Objective To investigate the scavenging effect of Baoyuan anticancer oral solution on superoxide anion (O-2 ) and 1,1-diphenyl-2-picrylhydrazyl hydrate(DPPH) free radicals. Methods O-2 and DPPH were examined with the ESR (electron spin resonance) spin trapping method. Results The scavenging effect of Baoyuan anticancer oral solution diluted to 50 fold, on O-2 and DPPH free radicals was 81. 32% and 98. 47% , respectively. Conclusion Baoyuan anticancer oral solu-tion has a dose-related effect on free radical elimination.

Accurate pre-operative evaluation of characteristics and various pathological subtypes of renal parenchymal tumors has important value for the choice of operation design as well as the assessment of prognosis.The advances of CT,MR and contrast-enhanced ultrasonography (CUES) in diagnosis of renal parenchymal tumors were reviewed in this article.

Objective To determine the effect of neoadjuvant chemotherapy (NAC) for cervix cancer on cell proliferation and apoptosis. Methods A total of 24 patients with squamous carcinoma of the cervix (SCC) were treated with one cycle of cisplatin combined with hydroxycamptothecine, bleomycin regimen. The expression of proliferating cell nuclear antigen (PCNA) and apoptosis index (AI) were detected before and after NAC with flow cytometry and TUNEL. Results Clinical response was 58.3% (14/24). Before and after NAC, the expression of PCNA were (0.386?0.078) and (0.125?0.040) respectively and AI were (0.052?0.027) and (0.248?0.078). Significant difference of PCNA and AI could be observed before and after NAC (t=22.859, t=16.06, P

Objective To explore the origin of fluoride in the coal-combustion endemic fluorosis areas in the middle part of Hunan Province and to provide basic data for making preventive measure in the regions. Methods In November, 2006, the prevalence rate of dental fluorosis of children aged 10-12 years were examined, the villages were divided into three groups based on the prevalence rate of dental fluorosis. The fluoride content in the drinking water, coal, briquettes, briquette ash, clay and indoor air was determined by fluoride ion selective electrode. The quantity of fluoride release was calculated. Results There was no significant difference in the concentration of fluoride in water among the three groups, and it was below 1.0 mg/L in fluorosis and non-fluorosis areas. The fluoride levels in the coal, briquette ash and indoor air showed no statistical difference among groups. The fluoride levels of briquettes, clay and the quantity of fluorine release showed significant difference (P

Objective To explore the adverse effect of endemic fluorosis caused by fluoride from coal burning on the intelligence of children. Methods Four primary schools were selected respectively from slight, moderate, severe endemic fluorosis areas and the control area in Xinhua county of Hu'nan Province, and twenty children of grade 5 were randomly chosen in each school. The seriousness of dental fluorosis was examined by Dean's method and intelligence level was evaluated by CRT-RC. The morning urine was collected and the fluoride content in urine was measured by fluoride ion selection electrode method. Results The dental fluorosis was positively related to the fluoride content in urine (P0.05), but the difference was significant in the abilities of comparative inference, serial relationship and abstract inference (P

Objective To evaluate color Doppler sonography in the diagnosis of local recurrence in patients undergoing Miles procedure for rectal carcinoma.[WT5”HZ] Methods Transperineal and transsacral color Doppler blood flow sonography was performed on 51 patients presenting presacral masses after Miles procedure for rectal carcinoma. Blood flow richness and the blood flow dynamics indexes e.g. peak systolic velocity (PSV), diastolic velocity (EDV),resistive index (RI), and pulsatility indexs (PI) were recorded and analyzed. Results Blood flow signals in the recurrent masses were mainly Ⅱ-Ⅲ amounting to 81 82% (27/33), and that of benign masses were mainly 0-Ⅰ,Ⅱ-Ⅲ amounting to 27 78% (5/18). Both were significantly different (? 2=14 55, P

Objective To explore the effect of human neural stem cells(hNSCs) transplantation to treat cerebral ischemic rats and the status of transplanted hNSCs in the ischemic brain tissue of these rats.Methods Human neural stem cells were separated from 10~13 weeks brains of human embryo and were cultured and induced to differentiate. The middle cerebral artery occlusion rat models were made and the human neural stem cells were transplanted through tail vein 1 day later. Neurological Severity Scores (NSS) tests were undertaken in two groups after transplantation. Immunohistochemistry was used to check the differentiation and migration of human neural stem cells in vitro and vivo.Results Neural stem cells from human embryonic brains had been successfully cultured. These cells formed typical neurospheres in suspension, and the majorities expressed nestin. Three weeks later, the neurological function of rats that received transplantation recovered much better than the rats without transplantation, as evidenced by NSS ( P

Objective To explore the effect of human umbilical cord blood cells (HUCBCs) transplantation to treat cerebral ischemia of rats and the status of transplanted HUCBCs in the ischemic brain tissue of these rats. Methods The mononuclearcells abstracted from 60～100 ml of cord blood of full-term babies were cultured in vitro and marked with 5-bromodeoxyuridine (Brdu)(5 ?mol/L) for 2 days. The middle cerebral artery occlusion rat models were made and the HUCBCs (3?106) were transplanted into the lateral ventricular 1 day later. Neurological severity scores (NSS) tests were undertaken at different time point after transplantation, and iimmunohistochemistry method was used to check the migration and differentiation of HUCBCs. Results The HUCBCs had the capacity of proliferation in vitro and were induced to differentiate into astrocytes, oligodendrocytes and neurons in vivo. 3 weeks later, the neurological function of rats that received transplantation recovered much better than the rats without transplantation, as evidenced by NSS (all P

Objective To investigate the proliferation of neural stem cells by subcutaneous injection of basic fibroblast growth factor(bFGF),epidermal growth factor (EGF)or combined bFGF and EGF respectively after transient focal cerebral ischemia in rats.Methods The right middle cerebral arteries(MCA) of rats were occluded for 2 h to establish transient focal cerebral ischemia model. bFGF, EGF,combined bFGF and EGF or vehicle were administered subcutaneously at 24, 48 and 72 h after MCA occlusion(MCAO).Thereafter,one administration every 3 d. Proliferating neural stem cells were labeled with bromodeoxyuridine (Brdu) immunohistochemically. Brdu-labeled cells were calculated to characterize neural stem cells in SVZ and the dentate gyrus of the hippocampus at 7 d,14 d,21 d after MCAO.Results Brdu-labeled cells were detected in the bilateral SVZ and the dentate gyrus of the hippocampus after MCAO in each group, the number of positive cells decreased progressively. Compared with the control group, the number of Brdu-labeled cells in treatment group increased significantly after treatment( P