Antiviral Agents ; therapeutic use ; China ; Hepatitis B ; drug therapy ; epidemiology ; immunology ; prevention & control ; therapy ; Hepatitis B Vaccines ; immunology ; Humans

Sick sinus syndrome (SSS) is a disease of multiple arrhythmias and symptoms.It has great impact on patients in the quality of life for the symptoms of high incidence.The asynchrony of myocardial electromechanical movement caused by the SSS electrophysiological changes are focused.The ultrasound can not only observe the electrical physiological activity,but also measure the mechanical movement caused by electrophysiological delay.The research progresses of ultrasound in quantitative evaluation of myocardial function in SSS patients was reviewed in this article.

China ; epidemiology ; Hepatitis, Viral, Human ; epidemiology ; Humans

The antagonistic effects of phentolamin(PL) and tolasuline(TS) on norepinephrine(NE)-induced vasoconstriction were investigated in vitro on aortic rings of normotensive and spontaneously hypertensive rats in Kreb-Henseilet solution containing 40?IU/ml insulin.The results showed that in the presence of high level insulin,the antagonistic actions of ?-receptor blocking agents,PL and TS,were markedly decreased.The pharmacodynamic parameters indicated that the affinity index and action intensity of PL and TS were significantly reduced in solution containing high level insulin.The experimental results showed that high concentration insulin could decrease the antagonistic effects of ?-receptor blocking agents on NE-induced vasoconstriction.

Head and Neck Neoplasms ; diagnostic imaging ; surgery ; Humans ; Neck Dissection ; Positron-Emission Tomography

Objective:To investigate the effect of Yiqi Huoxue Jiedu on diabetic peripheral neuropathy.Methods: 60 patients were randomly divided into treatment group receiving Yiqi Huoxue Jiedu treatment,control group receiving vitamin B and routine treatment to improve neural microcirculation.The conduction velocity of all patients’sensory and motor nerves and the scores of the symptoms had been observed.Results: Symptoms and nerve conduction velocity of treatment group and control group had been improved respectively,and the treatment group was superior to control group in clinic symptoms,clinical obvious effective rate and total effective rate.Conclusion: Yiqi Huoxue Jiedu method can improve conduction velocity of sensory and motor nerves,symptoms of anesthesia and pain of limbs of patients with diabetes peripheral neuropathy,and have good effect on diabetes peripheral neuropathy.

Objective To study and estimate the effective dose of interventional employees in the common cerebralvascular, cardiovascular and liver interventional diagnosis and treatment.Methods The absorbed doses of tissue or organ of anthropomorphic phantom in these three procedures were estimated by the anthropomorphic phantom experiment.The effective doses were calculated by the tissue weight factor which was given by International Commission on Radiological Protection publication 103.Results The effective doses to high, medium and low group were 24.0, 9.7,6.8 μSv for cerebralvascular interventional diagnosis and treatment, and 36.3, 29.3, 17.8 μSv for cardiovascular interventional diagnosis and treatment, and 23.9, 11.3, 5.5 μ Sv for liver interventional diagnosis and treatment, respectively.Conclusions The effective doses of high, medium and low group of interventional employees in cardiovascular interventional procedure are higher than those of cerebralvascular and liver interventional procedures.

BUMwith high insulin level.

ObjectiveTo investigate the clinical value of serum complement C3 and C4 levels for predicting the severity of hepatic fibrosis in patients with chronic hepatitis B.MethodsHistopathological diagnosis was confirmed in 442 patients with chronic hepatitis B.Serum complement C3 and C4 levels were determined by Beckman-Coulter Immage 800 immunochemistry system.ROC curve was used to analyze the value of serum complement C3 and C4 levels in predicting the severity of hepatic fibrosis.ResultsThe areas under ROC curve of complement C3 and C4 for predicting significant fibrosis ( ≥ S2),severe fibrosis ( ≥ S3) and cirrhosis (S4) were all significantly larger than the area under diagonal reference line ( P =0.009,0.000,0.000 and P =0.005,0.000,0.000,respectively).According to ROC curves,the optimal cut-offs of serum complement G3 for predicting severe fibrosis and cirrhosis were ≤0.74 g/L and ≤0.64 g/L,and the corresponding sensitivity,specificity,positive predictive value,negative predictive value,accuracy were 0.585,0.681,0.617,0.650,0.636 and 0.509,0.775,0.423,0.830,0.710,respectively.The optimal cut-offs of serum complement C4 for predicting severe fibrosis and cirrhosis were ≤0.14 g/L and ≤0.12 g/L,and the corresponding sensitivity,specificity,positive predictive value,negative predictive value,accuracy were 0.565,0.634,0.576,0.623,0.602 and 0.463,0.781,0.407,0.818,0.704,respectively.ConclusionSerum complement C3 and C4 may be used for predicting severe fibrosis and cirrhosis in patients with chronic hepatitis B,but its stability and reliability need to be improved.

Objective:To prepare the egg yolk immunoglobulin Y ( IgY) against human Sucrase and study its stability,in vitro activity. Methods:Hy-line laying hens were immunized with human Sucrase protein,IgY was isolated and purified from egg yolks of im-munized hens using water dilution and salting out method. Indirect ELISA was used to evaluate the titer and stability of IgY. The purity and specificity of IgY were analysed by SDS-PAGE and Western blot respectively. The inhibitory effects of IgY on α-glucosidase was studied by PNPG method. Results:Indirect ELISA results showed IgY could be detected on the tenth day after the first immunization, and the peak titer of IgY was 1:12 800 after the 40th day of immunization. SDS-PAGE showed that the heavy chain and light chain of IgY were 65 kD and 25 kD respectively, and the IgY against human Sucrase could specifically recognize the protein of human Sucrase. The IgY maintained primary titer when it was kept between 29-69℃ for 15 min,and pH 4-7,37℃,4 h. The titer of IgY was maintained 50% after digestion by pepsin and trypsin respectively for 2 hours. IgY had a higher resistence to pepsin than trypsin after longer digestion time. IgY showed an inhibitory effect on α-glucosidase in concentration dependent manner. The half inhibitory concentration (IC50) was 0. 540 mg/ml. Conclusion:The IgY against human Sucrase has been successfully obtained,which established foundations for its study of Type 2 diabetes mellitus rat models in vivo.