2.Chemical constituents of Dalbergia odorifera.
Hao WANG ; Wen-Li MEI ; Zhi-Kai GUO ; Zhan-Feng XIA ; Hui-Min ZHONG ; Hao-Fu DAI
China Journal of Chinese Materia Medica 2014;39(9):1625-1629
Fourteen compounds were isolated from Dalbergia odoriferae and purified by repeated column chromatography on silica and sephadex LH-20 gel and structurally identified by spectral analysis. These compounds were identified as 4, 9-dimethoxy-3-hydroxypterocarpan (1), medicarpin (2), 2', 4', 5-trihydroxy-7-methoxyisoflavone (3), 2', 3', 7-trihydroxy-4'-methoxyisoflavan (4), formononetin (5), 3, 8-dihydroxy-9-methoxypterocarpan (6), koparin (7), 3-hydroxy-9-methoxypterocarp-6a-ene (8), 2'-hydroxyformononetin (9), stevenin (10), 2', 7-dihydroxy-4', 5'-dimethoxyisoflavone (11), lyoniresinol (12), 2, 4-dihydroxy-5-methoxy-benzophenone (13) and neokhriol A (14). Compounds 1, 3, 4, 6, 8, 12 and 14 were isolated from this plant for the first time. Antibacterial activity assay showed that compound 4 had inhibitory effect on Ralstonia solanacearum.
Anisoles
;
chemistry
;
isolation & purification
;
pharmacology
;
Anti-Bacterial Agents
;
chemistry
;
isolation & purification
;
pharmacology
;
Benzophenones
;
chemistry
;
isolation & purification
;
pharmacology
;
Chromatography
;
methods
;
Dalbergia
;
chemistry
;
Dextrans
;
Gels
;
Isoflavones
;
chemistry
;
isolation & purification
;
pharmacology
;
Microbial Sensitivity Tests
;
Naphthalenes
;
chemistry
;
isolation & purification
;
pharmacology
;
Plant Extracts
;
chemistry
;
isolation & purification
;
pharmacology
;
Pterocarpans
;
chemistry
;
isolation & purification
;
pharmacology
;
Ralstonia solanacearum
;
drug effects
;
growth & development
;
Silica Gel
3.Prenatal diagnosis of prelingual deafness by determination of SLC26A4 gene mutation
Hao HU ; Lingqian WU ; Desheng LIANG ; Yong FENG ; Fang CAI ; Kun XIA ; Qian PAN ; Zhigao LONG ; Heping DAI ; Jiahui XIA
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
G mutation of SLC26A4, the parents and the second child were carriers of the same mutation, while the fetus had a wild-type form. Conclusion It is feasible to identify deafness related genes by screening for GJB2 and SLC26A4 mutation, thus providing correct prenatal diagnosis and avoiding deaf delivery of baby.
4.Application of dynamic pupillometer in the evaluation of pupillary light reflex in C57BL/6 mouse
Rui, LIU ; Lei, ZHANG ; Hao, ZHANG ; Wen-zhi, HU ; Feng, XIA ; Jia, GENG ; Zuo-ming, ZHANG
Chinese Journal of Experimental Ophthalmology 2013;(1):75-80
Background Pupillary light reflex has been widely used in the diagnosis and evaluation of visual system and nervous system diseases.However,in animal experiments,functional evaluation of the visual system and nervous system needs more advanced technology and are affected by many factors.Objective This study was to explore the use of the dynamic pupillometer in evaluating pupillary light reflex and to discuss the influence of brightness of stimulate on relevant curve parameters in C57BL/6 mouse.Methods Ten healthy SPF male C57BL/6 mice were collected in this experiment.White light of five luminance levels (2,8,32,128,256 cd/m2) was used to stimulate the mice following a 2-hour dark adaptation.The stimulation was given at the 60-second intervals,for a duration of 100 ms at every stimulation.An infrared camera and video capture card were used to capture digital images during the measuring process in a scotopic environment,at a speed of 60 frames per second.Measuring outcome was saved in the*.AVI format.A software that was developed by our group was used to determine pupil diameter and output pupillary light reflex curve offline.Pupil initial diameter (R1),constriction amplitude (CA),constriction velocity (CV),latency (T1),time for maximum velocity (T2),time for maximum constriction (T3),time for maximun con-striction to 10.1% R1 re-dilation (RT)and re-dilation velocity (RV)were assessed,and the correlations between luminosity and measuring parameters were analyzed using the Spearman rank correlation.The use of animals followed the Regulations for thd Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.Results R1 values showed no statistically significant difference among the 5 different luminosity groups(F=1.117,P=0.361).A positive linear correlation was found between stimulating luminosity and CA(r=0.508,P< 0.01),but negative correlations were seen between stimulating luminosity and CV or RV (r=-0.625,-0.609,P<0.01).T1 and T2 values in the 5 different luminosity groups were not statistically significant (F =0.202,P =0.936 ; F =1.584,P =0.195).The different levels of stimulating luminosity showed positive linear correlations with T3 and RT values (r =0.791,0.609,P< 0.01).Conclusions The dynamic pupillometer can quantitatively measure the pupillary light reflex of C57BL/6 mice.The pupillary light reflex dynamic curve parameters of mouse were affected by stimulus luminosity levels.These outcomes offer a basis for the application of the dynamic pupillometer system for measuring pupillary light reflex in animal models.
5.Preliminary study on validity and reliability of the chronic HBV-infections related stigma scale
Lifen FENG ; Junqiang XIE ; Xia ZOU ; Jingzhi HUANG ; Weilin GUO ; Qing GU ; Yuantao HAO
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(4):370-372
ObjectiveTo evaluate the reliahility and validity of the chronic HBV-infections related stigma scale.MethodsThe initial items and construct of the scale were developed according to theoretical analysis and interviews of experts and patients.A total of 151 patients with chronic HBV-infection were administered by convenient sampling method in this pilot study. The reliability and the validity of the scale were then evaluated.ResultsThe response rate of the scale was 94.5%.The Cronbach α coefficients of all dimeusions ranged from 0.75-0.87.The results of correlation analysis showed that there were higher correlation coefficients ( r ranged from 0.62-0.86) between items and their hypothesized subscales than those with other subscales ( r ranged from 0.14-0.55).The scale distinguished between patients with low subscale scores ( the subscale scores were ( 1.89 ±0.30 ),( 1.86 ± 0.29 ),( 1.96 ± 0.23 ),( 2.29 ± 0.45 ),( 1.59 ± 0.42 ) independently) and those with high subscale scores(the subscalc scores were (3.62 ±0.44),(3.99 ±0.41 ),(3.79 ±0.37),(4.13 ±0.34),(3.10 ±0.53 ) independently) (P < 0.01 ).Confirmatory factor analysis showed that the main indices of goodness of fit CFI was 0.94,NNFI 0.92,RMSEA 0.087.ConclusionThe chronic HBV-infections related stigma has good psychometric properties regarding to reliability and validity.
6.Application of detection of clonal immunoglobulin heavy chain gene rearrangement in paraffin-embedded tissues from B-cell non-Hodgkin lymphomas.
Xin-Xia LI ; Yun-Zhao CHEN ; Feng LI ; Wen-Hao HU ; Hong-An LI ; Jin-Fang JIANG
Chinese Journal of Pathology 2007;36(2):126-127
Adolescent
;
Adult
;
Aged
;
Aged, 80 and over
;
Child
;
Child, Preschool
;
DNA, Neoplasm
;
genetics
;
Female
;
Gene Rearrangement, B-Lymphocyte, Heavy Chain
;
Humans
;
Immunoglobulin Heavy Chains
;
genetics
;
Infant
;
Lymphoma, B-Cell
;
diagnosis
;
genetics
;
Lymphoma, Large B-Cell, Diffuse
;
diagnosis
;
genetics
;
Male
;
Middle Aged
;
Paraffin Embedding
;
Polymerase Chain Reaction
;
Young Adult
7.Mutation analysis of keratin 5 and keratin 14 genes in a family with epidermolysis bullosa simplex with mottled pigmentation.
Xia LIU ; Li XIA ; Jing-xia WANG ; Yan-jie HAO ; Jing YANG ; Feng-qin LIU ; Rui GUO
Chinese Journal of Medical Genetics 2011;28(6):612-615
OBJECTIVETo identify keratin 5 (K5) and keratin 14 (K14) gene mutations in a family affected with epidermolysis bullosa simplex with mottled pigmentation.
METHODSGenomic DNA was extracted from peripheral blood samples obtained from eleven patients from the family and controls. All the exons of K5 and K14 genes were amplified using polymerase chain reaction (PCR) and directly sequenced.
RESULTSBy DNA sequence analysis, a missense mutation in K5 gene (c.237C>T) was detected. The same mutation was not found in non-affected members from the family and normal controls.
CONCLUSIONMutation in K5 gene (c.237C>T) may be responsible for the development of disease in this family.
Base Sequence ; DNA Mutational Analysis ; Epidermolysis Bullosa Simplex ; genetics ; pathology ; Exons ; Female ; Humans ; Hyperpigmentation ; genetics ; pathology ; Keratin-14 ; genetics ; Keratin-5 ; genetics ; Male ; Mutation ; Pedigree ; Sequence Analysis, DNA
8.The investigation of HEV epidemiology, inferior clinical infection and viral-toxemia in blood donors in Quzhou area.
Xiao-hua HE ; Hao LV ; Jian-xun ZHEN ; Min-xia ZHU ; Feng WANG
Chinese Journal of Experimental and Clinical Virology 2008;22(1):45-47
OBJECTIVETo investigate the infection of HEV in Quzhou area of Zhejiang Province.
METHODSAll sera from blood donors in the central blood bank of Quzhou from April 2006 to April 2007 were used. Anti-HEV IgG and anti-HEV IgM were measured by EIA. RT-PCR was also performed to the samples with positive anti-HEV IgM. Genotype and sequence homology were analyzed after sequencing.
RESULTSThe positive ratio of anti-HEV IgG was 40.60%, in which the male infection ratio was higher than the female significantly (43.09% VS 36.09%; chi2=22.6; P < 0.01). The infection ratio was increased with age. The positive ratio of anti-HEV IgM was 0.43%. The positive ratio of anti-HEV IgG and the titers of antibody were higher in the inferior clinical infectors with positive anti-HEV IgM than the negative ones (P < 0.05). Two samples were positive in HEV PCR among 21 samples with positive anti-HEV IgM. The toxemia ratio was 0.4% of all the donors. And the genotype of the two samples with toxemia were both HEV-IV.
CONCLUSIONThe HEV infection was correlation with age and sex significantly and the infection occurred in the adults mainly in Quzhou area. HEV toxemia was not infrequency in the blood donors.
Adolescent ; Adult ; Age Factors ; Antibodies, Viral ; analysis ; immunology ; Blood Donors ; China ; epidemiology ; Female ; Genotype ; Hepatitis E ; complications ; epidemiology ; immunology ; virology ; Hepatitis E virus ; genetics ; immunology ; Humans ; Immunoglobulin G ; analysis ; immunology ; Immunoglobulin M ; analysis ; immunology ; Male ; Middle Aged ; Sequence Analysis, DNA ; Sequence Homology ; Sex Factors ; Toxemia ; complications ; epidemiology ; virology
9.The diagnostic values of multicolor melting curve analysis on drug resistance to 5 anti-tuberculosis drugs
CHANG Feng-xia ; NA Yuan-chun ; HAO Juan ; PENG Mao-cuo ; LUO Li-yuan ; MA De-zhao ; MA Ming
China Tropical Medicine 2023;23(4):409-
Abstract: Objective To explore and analyze the diagnostic value of multicolor melting curve analysis (MMCA) for the resistance of five anti-tuberculosis drugs, so as to clarify the clinical value of MMCA in detecting drug resistance of Mycobacterium tuberculosis. Methods From April 2021 to May 2022, 200 patients with positive Mycobacterium tuberculosis admitted to the Fourth People's Hospital of Qinghai Province were selected as research objects, and sputum specimens were taken from the patients. Traditional Mycobacterium tuberculosis drug sensitivity test (modified Löwenstein-Jensen medium method) and MMCA analysis were respectively given to detect the resistance of five anti-tuberculosis drugs, including isoniazid, ethambutol, streptomycin, rifampicin and isoniazid, respectively. Those samples with inconsistent results between the two diagnosis methods were subjected to gene sequencing verification, and the diagnosis efficiency of MMCA for the five anti-tuberculosis drugs was compared. Results Using Mycobacterium tuberculosis drug sensitivity as the gold standard for drug resistance diagnosis, the sensitivity of MMCA for detecting drug resistance of rifampicin, ethambutol, streptomycin, isoniazid and levofloxacin were 95.83% (46/48), 93.75% (15/16), 100.00% (15/15), 100.00% (20/20) and 70.00% (7/10), respectively, with statistical differences between groups (P<0.05). There were no statistically significant differences in the specificity, positive predictive value, negative predictive value and accuracy of MMCA for the five anti-tuberculosis drugs (P>0.05). For the 8 samples with inconsistent results between MMCA and modified Löwenstein-Jensen medium method, gene sequencing was performed and compared with the results of gene sequencing. After comparison with gene sequencing results, it was found that the coincidence rate of MMCA and gene sequencing results was 75.00% (6/8). Conclusions In the detection of drug-resistant mutations in TB patients, multi-color probe fusion curve analysis has high diagnostic efficacy for first-line anti-tuberculosis drugs, but is not sensitive to second-line anti-tuberculosis drug levofloxacin. Therefore, for the detection of first-line anti-tuberculosis drugs, MMCA has a good clinical application prospect.
10.The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure.
Zhao-xia QI ; Shu-xia YU ; Hong-sheng HAO ; Feng-cai LI ; Zhao-yang GUO ; Yu-chen FAN ; Kai WANG
Chinese Journal of Experimental and Clinical Virology 2011;25(2):99-101
OBJECTIVETo investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF).
METHODSPatients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of IL-10 promoter via MSP, to analyze the difference among the three groups.
RESULTSBoth the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group (P < 0.05); the ACLF group's level is higher than the CHB group, however without statistical significance (P > 0.05). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0.022, r = 0.033, respectively; P > 0.05); has positive relativity with TBIL and MELD ( r = 0.566, r = 0.443, respectively; P < 0.05); and negative relativity with PTA (r = -0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two.
CONCLUSIONThe serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.
Adolescent ; Adult ; Chronic Disease ; DNA Methylation ; Female ; Humans ; Interleukin-10 ; blood ; genetics ; metabolism ; Liver Failure, Acute ; blood ; genetics ; metabolism ; Male ; Methylation ; Middle Aged ; Promoter Regions, Genetic ; Young Adult