Purpose:Our study is to find out the inhibitory action of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) on ovarian cancer cell line cultured in vitro. Methods:MTT was applied to assay the inhibiting action of various concentration of TRAIL on two ovarian cancer cell lines of 3AO and HO-8910.The apoptosis rates were measured by flow cytometry. Results:The growth of human ovarian cancer cells was effectively inhibited by TRAIL. A clear dose- and time-dependent correlation between TRAIL concentration and the degree of apoptosis induction was observed with up to 43.20% apoptotic cells after 24 h of incubation with 50 ng/ml TRAIL. The cells assumed typical cell apoptosis configuration. Conclusions:TRAIL can effectively inhibit the growth of ovarian cancer cells and induce apoptosis of the cells.

Objective The aim of this study is to elucidate the current status of hilar cholangiocarcinoma with regard to its epidemiology,clinical features,diagnosis,Bismuth-Corlette typing,pathologic categories,treatment,complication and prognosis in China.Methods Original articles published form January 1991 to August 2008 were searched in the CBM disc,VIP information and CNKI.Clinical appraisal and data extraction were conducted independently by 2 reviewers.The outcomes of 2280 procedures from 22 retrospective studies were analyzed.Results When radical resection was compared with palliative resection,the 1-yr survival rate (84.0％ vs.46.8％,OR=5.71,95％CI 3.99～8.17,P＜0.01) and 3-yr survival rate (31.5％ vs.13.5％,OR=4.43,95％CI 2.07～9.47,P＜0.01) were significantly higher.For the palliative resection group,the 1-yr survival rate of the drainage group (27.2％ vs.38.7％,OR=0.47,95％CI 0.31～0.72,P＜0.01) was significantly higher than the palliative resection group.The rate of tumor resection rose after 2002 (x2=35.9588,P＜0.01),but the rate of radical resection did not significantly rise ((2 =2.1052,P=0.1468).Conclusions Radical resection is still the optimal treatment for hilar cholangiocarcinoma.If radical resection is technically not possible,palliative drainage is a reasonable treatment.Palliative resection do not improve survival and it is not recommended.

Premature ejaculation (PE) is a most common sexual dysfunction, for which dapoxetine, a novel selective serotonin (5-HT) re-uptake inhibitor (SSRI), is the only licensed oral medicine at present. With the advantages of fast absorption, rapid action, on-demand medication, and short half-life time, dapoxetine has been proved by clinical trials to be effective in prolonging the intravaginal ejaculation latency time (IELT) and improving the overall condition of PE patients in various areas and populations. Compared with the traditional SSRIs, dapoxetine has a better safety and tolerability. The most frequently reported dapoxetine-related adverse events include nausea, diarrhea, headache and dizziness, but with very few severe or serious cases.
Benzylamines ; therapeutic use ; Biomedical Research ; Ejaculation ; drug effects ; Humans ; Male ; Naphthalenes ; therapeutic use ; Premature Ejaculation ; drug therapy ; Reaction Time ; drug effects ; Serotonin Uptake Inhibitors ; therapeutic use ; Treatment Outcome

Objective To investigate urinary tract injuries in laparoscopic-assisted vaginal hysterectomy(LAVH).Methods Clinical data of 5 cases of urinary tract injuries from 415 cases of LAVH from December 1995 to January 2006 in this hospital were retrospectively studied. Results Intraoperative bladder injury occurred in 2 cases and an immediate repair was given.Postoperative ureteral injury occurred in 3 cases: 2 cases of ureteral injury were found 4 days after operation because of abdominal pain,and 1 case was found on the 30th day after operation because of massive vaginal discharge.Conclusions Urinary tract injury is one of common complications in laparoscopic-assisted vaginal hysterectomy.High-risk factors include tumor size more than 5 cm,tumor protruding to the broad ligament,and tumor adjacent to the isthmus.Surgical repair is the major treatment.

A study on 48 middle-aged and elderly joggers through exercise echocardio-graphy and measurements during systolic time intervals (STI) showed that whencompared with a control group of 26 sedentary persons, their stroke volume was44.2% and cardiac output 25.1% greater at rest, 34.3% and 15.8% greater duringequivalent load exercise, and 35.1% and 33.1% grater during equivalent heart rateexercise. In addition, their left ventricular end-diastolic diameter (LVEDd) andleft auricule diameter were also longer and the closing velocity of the mitral valveduring the early diastolic stage was greater, although their ejection fraction, AD%and STI showed no significant difference. This proves that jogging can improvecardiac pumping function by enlarging LVEDd and increasing pre-load of heart.

Objective: To investigate microencapsulation technique, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after the single-dose HBsAg-PLGA microspheres subcutaneously injected (sc) to BALB/c mice. Methods: HBsAg-PLGA microspheres were prepared by double emulsion microencapsulation technique with orthogonal experiments. The pharmaceutical characteristics of size and surface morphology, antigen loading efficiency, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after single sc of PLGA microspheres in BALB/c mice were investigated. Results: The concentration of PVA was the significant factor affecting the particle size (P

Objective To register two breath-hold lung volumes image from one subject with deep expiration and deep inspiration.Methods Three pairs of thoracic high resolution CT serial from three subjects were collected under two breath-hold respiration stages.The lung parenchyma of every serial was segmented using the serial segmentation algorithm.Left and right lungs were stored separately.Expiration and inspiration volume images of single lung were registered.Firstly,affine transformation parameters were found based on the anatomic flag surfaces and expiration image volume was re-sampled with affine transformation.Secondly,"Demons" algorithm was employed to register two image volumes non-rigidly.Results Two lung surfaces and the inner structures have a nice registration.The average volume overlap of two images before registration is 0.7982.After global affine transformation,it improves to 0.8936.After "Demons",it is up to 0.9544.The average descending percentage of root mean square errors is 19.83%(after the global affine transformation) and 49.43%(after the "Demons" non-rigid registration).Conclusion The intra-subject registration between two lung image volumes with large deformations described here has an effective registration result.It offers a good base to analyze the lung respiration function.

Objective: To study the influence of ligating different sites of carotid arteries on face and nose blood flow in rabbits. Methods:Different levels of carotid arteries were ligated using laser Doppler flowmetry to observe the change of blood flow in face and nose regions before and after operation. Results: Blood flow of rabbit nose was more than that of the face region. Among the four operations of ligating different blood vessels to reduce blood flow of face and nose region, the most effective method was to ligate ipsilateral carotid artery and its branches on the same side. Conclusion: Ligation of some relating blood vessels, resulting in the establishment of collateral circulation, and the blood flow is quicker in the early stage. [

Cell viability of Pichia pastoris was detected by flow cytometry (FCM) with two reagents fluorescein diacetate (FDA) and propidium iodide (PI). Compared with FDA/PI double-stained dot plots and PI single-stained dot plots,the latter could divide dead and living cells into two separate zones,and get the correct proportion. Then PI single-stained method was used to detect the change of cell viability in Pichia patoris fermentation. At glycerol batch and fed-batch phase,little dead cells were detected. At methanol fed-batch phase,cell viability decreased when cell weight increased,and was only 73.8% at 88 h.

Objective:To investigate the causes of false-positive anti-HCV in non-syndromic cleft lip and palate with serum chemiluminescence test. Methods:We performed a hospital-based,case-control study of 4 050 patients with non-syndromic cleft lip and palate and 8 547 control patients without cleft lip and palate to evaluate associations between positive anti-HCV and non-syndromic cleft lip and palate. CLIA test were used for detect anti-HCV in control group. RIBA and FQ-PCR were used for confirming the result of the positive samples of CLIA test. CLIA test were used for detect anti-HCV in case group until CLIA test was negative. IgG,RF and ANA were used for case-control comparisons. Results:The detection rate of anti-HCV were higher in the case group than that of control group (P<0. 05). The false-positive rate was 1 percent based on testing of 4 050 patients. These samples were negative after opration 1-19 months. There were no change among IgG, RF and ANA in the false-positivepatients. Conclusion: False-positive anti-HCV in non-syndromic cleft lip was possible associated with abnormal plasma metabolic product. It can be excluded by track monitoring.