Objective]To summarize professor and national physician master Guo Chengjie asarum clinical application experience, to do good to the practitioners. [Methods] Through analyzing Guo Lao clinical experience of the medicinal nature of the composition and toxicity of understanding, to adapt to the conditions and the usage and dosage of the original understanding, analyse and summarize the experience of clinical application of asarum. [Results] Professor Guo Chengjie applies asarum poliomyelitis to cold organs and blockage for the treatment of diseases such as lung;Drug compatibility exalts development, and expands the application in the mammary gland disease by asarum;On the dosage of asarum characteristics and decoction methods give unique insights;Guo Lao especially emphasizes the importance of dialectical medication. [Conclusion] Pro. Guo's application experience and innovation, asarum clinical curative effect is good, is worth using for reference.

Objective To investigate the expression of Oct-4 as a stem cell marker in gastric carcinoma tissues and its clinical significance. Methods From June 1996 to March 2006, 63 paraffin samples of gastric carcinoma tissues were obtained, and the expression of Oct-4 was examined by immunohistochemical staining. Another 58 normal tissues adjacent to gastric carcinoma and 10 normal gastric mucosa tissues were served as controls. The clinieopathologieal data of 63 patients with gastric carcinoma were retrospectively analysed, and their relationship with the expression of Oct-4 was analysed. Fifty of these 63 patients were followed up for 8 years, Kaplan-Meier method and Log-rank teat were employed to explore the correlation between Oct-4 expression and survival, and Cox Regression analysis was performed to evaluate the possibility of Oct-4 expression as an independent prognostic factor for gastric carcinoma. Results The positive expression rate of Oct-4 in gastric carcinoma tissues was 80.95% (51/63), significantly higher than that of normal tissues adjacent to gastric carcinoma (5.2%, 3/58) and normal gastric mucosa tissues (0) (P < 0.01). The expression of Oct-4 was positively correlated to the clinical stage of gastric carcinoma(P<0.01). There was no significant difference in the expression of Oct-4 among patients with different differentiation, age, and gender (P>0.05). Patients with higher expression of Oct-4 had significantly shorter survival time (P<0.05). The expression of Oct-4 was not significantly correlated with the prognosis of gastric carcinoma(RR, 0.554; 95% CI, 0.209-1.466). Conclusion The expression of Oct-4 is correlated with the clinical stage and survival time of patients with gastric carcinoma, however, it is not an independent prognostic factor for gastric carcinoma.

Biomarkers ; Cerebral Hemorrhage ; etiology ; prevention & control ; Humans ; Infant, Newborn ; Protein Precursors ; Prothrombin ; Vitamin K Deficiency ; complications ; diagnosis ; Vitamin K Deficiency Bleeding ; etiology ; pathology ; prevention & control

Objective To investigate the effect of somatostatin on pancreatic blood flow and pancreatic function on the patients with acute pancreatitis.Methods 120 patients with acute pancreatitis from March 2015 to March 2017 were collected, and were randomly divided into the observation group and the control group.The control group were given routine treatment, at the basis of this, the observation group were given somatostatin.octreotide 0.6 mg was dripped into intravenous continuously, and was reducted to 0.3 mg, continuous treatment for 7 d.The changes of blood flow and the curative effect of pancreas were compared between the two groups before and after treatment.Results The total effective rate was 96.67%(58/60)in the treatment group and 80.00%(48/60)in the control group.The total effective rate in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05).There was no significant difference in blood flow(BF), blood volume(BV)and capillary surface permeability(PS)between the two groups before treatment.1 and 7 days after treatment, BF, BV in the observation group were higher than those in the control group, the levels of PS were significantly lower than that in the control group, the differences were statistically significant(P<0.05).Before treatment, the differences of(CP), insulin(INS), glucagon(GLU), serum lipase(LIP), amylase(AMY)and trypsinogen-2(TPS)were measured in the two groups were not statistically significant.1 and 7 days after treatment, CP and INS in the observation group were significantly higher than those in the control group, and GLU, LIP, AMY and TPS-2 were significantly lower than those in the control group, the differences between the two groups was statistically significant (P<0.05).Conclusion It has a protective effect on pancreatic blood flow and function which somatostatin was used in the treatment of the patients with acute pancreatitis, it can improve the overall state of pancreas, and has significant therapeutic effect.

Adult ; Aged ; Alveolar Ridge Augmentation ; methods ; Dental Implantation ; methods ; Female ; Humans ; Male ; Maxillary Sinus ; surgery ; Middle Aged

OBJECTIVE To investigate the present situation of hand-washing among medical staff then explore more suitable hand-washing methods and monitoring measure. METHODS By surveying the hand-washing situation of 96 medical staff in our hospital through the method of sampling,the different germ eradication rate between routine soap hand-washing and disinfectant wet towel hand-wiping as well as hand-washing rate in all candidate population during practical work were observed. RESULTS The germ eradication rate after routine soap hand-washing was 86.0%,while by disinfectant wet towel wiping it was increased to 91.3%.The washing rate after above former method was 18.3% and after above two methods combined was increased to 43.6%,and after health propaganding and training was up to 60.6%. CONCLUSIONS Disinfectant wet towel hand-wiping is convenient,practical and effective which would be more suitable for clinical practice when combined with routine soap washing method.

Objective To study the value of laparoscopic cholecystectomy (LC) for treating gallbladder benign diseases in aged patients. Methods Laparoscopy cholecystectomy (3-port or 4-port) was performed in 2335 senile patients with gallbladder benign diseases from December 1992 to May 2005. Results Five patients were found as having the Mirizzi syndrome and were given a subtotal cholecystectomy. In 18 patients with a frozen Callot’s triangle, the anterior wall of the gallbladder was resected and the mucous membrane on the posterior wall was electrocauterized. LC was completed smoothly in the remaining 2312 patients. No conversions to open surgery were needed. The operation time was 8~55 min (mean, 19 min), and the intraoperative blood loss was 1~ 50 ml (mean, 8 ml). An abdominal drainage tube was placed in 213 patients, including double-cannula lavage in 56 patients. The length of hospital stay was 4~16 d (mean, 6 d). Follow-up examinations for 10 days ~13 years in 1859 patients showed no biliary stenosis, gallbladder pouch, or residual stones. Conclusions Laparoscopic cholecystectomy is safe and feasible in aged patients, being the first choice of operative method. Proper perioperative management, careful prevention co-morbidities, and control of operation time as short as possible are important for senile patients.

Objective To study the characteristics of maxillary growth after lip and palate repair. Methods Using X-ray cephalometry through 12 landmarks and 12 measured values we had compared the difference between 60 patients with operated cleft lip and palate and 20 normal persons. Results After the operation all patients appeared limits of maxillary growth in different degree and different way. SNA and N-A-Pg [(-4.8?6.31)mm, P 0.05] were smaller than normal in patients of cleft lip. SNA(74.5?4.01)?, P 0.05;(75.1?1.07)?, P 0.01], and N-ANS [(47.3?2.41)mm, P 0.01;(49.8? 1.91) mm, P 0.01] were also smaller than normal in cleft palate and cleft lip and palate. Conclusions The limits of maxillary growth are affected by congenital and operative factors. Lip repair will influence the length of maxilla, and palate repair will influence height and width of maxilla.

12 ml/s was used as Group A and Qmax ≤12 ml/s as Group B. Results One week after removal of urinary catheter, dysury appeared in five cases with Qmax for (9.45?2.62) ml/s, of which two cases were with posterior urethral stricture, two with benign prostatic hyperplasia and one with weak detrusor contraction. Three months after removal of urinary catheter, six cases led to secondary dysuresia due to posterior urethral stricture and had Qmax for (6.28?3.26) ml/s, with significant difference compared with urodynamic parameters of normal group (P

Objective To set up a RP-HPLC method for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously. Methods ODS-2 Hypersil column was used with methanol-1% glacial acetic acid as solution gradient elution. The flow rate was 1 mL/min and column temperature was 25 ℃. The wavelength of detector of gastrodin was 270 nm, and ferulic acid was 322 nm. Results Gastrodin and ferulic acid can be separated well with other components within 40 minutes. The linear range of gastrodin was 0.092～1.840 ?g (r =1.000 0), and ferulic acid was 0.122～2.440 ?g (r =1.000 0). The average recovery rate of gastrodin was 99.50% with RSD=1.23% (n =5), and ferulic acid was 101.5% with RSD=1.52% (n=5). Conclusion The method is simple, rapid, accurate and reliable, and can be used for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously.