patu8988 cells to Gemcitabi was increased significantly after transfection.Conclusions The Survivin special siRNA silenced Survivin,decreased Patu8988 cells proliferation and enhanced their sensitivity to Gemcitabi.

Objective To determine the effect of neoadjuvant chemotherapy (NAC) for cervix cancer on cell proliferation and apoptosis. Methods A total of 24 patients with squamous carcinoma of the cervix (SCC) were treated with one cycle of cisplatin combined with hydroxycamptothecine, bleomycin regimen. The expression of proliferating cell nuclear antigen (PCNA) and apoptosis index (AI) were detected before and after NAC with flow cytometry and TUNEL. Results Clinical response was 58.3% (14/24). Before and after NAC, the expression of PCNA were (0.386?0.078) and (0.125?0.040) respectively and AI were (0.052?0.027) and (0.248?0.078). Significant difference of PCNA and AI could be observed before and after NAC (t=22.859, t=16.06, P

Objective:To investigate the clinical value of changes of platelet and anticoagulation function in patients with acute pancreatitis. Methods:β-TG, PF4 ,TXB2 ,GMP-140,antithrombin-Ⅲ,and protein C were measured in all patients. Results:There was no significant difference in all parameters between acute edema pancreatitis group and normal group(P＞0.05).Compared with the control group, parameters of platelet significantly increased in acute necrosis pancreatitis group(P＜0.01),and parameters of anticoagulation significantly decreased(P＜0.01). Conclusion: The platelet system was activated and the level of anticoagulation system decreased in acute necrosis pancreatitis. Parameters are important in understanding and preventing this disease.

Objective To study the distribution and significance of free organophosphorus poisons (FOP)with different degrees of hudrosolubility in the blood and adipose tissues of poisoned rabbits.Methods Seventy two male livid blue rabbits with 2-2.5 kg body weight were divided into 3 groups as per the rabbits intoxicated with different kinds of organophosphorous given subcutaneously,namely monocrotophos(11.12 mg/kg)group,trichloffon(556.0 mg/kg)group and methyl parathion(37.05 mg/kg)group(n =24 in each).Each group was further divided into 4 subgroups(n =6,in each).Blood samples and adipose tissues were collected 1 h,6 h,24 h and 96 h after administration of organophosphorous.Blood cells and plasma were separated.Well-formed adipose tissue homogenates were made.Acetylcholinesterase(AChE)activities were assayed with dithiobisnitrobenzoic acid(DTNB)enzyme kinetic method.The levels of FOPs in blood plasma,blood cells and adipose tissues were determined with enzyme inhibition method.Results There were signiflc.ant differences in FOP concentrations of plasma,blood cells and adipose tissues among moncrotophos group,trichlorfon group and methyl pararthion group at the intervals of 1,6 and 24 hours after organophosphorous administered(all P ＜ 0.05).There were no significant differences in FOP concentrations of moncrotophos among blood plasma,blood cells and adipose tissues at intervals of 1,6,24 and 96 hours.FOP concentrations of trichlorfon in adipose tissues were significantly higher than those in blood cells and plasma at intervals of 1,6,24 hours after organophosphorous administered(P ＜ 0.05).There were significant differences in FOP concentrations of pararthion in blood plasma compared with blood cells and adipose tissues at intervals of 1 and 6 hours after organophosphorous administered(P ＜ 0.05)and no difference was found over 24 hours after administration of organophosphorous(P ＞ 0.05).The significant differences in the rates of FOP clearance from blood and adipose tissues were also found between different organophosphates(P ＜ 0.05,moncrotophos ＞ trichlorfon ＞methyl pararthion).Conclusions With the decrease in hydrosolubility of organophosphates,the increase in adipose tissue-specific retention was found and the time required for clearance from blood and adipose tissue was prolonged.Adipose tissue was the major storage site for organophosphates with low hydrosolubility.

Objective　To evaluate the effects of vitrectomy associated with extraction of intraocular foreign bodies in the posterior segment.Methods　Retrospective analysis was done on clinical records of 11 patients (11 eyes) having undergone vitrectomy company with extraction of intraocular foreign bodies in the posterior segment.Results　The intraocular foreign bodies in the posterior segment were extracted successfully in all cases, the retinal detachment and endophthalmitis were cured. Postoperation visual acuity was improved in 8 eyes and did not change in 3 eyes. None suffered from postoperative secondary intraocular hemorrahge, decompensation of corneal endothelium and other serious complications.Conclusion　Vitrectomy combined with extraction of intraocular foreign bodies in the posterior segment is less damaging, accurate and safe, and enhances the cure rate. It is an effective method for treating intraocular foreign bodies.

BACKGROUND: Nowadays stem cells have been isolated successfully from the epithelial tissue of human and rodents such as skin, hair follicle, cornea, oral mucous, intestinal mucosa dental epithelial cells, and salivary glands by the Chinese or foreign researchers. But according to the vaginal mucosa stem cells (VMSCs), it is not known in the field of isolation and application.OBJECTIVE: To find out the suitable method of isolation, identification, and culture of human VMSCs.DESIGN: Observational basic study. SETTING: Department of Gynaecology and Obstetrics, the Third Affiliated Hospital of Guangzhou Medical College. MATERIALS: This study was performed in the Zhongshan Ophthalmology Center of Zhongshan Medical College from October 2004 to August 2005. Two-week gestation mice were provided by the Experimental Animal Center of Guangzhou University of Chinese Medicine. The sterile human vaginal mucosa was collected from five patients (25-38 years old) after the plastic operation of vagina from the 3rd Affiliated Hospital of Guangzhou Medical College. METHODS: Vaginal mucosa cells could be harvested from human vagina mucosa treated with trypsin-collagenase digesting. The VMSCs were selected by adhesion to type Ⅳ collagen for 20 minutes. The cell cycles of the VMSCs and primary human vaginal mucosa cells were analyzed by flow cytometry. VMSCs were cultured in vitro with 4 different media, including feeder layer cells plus epidermal total culture media, feeder layer cells plus DMEM/F12 (3:1), epidermal total culture media and DMEM/F12 (3:1). At the 12th day, their colony forming efficiency (CFE), CK19 and CK10 positive rates were compared. Then VMSCs were continuously cultured and passaged till they were apoptosis.MAIN OUTCOME MEASURES: ① Positive rate of CK19 and CK10 and G0/G1 proportion; ② Positive rate of CK19 and CK10, the colony forming efficiency (CFE) and the times be passaged when primary VMSCs were cultured in different media after 12 days. RESULTS: The cells at the G0-G1 phase cell cycle, CK19 and CK10 positive rates between the VMSCs and primary human vaginal mucosa cells were statistical different (P < 0.05). When cultured at 12th day, the VMSCs cultured with feeder layer and epidermal total culture media had the highest CFE and CK19 positive rate and lowest CK10 positive rate. They could be passaged over 15 times in vitro. CONCLUSION: It is an effective way that select VMSCs by adhesion to type Ⅳ collagen for 20 minutes. In this research, culturing VMSCs in vitro with feeder layer and epidermal total culture media was the best way to keep VMSC characteristics.

Objective To study the clinical curative effect of adefovir and lamivudine in the treatment of chronic hepatitis B .Methods 68 patients with chronic hepatitis B were randomly divided into the treatment group and control group,34 cases in each group.The treatment group was treated with adefovir and lamivudine for 48 weeks,the control group was treated by lamivudine for 48 weeks.12,24,36,48 weeks after treatment ,the changes of liver func-tion,ALT index in HBV DNA,HBV DNA and the changes of serum HBeAg negative rate ,HBeAg/HBeAb seroconver-sion rate and adverse reaction were observed and compared .Results With the extension of treatment time ,ALT,HBV DNA in the two groups were gradually decreased , there were significant differences before and after treatment ( t=4.63,4.65,P<0.05).24,36,48 weeks after treatment,ALT,HBV DNA in the treatment group were significantly lower than those in the control group[ALT (89.9 ±16.7) U/L,(60.1 ±13.8) U/L,(44.6 ±8.9) U/L vs (90.7 ± 17.6) U/L,(72.0 ±14.1) U/L,(61.3 ±24.5) U/L;HBV DNA (4.92 ±1.44) U/L,(3.35 ±1.37) U/L,(2.53 ± 1.31) U/L vs (5.02 ±1.41) U/L,(3.69 ±1.40) U/L,(3.02 ±1.35) U/L],there were significant differences between two groups(F=4.32,4.56.4.76,all P<0.05).After treatment,the HBV DNA and HBeAg negative con-version rate,HBeAg/HBeAb seroconversion rate in two groups were increased significantly ,the differences were statis-tically significant(χ2 =4.63,4.36,4.21,4.27,4,35,4.23,all P<0.05).At the 24th week of treatment,the serolog-ical markers of HBV had no significant difference , but after 24 weeks, serum markers in the treatment group (67.65%,32.35%,26.47%,85.29%,38.23%,35.29%) were obviously higher than those in the control group , the differences between the two groups were significant (χ2 =4.43,4.73,4.23,4.32,4.34,4.46,4.76,4.34,4.22, 4.29,4.34,4.45,all P<0.05).Conclusion Adefovir and lamivudine combination therapy in the treatment of chro-nic hepatitis B has obvious clinical effect ,has no serious adverse reactions ,which is suitable for clinical application .

Objective To study the dynamic changes of posttraumatic focal brain edema. Methods 120 patients who underwent multiple CT scans after head injury with hematoma or brain contusion in our hospital from 1998 to 2002 were enrolled in this study. Their CT image parameters and clinical data were recorded, and statistically analyzed. Results 96.7%(116/120) patients had perihematoma or pericontusion edema. Edema appeared 7 hours after head injury and reached its climax at the ninth day. Edema shape trended taper during its development, and cortex escaped from edema. Conclusion Focal brain edema was very common in the patient with head injury. Taper shape's formation and escape of cortex may mainly attribute to the difference in construction topology and blood supply between cerebral white and gray matter.