Humans ; Laryngeal Cartilages ; pathology ; Laryngeal Neoplasms ; diagnosis ; pathology ; Male ; Middle Aged ; Necrosis ; Neoplasm Recurrence, Local ; diagnosis ; Radiation Injuries ; diagnosis ; pathology

ObjectiveTo compare the effects of two methods for the treatment of delayed defecation of neonatal meconium. Methods Sixty-seven neonates with delayed defecation of neonatal meconium were divided into experiment group (n=37) and control group (n=30). The former group was managed with abdominal massage followed by glycerol enema and the latter with abdominal massage followed by anus stimulation.Then the groups were compared in terms of the time for initial defecation of meconium,the volume of defecation,exhanstion time for defecation,abdominal distention and vomiting and serum bilirubin within 7 days.Result The treatment group was superior to the control one in terms of time for initial defecation of meconium,the volume of defecation, exhanstion time for defecation (P<0.05) and the incidences of abdominal distention,vomiting and serum bilirubin within 7 days were significantly lower than the control group (P<0.05).Conclusions The method of glycerol enema combined with abdominal massage is more effective for promoting defecation of neonatal meconium than the method of anus stimulation.It can reduce the incidence of abdominal distension,vomiting and pathological jaundice.

OBJECTIVETo study the effect of hepatitis C virus nonstructural protein 3 N-terminal protein (HCV NS3-5') on hepatocyte transformation and tumor development.

METHODSQSG7701 cells were transfected with plasmid pRcHCNS3-5' (expressing HCV NS3 N-terminal protein) by lipofectamine and selected in G418. The expression of HCV NS3 gene and protein was determined by PCR and immunohistochemistry respectively. Biological effect of transfected cells was observed through cell proliferation assay, anchor independent growth, and tumor development in nude mice. The expression of HCV NS3 and c-myc protein in the induced tumor was evaluated by immunohistochemistry.

RESULTSHCV NS3 was strongly expressed in QSG7701 cells transfected with plasmid pRcHCNS3-5' and the positive signal was located in cytoplasm. The HCV NS3 expression and c-myc protein in the induced cytoplasm. Cell proliferation assay showed that the population doubling time in the pRcHCNS3-5' transfected cells was much shorter than that in the pRcCMV and non-transfected cells (24 h, 26 h, 28 h respectively). The cloning efficiencies of transfected cells with pRcHCNS3-5', pRcCMV and non-transfected cells were 33.0%, 1.5%, 1.1% respectively (P < 0.01). Tumor developed in nude mice inoculated with pRcHCNS3-5'transfected cells 15 days after the inoculation. HE staining showed hepatocarcinoma character and immunohistochemistry confirmed HCV NS3 and c-myc expression in the tumor tissue. The positive control group also showed tumor development, while no tumor mass obtained in the nude mice inoculated with pRcCMV and non-transfected cells even 40 days after the injection.

CONCLUSIONHCV NS3 N-terminal protein showed cell transformation and tumorigenic features.

Animals ; Cell Division ; Cell Transformation, Neoplastic ; Female ; Hepatocytes ; pathology ; Humans ; Liver Neoplasms, Experimental ; etiology ; Mice ; Mice, Nude ; Mitogen-Activated Protein Kinases ; metabolism ; Phosphorylation ; Transfection ; Viral Nonstructural Proteins ; toxicity

OBJECTIVETo investigate the changes in electromyographic activities of the temporal and masseter muscles at different positions of the mandible.

METHODSTwenty orthodontic patients with Angle Class II malocclusion and mandibular retrusion (ANB<6°) aged 10-14 years were enrolled in this study. All the patients were treated with Forsus fixed functional appliance combined with MBT straight-wire appliance. The electromyographic activities of the temporal (T) and masseter (M) muscles before, during and after functional treatment were evaluated by assessing the average integrated electromyogram (EMG) and T/M ratio at clenching status in different mandibular positions.

RESULTSAfter functional forward positioning of the mandible, the electromyographic activities of the temporal and masseter muscles decreased and T/M ratio increased significance at the clenching status (P<0.05).

CONCLUSIONThe appliance insertion and activation is associated with a decreased EMG activity of the temporal and masseter muscles, and the T/M ratio is correlated to the position of the mandible.

Adolescent ; Child ; Electromyography ; Female ; Humans ; Male ; Malocclusion, Angle Class II ; physiopathology ; therapy ; Masseter Muscle ; physiopathology ; Orthodontic Appliances ; Retrognathia ; physiopathology ; Temporal Muscle ; physiopathology

Objective To study the effect of operating rooms of two different cleanliness on surgical site infection (SSI)in neurosurgical craniocerebral operation,provide reference for the prevention and control of SSI.Methods Neurosurgical craniocerebral operation undertaking in grade I and II operating rooms in a hospital between July 2015 and July 2016 was performed retrospective study,patients in grade I operating rooms were as control group and those in grade II operating rooms were as trial group,occurrence of SSI was compared between two groups of pa-tients.Results A total of 454 patients undergoing craniocerebral operation were investigated,212 patients were in control group (grade I operating rooms)and 242 in trial group (grade II operating rooms).Patients in control group had 25 cases of SSI,incidence of SSI was 11.79%;trial group had 31 cases of SSI,incidence of SSI was 12.81%. Nine cases of SSI were superficial incision infection,there was no deep incision infection,the other 47 cases of SSI were intracranial infection(organ space infection).There was no significant difference in the incidence of SSI be-tween two groups of patients(P＞0.05).Conclusion Grade I and II cleanliness operating rooms have no direct effect on SSI in patients undergoing neurosurgical craniocerebral operation,comprehensive prevention and control mea-sures should be taken to prevent the occurrence of SSI.

Objective To analyze the protective effects of different incision protection styles on incision bacterial growth of cesarean.Methods Totals of 300 patients of cesarean section were divided into three groups randomly with 100 patients in each group:group one which operation film was pasted after operation sheet draped,the operation film was pasted before operation sheet draped in group two,and traditional skin towel was applied in group three.Subcutaneous tissue fluid was dipped after skin incision and before skin suture in each patient.The tissue fluid was bacterial cultured,and the influence of different incision protection styles on incision infection in cesarean was judged according to the bacterial growth.Results The preoperational tissue fluid had no bacterial growth in group one,group two and group three (x2 =1.31,P ＞0.05).The postoperative tissue fluid had zero bacterial growth in group one,and one in group two,and seven in group three.The bacterial growth rate in group three was higher than that in group one and group two (11.67％ vs 0％ and 1.67％) (x2 =4.69,P ＜ 0.05),but there was no significant difference between group one and group two (P ＞0.05).Conclusions The operation film pasted after operation sheet drape may be more effective on prevention of incision bacterial growth in cesarean section.

This study was aimed to clarify whether valproic acid (VPA) induces apoptosis of leukemia HL-60 cell line and its possible mechanism. The effect of different concentrations and treatment time of VPA on HL-60 cell proliferation was assayed by cytotoxicity test (CCK-8 method) and fluorescence microscopy, and flow cytometry was used to detect cell apoptosis. The expressions of telomerase subunit h-tert mRNA and apoptosis-related protein as well as caspase-3 activity were detected by real time-quantitative PCR, Western blot and ELISA respectively. The results indicated that VPA inhibited proliferation of HL-60 cells and induced cell apoptosis in a dose dependent manner (r = -0.87). The expressions of anti-apoptotic protein BCL-2 and h-tert mRNA were significantly decreased while the pro-apoptotic protein BAX and caspase-3 activity increased after treatment with VPA. The apoptosis rate of HL-60 cell was negatively correlated with expression of h-tert mRNA. It is concluded that VPA can inhibit leukemia HL-60 cell proliferation and induce apoptosis. The VPA displays anti-leukemia activity possibly through reducing h-tert mRNA and BCL-2 protein expression, increasing BAX expression and activity of caspase-3.
Apoptosis ; drug effects ; Caspase 3 ; metabolism ; HL-60 Cells ; Humans ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Telomerase ; metabolism ; Valproic Acid ; pharmacology ; bcl-2-Associated X Protein ; metabolism

Objective The aim of the study is to evaluate the left ventricular(LV)dyssynchrony in chronic heart failure(HF)Patients with normal and wide QRS duration.Methods Time to peak velocity at peak systolic and early diastolic phase(Ts and Te)were determined in 12 segments of LV by echocardiography(GE Vivid 7)in 54 HF patients(28 with wide and 26 with normal QRS duration)and 15 normal controls to evaluate LV systolic and diastolic dyssynchrony.The risk factors related to LV dyssynchrony were also evaluated.Results LV end systolic and diastolic volumes were significantly larger and 12 segmental mean Ts and maximal Te difference(Te-diff)were significantly higher in HF patients with wide QRS duration tllan HF patients with normal QRS duration.Using mean Ts≥182 ms as the cut-off value,systolic dyssynchrony was present in 46% HF patients with normal QRS and 71% HF patients with wide QRS.Using Te-diff≥79 ms as the cut-off value,diastolic dyssynehrony was seen in 58% HF patients with normal QRS and 89% HF patients with wide QRS.Combined systolic and diastolic dyssynchrony was seen in 31% HF patients with normal QRS and in 64% HF patients with wide QRS.Systolic dyssynchrony Was significantly correlated to LV end systolic volume and diastolic dyssynchrony was correlated to end diastolic volume.Conclusion Percentage of LV dyssyncrony was significantly higher in HF patients with wide QRS,especially in HF patients with increased LV end systolic and diastolic volume.

OBJECTIVETo study the gene characterization of enterovirus 71 (EV71) virus strains isolated from clinical specimens of children with hand-foot-and-mouth disease (HFMD) in Zhejiang province.

METHODSVirus were isolated from clinical samples including stool, throat swab and vesicle from patients with HFMD. The EV71 isolates were identified by microneutralization assay and reverse transcriptase PCR (RT-PCR) with specific primer pair for VP1 genes of EV71. Complete VP1 gene sequences (891 nucleotides) for recent 6 EV71 isolates were determined and compared with that of A, B, C genotype reference EV71 strains and 11 EV71 China isolates available from GeneBank by homogeneity and phylogenetic tree analyses.

RESULTS9 strains of EV were isolated from 14 clinical specimens. Data from microneutralization and RT-PCR results indicated that all the strains belong to EV71. The nucleotide and amino acid homogeneity of these 6 Zhejiang strains with the representative isolates of A and B genotypes were 82.9%-85.5% and 94.9%-98.0% respectively; with the representative isolates of C were 89.2%-94.1% and 97.0%-99.0% respectively. There were 91.0%-92.2%, 90.2%-90.3%, 89.2%-89.5%, 96.7%-96.9% nucleotide, homology with representative strains of C1, C2, C3,C4 subgenotypes of EV71. The nucleotide homogeneity of these 6 EV71 isolated strains with 9 previously isolated Chinese strains appeared to be 93.8%-97.1%. These 6 EV71 isolated strains were within genotype C subgenogroup C4 in the phylogenetic tree.

CONCLUSIONThe recently identified EV71 isolates in Zhejiang province belonged to subgenogroup C4.

Child ; China ; Enterovirus ; classification ; genetics ; isolation & purification ; Genes, Viral ; Genotype ; Hand, Foot and Mouth Disease ; virology ; Humans ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid

OBJECTIVETo investigate the social mental state of drug addicts in a compulsive drug abuse treatment center; evaluate the effectiveness of integrated program for the prevention of abuse relapse and improvement of drug addicts' psychological health.

METHODSThe study subjects were addicts from the Wuhan Compulsive Drug Abuse Treatment Center between October 2003 and June 2004, who satisfied the inclusion criteria. A non-randomized control-intervention study design was adopted. Volunteers willing to take part in intervention were put into the intervention group with their full awareness and willingness to prevent drug abuse relapse. The control group was composed of the addicts who were willing to prevent relapse and to be followed up after their discharge.

RESULTSThe effectiveness of the integrated intervention program in promoting addicts' psychological health: before the intervention, the scores of Self-Rating Anxiety Scale (SAS), the positive and negative dimensionalities of Simple Coping Style Questionnaire (SCSQ) and Chinese Perceived Stress Scales (CPSS) had no significant differences between the intervention group and the control group. After the intervention, except that the SCSQ's positive dimensionality in the intervention group was significantly higher than that in the control group, other indices in the intervention group were lower. Before and after the intervention, the psychological health level in both the groups was lower than that in the normal population; there were significant differences between addicts and normal subjects in regards with all of the indices above.

CONCLUSIONDrug abuse was associated closely with addicts' social mental factors. The integrated intervention program can alleviate anxiety and stress, reduce co-morbid mental disorders and effectively improve their coping style. In conclusion, the program can promote addicts' psychological health significantly.

Adaptation, Psychological ; Adult ; Case-Control Studies ; Female ; Humans ; Male ; Mental Health ; Program Evaluation ; Stress, Psychological ; Substance-Related Disorders ; psychology ; therapy