1.Causes and treatment of gastrointestinal complications following anterior approach to thoracolumbar fracture and dislocation
Chinese Journal of Trauma 1993;0(06):-
Objective To explore the causes and treatment of gastrointestinal complications after anterior approach for thoracolumbar fracture and dislocation.Methods A retrospective analysis was carried out in 153 cases with thoracolumbar fracture and dislocation undergone anterior approach from Jan- uary 1999 to October 2003.Postoperative gastrointestinal complication was seen in 15 cases including sev- en with dynamic intestinal obstructions,three with stress ulcer,three with intestinal bacteria imbalance, one with tuberculosis peritonitis resulted from dissemination of primary tuberculosis,and one with acute relapse of chronic appendicitis.Results All patients were effectively cured by using corresponding methods in regard of different causes.Conclusions(1)Gastrointestinal complications following ante- rior approach for thoracolumbar fracture and dislocation are mainly resulted from following causes,ie,se- rious primary trauma,primary gastrointestinal vegetative nerve injury or that caused by surgical operation, intraoperative contusions of abdominal viscera,postoperative retroperitoneal hematoma,acute lesion of gastric mucous membrane as well as imbalance of intestinal flora.(2)Correct treatment of primary trau- ma,meticulous operation,reasonable utility of antibiotics and appropriate management can effectively prevent and control gastrointestinal complications.
2.Analysis of early complications after using Vertex technique in treating Dens axis fracture with atlantoaxial dislocation
Jianping KANG ; Qing WANG ; Daxiong FENG
Orthopedic Journal of China 2006;0(10):-
[Objective]To discuss the causes and preventive measures of early complications after using Vertes technique in treating dens axis fracture with atlantoaxial dislocation.[Method]Eighteen cases performed Vertex technique were included in this study.The early complications(postoperative or at surgery) included vertebral artery injury(2 cases),logagnosia(1 case),anopsia(1 case),hematorrhea(1 case) and wrong instrumentation(1 case).The causes of these complications and how to prevent were analyzed.[Result]All these complications were treated in time,and no sequela was found.[Conclusion]The early complications of Vertex technique in treating dens axis fracture with atlantoaxial joint dislocation can be prevented.The timely treatment must be performed when it occurs,and the effect would be satisfactory.
3.Vertex technique for treatment of atlantoaxial instability:Clinical outcomes and implant features
Jianping KANG ; Daxiong FENG ; Qing WANG
Chinese Journal of Tissue Engineering Research 2007;0(01):-
This paper is aimed to explore the correlation of clinical outcomes with material science features of implant in the treatment of atlantoaxial instability by the Vertex technique.A total of 26 cases with atlantoaxial instability and dislocation were enrolled from the Affiliated Hospital of Luzhou Medical College between January 2004 and January 2006.They all underwent the internal fixation via posterior cervical pathway and atlantoaxial fusion in Vertex system that was produced by American Sofamor Danek Company.The intraoperative complications and postoperative neurological recovery of each case were documented.Dynamic radiograph and digital tomography were used to evaluate the extent of the atlantoaxial vertebrae stability.①The result of X-ray showed that bony fusion was successful in 22 patients during 3-month follow-ups,3 patients during 6-month follow-ups,and 1 case after implantation following bony resorption.②There was no deterioration of spinal cord injury and the JOA scores of neurofunction increased from 3-17 points(mean 6.8 points) to 5-17 points(mean 14.8 points) ,with the rate of improvement as 83%.③All 26 patients were normal in the movements of expansion and flexion,but the rotation was partly limited.There were 18 cases rotate in 120?,6 cases in 100? and 1 case in 60?.Vertex system is effective in dealing with internal fixation via posterior cervical pathway.Due to the connection with stick by multiaxial screw,Vertex system presents superiority in clinic,such as secure fixation,random length and convenient connection.Vertex system internal fixation is effective in dealing with atlantoaxial instability and dislocation.
5.A study on the anesthesiologist deployment at tertiary hospitals
Lian DUAN ; Jingtong WANG ; Qing QIAO ; Yi FENG ; Shan WANG
Chinese Journal of Hospital Administration 2014;30(12):926-929
Objective To learn the current anesthesiologists deployment at the hospital,and to figure out how to measure anesthesiologists deployment.Methods Existing data and expert consultation were used to obtain various indicators,and the current anesthesiologists deployment was analyzed,calculating with the formula the needed number of anesthesiologists.Results Despite the rising demand and resource utilization,anesthesiologists were found in obvious shortage at the hospital.The number of anesthesiologists needed was calculated as 101.4,with a vacancy of 11.4.Conclusion The national health authorities were recommended to revise the standard of anesthesiologists deployment at hospitals.
6.Advance in the study of targeting delivery system for siRNA mediated by aptamers.
Xiao-Lin WANG ; Qing-Qing WANG ; Hai-Feng SONG
Acta Pharmaceutica Sinica 2012;47(7):850-855
RNA interference (RNAi), as a new technology of gene therapy, has been used in the studies of many diseases in vitro, however, targeting delivery of small interference RNA (siRNA) is still a bottleneck for clinical therapy of siRNA agents. Aptamer is a group of oligonucleotides with high affinity and targeting, and is becoming another important means of delivery for siRNA. In this review, we summarized siRNA delivery obstacles in vivo and recent attractive developments increatively using cell-internalizing aptamers to deliver siRNAs to target cells.
Aptamers, Nucleotide
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administration & dosage
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metabolism
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Drug Delivery Systems
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methods
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Humans
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Neoplasms
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therapy
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RNA Interference
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RNA, Small Interfering
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administration & dosage
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metabolism
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SELEX Aptamer Technique
7.Progress in the treatment of diabetic wound healing via stem cells transplant.
Qing-xin CAI ; Lu WANG ; Yan ZHANG ; Bao-feng YANG
Acta Pharmaceutica Sinica 2015;50(1):1-6
The morbidity of diabetes has been increasing rapidly in recent years. Delayed wound healing has become a common complication in diabetes, which seriously affects the orthobiosis of patients. Exploring and finding the molecular mechanisms of diabetic wound healing and the effective therapies to promote wound healing have important clinical significances. Stem cells transplant has become a research hotspot in accelerating diabetic wound healing. This article reviewed the present approaches concerning stem cells transplant in diabetic wound healing both at domestic and abroad, and looked forward the clinical therapy of stem cells on diabetic wound healing.
Diabetes Mellitus
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therapy
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Humans
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Stem Cell Transplantation
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Stem Cells
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Wound Healing
8.Imaging Diagnosis of Round Atelectasis
Xili ZHAO ; Jian FENG ; Qing ZHOU ; Zhixue WANG
Journal of Practical Radiology 2000;0(02):-
Objective To study the imaging manifestati ons and diagnostic evaluation of rounded atelectasis.Methods Imaging manifestation of round atelectasis in 4 c ases confirmed by pathology with complete clinical data,plain chest radiographs and CT were analysed.Results The lesions in 4 cases appeared as circular or el liptic masses with clear boundary,2.6~6.5 cm in diameter,located at subpleura o f lower lobule of lung,accompanied with pleural thickening.There was a acute angl e between the tumor and thickened pleura.The lucency of the lung adjacent to tum or incneased.The cordike structures consisted of blood vessels and bronchil ente red into tumors,which made the lateral boundary of tumor to hilum of lung obscur ed.“comet tail sign”and “air-brochogram sign”were seen in 3 cases.Conclusion The round atelectasis is of typical findings o n plain chest films and CT scans which play a very important role in diagnosis a nd differential diagnosis.
9. Isolation and expression analysis of RgMed6 from Rehmannia glutinosa
Chinese Traditional and Herbal Drugs 2015;46(19):2919-2924
Objective: To clone RgMed6 gene, which coded a subunit of mediator complex, from Rehmannia glutinosa, and to analyze the characteristics of protein sequence and gene expression. Methods: The transcriptional EST database of R. glutinosa was used to search analogs of AtMed6 gene by BLAST, the full-length open reading frame (ORF) of RgMed6 was obtained by assembling the ESTs. BLASTp, the online analysis tool of NCBI was used to get the homologous sequences of RgMed6, and MAFFT has been performed to analyze the multiple sequence alignment. Phylogenetic tree has been constructed using MEGA 6.0 software. Quantitative RT-PCR has been applied to detecting the transcription level of RgMed6 in five tissues as well as in tuberous roots or leaves under three stresses. Results: The cDNA sequence of RgMed6 containing 924 bp was obtained. The ORF of RgMed6 was 771 bp encoding 256 amino acids, which had typical structural domains and a potential nuclear localization signal (NLS). RgMed6 showed the highest expression level in leaves, followed by buds, but very weak in stems. The transcription level of RgMed6 mRNA was reduced under continuous cropping conditions in tuberous roots while it increased under salinity stress in leaves. Conclusion: RgMed6, a mediator subunit gene from R. glutinosa has been obtained for the first time, which can lay the foundation for further studies about its molecular function in development and responses to stress.
10.Observation of experimental corneal neovascularization induced by alkali burn
lei, ZUO ; xun, XU ; ying, FAN ; feng, WANG ; qing, GU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(06):-
Objective To induce the experimental corneal neovascularization(CRNV) by alkali burn,and explore the methods for quantitative analysis of CRNV and observation of permeability of CRNV. Methods Thirty-six C57BL/6 mice were selected and divided into experiment group(n=30) and control group(n=6).For the experiment group,alkali burn was induced by application of filter paper with 1mol/L sodium hydroxide to the cornea for 5 s.For the control group,no intervention was conducted.Areas of CRNV were measured on day 4,7,10 and 14 after alkali burn.Histological examinations of cornea were performed with HE staining on day 3, 7,10,16 and 28 after alkali burn.On day 10,endothelial cell marker CD31 was used with immunohistochemical staining for CRNV counting,and fluorescence angiography(FA) was employed to reveal the permeability of CRNV.Corneal ulceration and hyphema were observed everyday.Results CRNV developed after alkali burn,and extincted afterwards.Axenic coneal ulceration and hyphema were frequently observed,with the incidences of 6.7% and 10.0%,respectively.Histologic changes of corneal tissues at different time points could be observed with HE staining.On day 10, CRNV could be labeled and counted with immunohistochemical staining for CD31 antibody,and the permeability of CRNV could be detected by FA. ConclusionCRNV counting with immunohistochemical staining for CD31 antibody and measurement of area of CRNV are appropriate methods for quantitative analysis of CRNV.FA is an effective method in the detection of permeability of CRNV.