The genes of P53,retinoblastoma (Rb),C-myc and human papilloma virus (HPV) were determined by PCR method in specimens of 33 patients with laryngeal carcinoma and 4 normal laryngeal tissues as a control group.There were 23 males and 10 females,ranging in age from 40 to 11 years old.Results showed that the mutation rate of P53 and Rb gene were 69.69%(23/33) and 48.48%(16/33),respectively;c\|myc gene amplification rate and HPV insertion rate were 58.62% (17/29) and 72.72%(24/33),respectively.There were significant difference(P＜0.05)in comparison between positive and negative expression above these genes.The expression rate were 50.00%(12/24),45.83%(11/24)and 66.67%(16/24)of C-myc,Rb and P53 genes respectively in 24 cases who were HPV positive of laryngeal carcinoma.The positive rates were 73.68%(14/19),63.16%(12/19),57.89%(11/19)and 84.21%(16/19) of HPV,C-myc,Rb and P53,respectively in 19 cases who have heavy smoking (20/day,20 years smoking history).Above results suggested that there were closed relationship in the C-myc gene activation and P53,Rb genes inactivation with smoking and HPV infection.It was believed that carcinogen factors on laryngeal carcinoma was the result of factors,genes and stages interaction.

Objective To explore a simple and convenient surgical method for maxillary sinus cyst by endoscopic surgery. Methods Rudolf 4.5mm cannula paracentetic needle was used.The cyst was cut throuqh inferior nasal meatus and sampled. Results 30 cases were followed up for half a year to 3 years.No relapse was found either through X-ray or through CT scanning. Conclusions Exicision of maxillary sinus cyst by endoscopic surgery through inferior canal is one of minimally invasive operations,and the effect is positive.

OBJECTIVE To study the expression of matrix metalloproteinase 2 (MMP-2) and its correlation with microvascular density(MVD) and tumor metastasis in nasopharyngeal carcinoma. METHODS The expression of MMP-2 and MVD were detected in the specimens of 50 cases with nasopharyngeal carcinoma and 15 cases with nasopharyngeal inflammation by immunohistochemical technique. RESULTS The MVD in nasopharyngeal carcinoma(21.92?7.80) was significantly higher than that in inflammation nasopharyngeal tissues (9.23? 1.84, P

OBJECTIVE: To study the expression of tissue inhibitor of matrix metalloproteinase 2 (TIMP2)in the nasopharyngeal carcinoma and to evaluate the relationships between the expression of TIMP2 and biological behavior of NPC. METHOD: The expression of TIMP2 was observed by immunohistochemical technique in 50 cases with nasopharyngeal carcinoma and 15 cases of Inflammation nasopharyngeal tissues. RESULT: The positive rates of TIMP2 are significantly higher in NPC than in Inflammation tissues (P < 0.05). The expression of TIMP2 increase significantly with lymph node metastasis (P < 0.01), but had no relevant to the rise clinical invasion stage (P > 0.05). CONCLUSION The results indicate that TIMP2 may play a critical role in assisting carcinoma cell to degrade basement membrane to invade surrounding tissues and to form metastatic colonies in lymph node. Especially the unbalance in MMP2/TIMP2 has probably a prognostic value in nasopharyngeal carcinoma.
Adult ; Double-Blind Method ; Female ; Humans ; Male ; Matrix Metalloproteinase 2 ; metabolism ; Middle Aged ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Neoplasm Staging ; Tissue Inhibitor of Metalloproteinase-2 ; metabolism ; Young Adult