Objective To explore the expression and clinical significance of hypoxia-inducible factor 1α(HIF-1α)and glucose transporter-1(GLUT-1)in human lung adenocarcinoma cell line A549 and in elderly primary lung cancer tissue under hypoxia. Methods MTT assay was used to test the effect of CoCl2-induced hypoxia on the growth activity of lung adenocarcinoma cell line A549.Reverse transcription-polymerase chain reaction analysis(RT-PCR)and Western blot were used to determine the changes of HIF-1α and GLUT-1 in lung adenocarcinoma cell line A549 under hypoxia.The expressions of HIF-1α and GLUT-1 were detected by using immunohistochemical method and RT-PCR in 60 specimens of lung carcinoma and 26 benign lung tissues in elderly patients with pulmonary diseases.Their interrelations and their relationship with clinicopathologicaI characteristics of the tumors were analyzed. Results The growth activity of lung adenocarcinoma cell line A549 was increased under hypoxia.RT-PCR analysis indicated that the mRNA expressions of HIF-1α and GLUT-1 were 0.69±0.08,0.65±0.09 after hypoxia exposure for 24 hours,and 0.99±0.16,0.83±0.11 after hypoxia exposure for 48 hours,which were higher than those in control group (0.47±0.11,0.40±0.08,P＜0.01).The results from Western blot showed that the protein expressions of HIF-1α and GLUT-1 were 1.57±0.29,1.66±0.28 after hypoxia exposure for 24 hours,and 2.31±0.46,2.22±0.34 after hypoxia exposure for 48 hours,which were higher than those in control group(0.93±0.07,1.06±0.17,P＜0.01).In specimens of lung carcinoma,the positive expression rates of mRNA and proteins of HIF-1α and GLUT-1 were higher than those in benign lung tissues(all P＜0.01).Compared with the mRNA and protein levels of HIF-1a and GLUT-1 in lung carcinoma tissues of Ⅰ and Ⅱ stage,there were marked increases in Ⅲ and Ⅳ stage (all P＜0.05).The expressions of HIF-1α and GLUT-1 in lymph node metastasis group were higher than those in group without lymph node metastasis(all P＜0.05).There were positive correlations between the mRNA and protein expressions of HIF-1α and between the mRNA and protein expressions of GLUT-1(r1=0.527,r2=0.408,respectively,both P＜0.01). Conclusions CoCl2 can up-regulate the expressions of HIF-1α and GLUT-1 in lung adenocarcinoma cell line A549 by inducing hypoxia.The hypoxia-mediated effects on them have the potential to make lung cancer cells further grow and promote hypoxie tolerance.The overexpressions of HIF-1α and GLUT-1 are closely correlated with the progression of lung carcinoma.Combined detection of HIF-1α and GLUT-1 may be helpful for the diagnosis and prognosis prediction of primary lung cancer in elderly patients.

Adenocarcinoma, Mucinous ; pathology ; Adenoma ; pathology ; Adolescent ; Breast Neoplasms, Male ; metabolism ; pathology ; secretion ; surgery ; Carcinoma ; metabolism ; pathology ; secretion ; surgery ; Diagnosis, Differential ; Humans ; Keratin-18 ; metabolism ; Keratin-8 ; metabolism ; Male ; Mastectomy, Modified Radical ; Proto-Oncogene Proteins c-kit ; metabolism ; S100 Proteins ; metabolism

ObjectiveDiabetes is an independent risk factor for coronary artery bypasss grafting(CABG). Off pump coronary artery bypasss (OPCAB) experience in 251 cases was reviewed to determine whether diabetes wou ld be applicable in OPCAB procedures.MethodsConsecutive 251 patients underwent OPCAB over 12 month period. This study included 71 diebetic patients (DM group) and 180 nondiabetic patients (NDM group). Preoperative v ariables were compared between the two groups by univariate analysis.R esultsNo differences were found regarding the length of stay in cardio intensive care unit [DM group(2.4?0.3)d; NDM group (2.4?0.3) d;P=0. 386], and sternal complication (DM group: 5.7%;NDM group: 3.9%;P=0.511) . In hospital complications were as follows: death rate(DM group: 2.8%; NDM gr oup: 1.1%; P=0.680); stroke (DM group: 2 8%; NDM group: 1 7%; P=0 623 ); hemofiltratioin renal failure (DM group: 2.8%; NDM group: 0.5%; P=0.194); myocardial infarction(DM group: 0%; NDM group: 0.5%;P=1.000); blood using were more frequent in DM group comparied with NDM group (P=0.111). ConclusionOPCAB in diabetic patients is as safe as in non diabetic patients.

Objective To observe the effect of robot-assisted upper limb training on the recovery of upper limb motor function after stroke.Methods One hundred patients within one month of suffering a stroke were randomly divided into a control group (50 cases) and a therapy group (50 cases).Both groups were given conventional drug therapy and rehabilitation training,while the therapy group also received robot-assisted training once a day,5 days per week for 12 weeks.The Fugl-Meyer assessment for the upper extremities (FMA-UE),the modified Ashworth scale (MAS) and the modified Barthel index (MBI) were used to evaluate the subjects before the experiment and after 4,8 and 12 weeks of treatment.Results During the treatment,the average FMA-UE scores and the average MBI of both groups increased gradually.The treatment group's averages were significantly better than those of the control group at each time point,except that there was no significant difference in elbow and wrist MAS scores.Conclusion Upper limb robots can be used with acute stroke patients in a clinical setting,and they may be beneficial for improving upper limb function and ability in the activities of daily living.

Adult ; Ethylene Dichlorides ; poisoning ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Neurotoxicity Syndromes ; etiology ; pathology ; Young Adult

The purpose was to compare the difference between transgene expressions driven by homologous duplicated carbonic anhydrase (DCA) promoter and foreign CaMV35S promoter in the unicellular green alga, Dunaliella salina(D.salina).The CaMV35S promoter-bar construct and DCA promoter-bar construct into D.salina by a Backon 2000 electroporation system were introduced. After the repeated selections with the phosphinothricin (PPT) of 3mg/L, 3 PPT-resistant phenotype transformants were isolated from the CaMV-bar and DCA-bar pools of transformants of D. salina, respectively. The results of PCR and sequencing showed that bar genes were stably integrated into the genome of D.salina, and Southern bolts showed the number of transgene copy had no significant difference between both promoters. Semi-quantitive RT-PCR indicated that the mRNA levels of bar gene were higher in DCA-bar transformants than the CaMV-bar transformants, and could be increased under the induction of high salt in DCA-bar transformants but not in the CaMV-bar transformants. Analysis of growth rate of transformants showed DCA-bar transformants achieved the log stage faster than the CaMV-bar transformants. It is concluded that the homologous promoters have more advantages than the foreign promoters in the transgenic D.salina.

Microbial metabolomics is a subject that chiefly studying all the low molecular weight metabolites in an organism or cells during their growing process. The progress of analytical technology promotes microbial metabolomics to make advancement. In this paper, the commonly used analytical technology, sample preparation and its application were discussed and the prospects of the analytical methods were also discussed.

The purpose of this study is to explore the feasibility of wheat germ agglutinin (WGA) modified liposome as a vehicle for ophthalmic administration. Liposome loaded with 5-carboxyfluorescein (FAM) was prepared by lipid film hydration method. WGA was thiolated and then conjugated to the surface of the liposome via polyethylene glycol linker to constitute the WGA-modified and FAM-loaded liposome (WGA-LS/FAM). The amount of thiol groups on each WGA molecule was determined, and the bioactivity of WGA was estimated after it was modified to the surface of liposome. The physical and chemical features of the WGA-modified liposome were characterized and the ocular bioadhesive performance was evaluated in rats. The result showed that each thiolated WGA molecule was conjugated with 1.32 thiol groups. WGA-LS/FAM had a mean size of (97.40 +/- 1.39) nm, with a polydispersity index of 0.23 +/- 0.01. The entrapment efficacy of FAM was about (2.95 +/- 0.21)%, and only 4% of FAM leaked out of the liposome in 24 h. Erythrocyte agglutination test indicated that after modification WGA preserved the binding activity to glycoprotein. The in vivo ocular elimination of WGA-LS/FAM fitted first-order kinetics, and the elimination rate was significantly slower than that of the unmodified liposome, demonstrating WGA-modified liposome is bioadhesive and suitable for ophthalmic administration.
Absorption, Physicochemical ; Adhesiveness ; Administration, Ophthalmic ; Animals ; Drug Carriers ; Eye ; metabolism ; Fluoresceins ; chemistry ; Liposomes ; administration & dosage ; chemistry ; pharmacokinetics ; Male ; Particle Size ; Polyethylene Glycols ; chemistry ; Rats ; Rats, Sprague-Dawley ; Wheat Germ Agglutinins ; administration & dosage ; chemistry ; pharmacokinetics

HCV RNA positive serum was first selected by RT-PCR test kit from several anti-HCV positive sera obtained from Xi'an.HCV RNA extrac ted from the elected sera was converted to cDNA by reverse transcription with ra ndom primer.Half-nested PCR was performed.The amplified product was 852 bp.The purified PCR product was digested by restriction endonucleases and then ligated to epressio vector pET-22b\++.Its nucleotide sequence was determined by dideoxy chain termination method.A comparison of the sequence with several isolates rep orted previously showed that the sequence belonged to HCV type Ⅱ.

ObjectiveTo identify the framework for assessing health related quality of life(HRQOL) of Kaschin-Beck disease(KBD),in order to reflect the impact of KBD on quality of life in patients with the disease.MethodsQualitative descriptive research was adopted.Semi-open ended questions were developed by using the World Health Organization(WHO) definitions of health and quality of life.Group interview and face to face interviews were conducted on 48 patients with KBD and 29 health care experts on KBD in Linyou and Yongshou counties,Shaanxi province,which were higher prevalence areas of KBD.Content template analysis was conducted and the template was based on the WHOQOL-100's framework.ResultsThe framework of HRQOL for KBD included four domains:physical activity,familial/social support,economic and psychological state.There were also eleven facets which were:pain and discomfort,physical function and activity limitation,diet and sleeping,social relationship,concerns of family responsibilities,social support,economic,housing and the surrounding environment,appearance concerns,mental health,and general state of health.The total entries were 69.ConclusionsThe framework for assessing HRQOL of KBD is established.The framework highlights the impact of KBD on the patients' quality of life with higher specificity.