2.Effect of MDG-1, a polysaccharide from Ophiopogon japonicas, on diversity of lactobacillus in diet-induced obese mice.
Lin-lin SHI ; Yuan WANG ; Yi FENG
China Journal of Chinese Materia Medica 2015;40(4):716-721
For understanding the effect of MDG-1, a water-soluble β-D-fructan polysaccharide from Ophiopogon japonicas, on intestinal microecological balance, especially on the changes of lactobacillus, sixty 8-week-old male C57BL/6J mice were given a high-fat diet for six weeks and were also gavaged with saline once a day simultaneously. Then the mice which is below 30 grams or dropped more than 10% through lavage were eliminated and the rest were randomly divided into four groups: diet-induced obese (DIO) model group (n = 12, gavaged with saline), low-dose MDG-1 group (n = 12, gavaged with MDG-1, 75 mg · kg(-1)) , medial-dose MDG- 1 group (n = 12, gavaged with 150 mg · kg(-1)), and high-dose MDG-1 group (n = 12, gavaged with 300 mg · kg(-1)) according to the weight and blood glucose; the model group and MDG-1 group were placed on a high-fat diet while the normal control group (n = 12, gavaged with saline) were kept on a low-fat diet through the experiment. After 12-weeks of treatment, feces samples were collected and cultured for intestinal microecological balance analysis. Then the intestinal probiotics were cultured through traditional methods combined with modified gradient gel electrophoresis (DGGE) method. The changes of lactobacillus in each treatment group were also detected by a statistical analysis of the total number of the intestinal flora. We have established the phylogenetic tree by 16S rDNA sequencing and use some molecular identification methods such as PCR-DGGE to analyse the changes of the dominant bacteria floras, and also get the pure culture. In conclusion, different concentrations of MDG-1 can increase the number of the intestinal probiotics, especially Taiwan lactobacillus and Lactobacillus murinus, and improve their diversity and promote proliferation in a dose-dependent way.
Animals
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Biodiversity
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Diet, High-Fat
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adverse effects
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Dietary Carbohydrates
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administration & dosage
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analysis
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Humans
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Intestines
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drug effects
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metabolism
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microbiology
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Lactobacillus
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classification
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drug effects
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genetics
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growth & development
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Obese
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Molecular Structure
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Obesity
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drug therapy
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metabolism
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microbiology
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Ophiopogon
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chemistry
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Phylogeny
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Plant Extracts
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administration & dosage
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chemistry
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Polysaccharides
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administration & dosage
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chemistry
3.Effects of PPAR-gamma agonist and MMP-2 on formation of atherosclerosis plaque in rabbits.
Feng LUO ; Zhao-hui WANG ; Lin-lin DU ; Jue WANG
Chinese Journal of Pathology 2007;36(8):556-557
Animals
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Aorta
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metabolism
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pathology
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Atherosclerosis
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etiology
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metabolism
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pathology
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Cholesterol, Dietary
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Male
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Matrix Metalloproteinase 2
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metabolism
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PPAR gamma
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agonists
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Rabbits
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Random Allocation
5.Long-term effect of quad helix expansion of dental arch*☆
Feng WANG ; Huaixiu LU ; Songshan LIN
Chinese Journal of Tissue Engineering Research 2013;(28):5177-5183
BACKGROUND: Rapid expansion of the dental arch is an effective way to rapidly expanse the jaw. Compared with rapid expansion, the slow expansion has higher stability and less recurrence, but the reports on the long-term stability of quad helix expansion are rare. OBJECTIVE: To retrospectively analyze the clinical effect of quad helix expansion in orthodontics. METHODS: Twenty-two subjects with dental arch stenosis in mixed dentition and early permanent dentition who experienced an expansion of at least 3 mm with quad helix appliance were selected for this study. Plaster dental casts and posteroanterior radiographs were evaluated at the beginning of the treatment (T1), at the completion of phase I quad helix expansion or ful treatment (T2), and approximately 2 years fol owing the completion of treatment (T3). The distance between two first molars was measured on the model. J point was drawn on the posteroanterior radiograph in order to measure the distance between the bilateral base bones and the molar inclination, as wel as to evaluate the corrective and orthopedic effects of dental arch expansion. RESULTS AND CONCLUSION: Compared with that before expansion, the first permanent molar inclination and the distance between base bones on two sides were significant increased after quad helix expansion; there were no significant differences in the distance between two first permanent molars, first permanent molar inclination and the distance between bilateral base bones on two sides when compared after quad helix expansion and after 2-year fol ow-up (P > 0.05). The results indicate that the long-term effect of quad helix expansion is stable with orthopedic effect.
6.Effects of strain force on the expression of osteoclast differentiation factor and osteoclasto-genesis inhibitory factor in human periodontal ligament cells
Feng WANG ; Zhu LIN ; Yongming LI
Journal of Practical Stomatology 2001;0(01):-
Objective:To investigate the effects of strain force on t he expression of osteoclast differentiation factor(ODF) and osteoclasto-genesis i nhibitory factor(OCIF) in human periodontal ligament cells (HPDLCs). Met hods: HPDLCs were subjected to cyclic strain force for 0, 6, 12 and 24 h ours, mRNA expression of ODF and OCIF were determined by RT-PCR. Result s:After treatment of the cells for 0,6,12 and 24 hours the ODF/?-actio n values were 0.7280?0.0261,0.6831?0.0411,0.5801?0.2230 and 0.4572?0.0373( P0.05) respectively.Conclusion:Strain force may decrease the expression of ODF and increase the expression of OCIF.
7.Influence of Hyperthyroidism and Hypothyroidism on Serum Levels of Three Kinds of Cytokines
Feng WEI ; Suhua WANG ; Lin BAI
Journal of Chinese Physician 2001;0(09):-
0 05). The serum levels of both IL-8 and TNF (P
9.Study on Artemisinin Transdermal Delivery Systems
Naijie WANG ; Lin QIU ; Feng DU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(08):-
Azone. PG combined with azone showed a higher synergic effect of enhancing effectiveness. Conclusion National Starch polyacrylate pressure-sensitive adhesive was the most effective pressure-sensitive adhesive matrix, PVP-K30 was the most effective crystallization inhibitor, PG combined with azone were the most effective penetration enhancers.
10.Effect of Permeation Enhancers on the Percutaneous Absorption of Artesunate in Vitro
Lin QIU ; Naijie WANG ; Feng DU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(10):-
IPA.Skin permeation was significantly enhanced by increasing the loading concentration of OA or NMP,larger IPA amount(8%) or PG amount(20%) could inhibit the permeation of artesunate.Conclusion Oleic acid was found to be the most efficient enhancer for artesunate,10% is the most effective concentration.