Objective To analyze the blood of patients with hepatitis B cirrhosis complicated with iron deficiency anemia(IDA).Methods The 100 cases with hepatitis B cirrhosis complicated with IDA and hepatitis B cirrhosis complicated with non-iron deficiency anemia (NIDA) were recruited in our hospital from December 2013 to May 2015,and were divided into observation group (50 cases) and control group(50 cases) according to whether complicated with IDA.The liver function,blood routine,liver cirrhosis patients with coagulation test and measurement of the platelet parameters of two groups of patients were detected and analyzed.Results After treatment,no statistically significant difference were observed the levels of albumin,bilirubin,bile acid and alkaline phosphatase between two groups (P>0.05).The levels of alanine amino transferase (ALT),gamma glutamyltransferase (GGT) in the observation group were significantly higher than those in the control group(P<0.05).The hemoglobin,serum ferritin (SF),mean corpuscular volume (MCV),mean corpuscular hemoglobin (MCH) values in the observation group were lower than those in the control group,while level of folic acid was significantly higher than that of the control group(P<0.05).There was no significant difference on the red blood cell count (RBC) between two groups (P>0.05).The prothrombin time (PT),International normalized ratio (INR),activated partial thromboplastin time (APTT),thrombin time (TT) of observation group were higher than the control group(P<0.05);while the levels of fibrinogen (FIB),platelet count (Plt) were significantly lower than those of control group(P<0.05).There was no significant difference in platelet distribution width (PDW) between the two groups (P>0.05).Conclusion The detection of blood in patients with hepatitis B cirrhosis complicated with IDA is of great significance in the evaluation of the degree of liver damage and the diagnosis of the disease,which is worthy of clinical application in the future.

AIM:To investigate the roles of maspin in the biological behaviors of non-small-cell lung cancer A549 cells.METHODS:Full-length coding region of maspin was amplified by PCR from human normal breast tissue and cloned into MSCV vector .Virus supernatants was produced by Phoenix A packaging system , and target cell line was infec-ted with the virus supernatants .The transfected cells were screened with puromycin .The cell line with maspin over-expres-sion was identified by real-time PCR and Western blot.Cell growth, migration and invasion were investigated by xCelli-gence system .RESULTS:Maspin over-expression vector was successfully constructed .The cell line with maspin over-ex-pression was established .No difference of the growth between A 549-control cells and A549-maspin cells was observed .The migration and invasion were quite different between A 549-control cells and A549-maspin cells.The expression level of inte-grin β1 in A549-maspin cells was decreased compared with A 549-control cells .CONCLUSION:Maspin has no effect on the growth of A549 cells.Maspin suppresses the migration and invasion of A 549 cells, in which integrin β1 is involved.

Child ; Humans ; Proteomics ; methods

Objective To determine levels of nuclear factor (NF)-κB, interleukin (IL)-10, and visfatin in adipocytes treated by different degrees of intermittent hypoxia (IH), and to investigate the mechanism of IH leading to insulin resistance (IR). Methods The cell model of intermittent hypoxia/re-oxygenation (IH/ROX) in obstructive sleep apnea (OSA) was established. Differentiation mature 3T3-L1 adipocytes, were randomly divided into 10 groups including four different-frequency intermittent hypoxia groups(IH1-4, fixed intermittent hypoxia scheme for 1.5%O2 45 s and then re-oxygen 21%O2 for 2 min 15 s, 4 min 15 s, 5 min 45 s and 8 min 45 s, 60 times circulation), and their normal oxygen control groups (SC1-4, instead each IH group 1.5%O2 to 21%O2, the rest groups were treated as same as IH group), continuous hypoxia group (CH, 10%O2 for 6 h) and normal oxygen control group (CC, 21%O2 for 6 h). ELISA method was used to determine the levels of IL-10 and visfatin in the supematant of adipocytes. Western blot method was used to determine the protein levels of NF-κB p65 and visfatin. Real-time PCR method was used to determine the mRNA levels of IL-10 and visfatin. Results The protein and mRNA expressions of IL-10 were significantly lower in IH group and CH group than those of control groups (P<0.01). The levels of NF-κB p65 protein were significantly increased in IH group and CH group than those of control group. The protein and mRNA expressions of visfatin were significantly higher in IH1, IH2 and CH groups than those of control group (P<0.01). Conclusion As a prominent feature of OSA pathophysiology, IH may take part in insulin resistance of OSA patients by abnormally secreting NF-κB, IL-10 and visfatin in adipocytes.

In order to meet the education reform and the needs of diversified society,according to the talents training goal of generic eagle plan of Inspection Department of Chongqing Medical University,this article use the pin type education management to awake the student's potential advantage,cultivate a variety of laboratory medicine talented person.This article was focused on pin type education management scheme,implementation methods,results and experience.

Adult ; Empyema, Pleural ; complications ; etiology ; Humans ; Male ; Neck Injuries ; complications ; Tracheoesophageal Fistula ; complications ; etiology

OBJECTIVE To approach the distribution characteristic and drug resistance status in pathogens from infected urinary tract in Jingshan County and offer the scientific evidence for clinically reasonable use of antibiotics.METHODS Totally 218 strains of pathogens isolated from urinary tract were identified by routine methods according to the National Clinical Laboratory Operation Rules.Drug susceptibility testing was performed by K-B methods recommended by CLSI.RESULTS Among 218 strains of pathogens from infected urinary tract,Escherichia coli accounted for 61.0%,ranking the first and enterococci ranked the second,accounted for 11.5%.Except for 100% sensitivity to vancomycin and teicoplanin in Gram-positive bacteria and to imipenem and meropenem in Gram-negative bacteria,the pathogens from infected urinary tract had been resistant to commonly used antibiotics in some degrees.The detection rate of extended-spectrum beta-lactamases(ESBLs) producers was 32.9%.CONCLUSIONS The drug resistance in pathogens from infected urinary tract is on the rise.We should strengthen monitoring and controlling of it.

Objective To analyze the changes of P wave of intracavitary electrocardiogram (ECC) in the placement of periph erally inserted central venous catheters (PICC), and to explore whether the intracavitary ECG can be used to guide the procedure. Methods PICC was inserted in 62 adult patients under intracavitary ECC-guided technique. The intracavitary ECC were recorded in different parts of vessel access through micro-guidewire. Results The intracavitary ECG extracted through micro-guidewire were accurately recorded in all patients. The amplitudes of P wave were (1.11 ±0.36)mm, (1.12±0.28)mm,(1.56±0.45)mm, (4.03±1.87)mm, (5.90±2.45)mm, (7.90±2.95)mm, (9.87±2.77)mm, (5.00±1.76)mm, (2.50±1.73)mm when PICC tip was located in the axillary, subclavian and brachiocephalic vein, in the superior, middle and inferior SVC, at the SVC-right atrium junction, and in the middle, inferior right atrium,respectively. The amplitude of P wave was 1.14±0.34mm in 10 patients with PICC tip malposition in the internal jugular vein. Conclusions The intracavitary ECG can be extracted with the microguidewire of PICC system. The amplitude of P wave is related to the location of PICC tip, which can be used to guide the PICC insertion and positioning.

Asthma ; prevention & control ; therapy ; Child ; Disease Management ; Family ; Humans

Objective To discuss the relationships between regular pattern changes of plasma fibrinogen (Fib),D-dimer and fibrinogen degradation products (FDP) levels and the recent dissolution of thrombus in patients with pulmonary embolism (PE) in 14 days after treatment.Methods A prospective study was conducted.PE patients admitted to Departments of Respiratory Disease in 4 hospitals from January 2015 to March 2016 were enrolled and all of them were treated with thrombolysis and/or anticoagulation after admission.The computed tomographic pulmonary angiography (CTPA) was examined pre-treatment and 14 days post-treatment in PE patients.The pulmonary artery obstruction index (PAOI) was assessed according to the Mastora scoring method to estimate the thrombus load.The plasma Fib,D-dimer and FDP levels were measured before and on 1,2,3,5,7 and 14 days after treatment,and the relationships between the change regularities of these parameters and PAOI were also analyzed.Results A total of 42 PE patients were enrolled.The curve change of coagulation-fibrinolytic system parameters in 14 days after treatment showed that the Fib level was raised to its peak on the 3rd day after treatment and then decreased (g/L:4.24 ± 1.45 vs.3.83 ± 1.56),representing that its curve change was in accordance with the quadratic model (P =0.095).After treatment,the D-dimer and FDP levels were kept declining,they were reached the valley on 14th day [D-dimer (mg/L):1.58 ± 1.38vs.8.84 ± 6.35,FDP (mg/L):4.23 ± 3.63 vs.23.41 ± 16.54],and their curve changes were in accordance with the cubic model (F was 32.190 and 34.326,respectively both P =0.000).The PAOI variation before and 14 days after treatment [(18.77 ± 14.22)％] was not correlated with Fib variation [(1.20 ± 0.93) g/L,r =-0.194,P =0.219],but was positively correlated with D-dimer variation [(7.29 ± 7.10) mg/L] and FDP variation [(19.29 ± 18.67) mg/L,r was 0.556 and 0.460,respectively;P was 0.020 and 0.002,respectively].Conclusions The D-dimer and FDP levels are kept falling in PE patients after treatment,suggesting that the pulmonary artery embolus is being dissolved.