Objective To investigate the effects of the serum from chronic renal failure (CRF) rats on endoplasmic reticulum stress (ERS) and activation of activating transcription factor 4 (ATF4) of rat arterial smooth muscle cells (ASMCs) cultured in vitro and explore the possible mechanism. Methods The rat model of CRF was established by 5/6 nephrectomy. ASMCs were incubated in the media with 10% or 20% serum of CRF rats cultured in vitro. ATF4 nuclear translocation were analyzed by immunofluorescence. GRP78 (glucose regulated protein 78), p-PERK (pancreatic ER kinase) and ATF4 proteins in response to the CRF serum in ASMCs were determined by Western blot. The ATF4-DNA binding activity was analyzed by electrophoretic mobility shift assay (EMSA). Results The CRF serum could significantly promote the expression of GRP78 of ASMCs in dose-dependent manner. Under the stimulation of 20% CRF serum, nuclear translocation of ATF4 in ASMCs was found. Compared with control serum group, the expression of ATF4 and p-PERK of CRF serum group was increased significantly (P<0.01). The CRF serum could increase the ATF4-DNA binding activity. Conclusion The CRF rat serum can effectively induce ERS of ASMCs and activate the pathway of PERK-ATF4.

Animals ; DNA Damage ; DNA Repair ; DNA-Activated Protein Kinase ; Humans

Objective To explore the feasibility of the application of autoregressive integrated moving average(ARIMA) model to forcast the monthly incidence rate of bacillary dysentery in Jianghan District of Wuhan ,and to provide scientific references for prevention and control .Methods SAS 9 .2 software was used to fit the ARIMA model for the monthly incidence rate of bacil‐lary dysentery from 2005 to 2013 .Thereafter ,the fitted model was used to forecast the data from January to June in 2014 ,and was evaluated by comparing the actual incidence .Results The ARIMA(0 ,1 ,1)X(0 ,1 ,1)12 model had a better fitted effect ,it was pre‐dicted that the incidence of bacillary dysentery of Jianghan District of Wuhan City from January to June 2014 would increase slight‐ly ,and the forecast data of bacillary dysentery from January 2005 to December 2013 consisted with the actual situation .Conclusion ARIM A model can be used as short‐term forecasting model to predict the monthly incidence rate of bacillary dysentery in Jianghan District of Wuhan .

Objective To study the clinical characteristics of obstructive sleep apnea-hypopnea syndrome in the aged. Methods Forty-two over 60 patients with obstruction sleep apnea-hypopnea syndrome (OSAHS) diagnosed by polysomnography (PSG) and 42 matched but less than 60 years OSAHS patients were selected, who were admitted to our hospital from February 2003 to December 2005. The clinical presentations and the results of PSG monitoring were analyzed between the two groups. Results The apnea-hypopnea index (AHI) and microarousal index (MAI) in the aged group were significantly lower than that in the younger group (P0.05). The number of severe OSAHS in the aged group was less than in the younger group (P

OBJECTIVE To study the effect of chronic intermittent hypoxia and removal of hypoxia on learning and memory ability in Sprague-Dawley rats and the synaptophysin expression in hippocampus CA3 region.METHODS Experiments were performed on 24 three-month-old male Sprague-Dawley rats,They were randomly assigned to three experimental groups(n=8/group):unhandled control group(UC),chronic intermittent hypoxia group(CIH),and Removal of hypoxia group(RH).Rats in UC group were raised normally,those in CIH group suffered 8H intermittent hypoxia everyday,the duration of experiment was 4 weeks,and those in RH group was treated as same as those in CIH group in the first 4 weeks and were raised normally in the next 4 weeks.The learning and memory ability of rats in each group were assessed with the Morris water maze test at the end of the experiments.The expression of synaptophysin in hippocampus CA3 region was investigated by immunohistochemistry staining and image analysis.RESULTS ①Morris water maze test learning scores(place navigation):From the beginning,the escape latency in CIH rats was significantly longer than that in UC rats and RH rats(P

Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Dimethylamines ; analysis ; Methylamines ; analysis ; Workplace

Objective To investigate the effects of chronic renal failure rabbit serum on proliferation and nuclear factor kappa B (NF-κB) activation of rabbit arterial smooth muscle cells (ASMCs) and to explore the possible mechanism. Methods Rabbit model of chronic renal failure was established by the ligation of renal arterial branches. ASMCs were incubated in the media with various concentrations of chronic renal failure serum cultured in vitro. Cell proliferation was assessed by MTT. Cell apoptosis was detected by Hoechst33342 staining. NF-κB p65 nuclear translocation was analyzed by immunofluorescence. Expression of proliferating cell nuclear antigen (PCNA) and NF-κB p65 proteins in response to chronic renal failure serum in ASMCs was determined by Western blotting. Results Lower concentrations of chronic renal failure serum (≤ 10%) could significantly promot the proliferation of ASMCs in a dose- and time-dependent manner. Higher concentrations of chronic renal failure serum (＞10%) could significantly inhibit the proliferation and induce apoptosis of ASMCs compared to the normal control (P＜0.05). Under the stimulation of lower concentrations of chronic renal failure serum, the expression of PCNA and NF-κB p65 increased significantly compared to the normal control (P＜0.01), while decreased markedly under the stimulation of higher concentrations of chronic renal failure serum compared to the normal control (P＜0.01). Under the stimulation of 10% chronic renal failure serum, nuclear translocation of NF-κB p65 in ASMCs was found. Conclusions Different concentrations of chronic renal failure rabbit serum can effectively induce ASMCs proliferation or apoptosis. The mechanism of promoting proliferation may be mediated by activating NF-κB, which will be useful for the treatment of accelerated atherosclerosis in chronic renal failure.

Animals ; Ciliary Neurotrophic Factor ; genetics ; metabolism ; Microwaves ; RNA, Messenger ; genetics ; Rabbits ; Retina ; metabolism ; radiation effects

Objectives:This study was designed to establish the modified method of cardiomyocytes culture in human fetal hearts. Methods:The human fetal heart cells of ventricular muscle were isolated by 0.2% trypsin and 0.1% collagenase and cultured primarily and passaged in Iscove's modified Dulbecco's medium(IMDM) in vitro by means of differential attachment technique.The changes in morphology,ultrastructure,viability,immunocytochemistry antibody staining and immunofluorescence antibody staining of human fetal heart cells were studied in culture. Results:The ratio of viable cells was 99% identified by trypan blue staining.The ratio of attachment cells was 95% after 24 h in culture.The cultured human fetal heart cells were roundness shaped,rod shaped,shuttle shaped,ellipse shaped,star shaped and bifurcate shaped with spontaneous contractility.The myocardial actin and myoglobin are identified in the cultured cells by immunocytochemistry antibody staining and immunofluorescence antibody staining.The ultrastructure of cells was similar to that of the cardiac tissue in vivo by electron microscopy.Human fetal heart cells after 20 d of primary culturing and after 5 d of passaged culturing were growed well. Conclusions:The method for isolating and culturing human fetal heart cells is successful and reliable.This model provides an effective experimental mothod for studying the mechanism of myocardial injury.

Objective To assess the effects of p27mt gene transfection on the proliferation and apoptosis of human hepatocellular carcinoma cell (HCC) lines SMMC-7721. Methods A replication deficient adenovirus vector encoding p27mt (Ad-p27mt) was used and p27mt cDNA was transfected into human SMMC-7721 cell lines in vitro. The synthesis of DNA in SMMC-7721 cells was determined by using 3H-thymidine incorporation; the cell apoptosis was determined by flow cytometry, TUNEL method and DNA fragmentation analysis. Results The virus titer was 7.95?1012 cfu/ml, the transduction efficiency was 100 % when multiplicity of infection ≥50, FCM analysis revealed a sub-G1 cell peak in Ad-p27mt transduced hepatocellular carcinoma cell lines. Agarose electrophoresis showed marked ladder .The difference of apoptotic index between the Ad-p27mt group and the control group was statistically significant (58.6?4.3, vs 4.5?1.6, P