Objective:To probe the correlation of intra-operative hyaluronic acid(HA) changes with hepatic allografts cold ischemia reperfusion injury in liver transplantation.Methods:The patients with orthotopic liver transplantation were assigned to 3 groups:① group A :liver allografts were stored in University of Wisconsin's solution(0℃) for 5-6 h before implantation;② group B :liver allografts were stored in U W's solution(0℃) for 8-9 h before implantation;③ group C :liver allografts were stored in U W's solution(0℃) for 12 h before implantation.The serum samples were taken up at 5 min,60 min,120 min,180 min after the donor liver was transplanted and reperfused to detect the concentration of HA.Results:After donor liver was transplanted and reperfused,serum HA concentration decreased immediately and gradually.HA decreased rate :group A 4.58 ?g/L?min-1,group B 4.16 ?g/L?min-1,group C 2.26 ?g/L?min-1.Serum HA decrease rate was faster in group A and B than that in group C(P

This paper introduces the wavelet transform with multiresolution for liver cancer CT image enhancement. As the wavelet transform with compact support, orthogonality and approximately symmetry, SymletsA (sym4) wavelet family is applied to the two-layer multiscale decompose of the CT image. The decomposed wavelet coefficients are processed with the threshold values of 550, 350 and 250, with the low -frequency region intensified and the high-frequency one weakened. Then the image is reconstructed and thus the image is enhanced. Wavelet transform proves efficient in CT image enhancement.

Modern fully automatic biochemical analyzer makes enzyme measurement accurate, specific and practical, but alarm failure happens sometimes and thus determinate error appears. To find out the inducement to alarm failure, Olympus AU2700 and ADVIA 1650 fully automatic biochemical analyzer are used to measure the serum liver enzyme from 450 cases of post-liver-transplantation patients.

DNA, Fungal ; isolation & purification ; Humans ; Mass Screening ; Occupational Diseases ; diagnosis ; microbiology ; Polymerase Chain Reaction ; Skin ; microbiology ; Tinea ; diagnosis ; microbiology

Objectives To study the influential factors of the von Clauss method and to promote the method for fibrinogen assay in clinical laboratories in China. MethodsKits of foreign and domestic products were used. Calibration curves for fibrinogen determination were established, and calibration plasma was used for comparison. Effects of pH value, thrombin activity, and temperature on the measurement were studied.Results The reproducibility of the von Clauss method using domestic thrombin and related reagents was excellent, the within day CV being 0.039 1, and the between day CV 0.049 7. The percentage variations of calibration plasma determination, with the foreign fibrinogen assay kits using domestic reagents, were +3.17% , +4.76%, and -6.03%, respectively. Determination of a lyophilized plasma using the two sources reagents showed similar results.Conclusions Thrombin and relevant reagents produced in China could be used as a substitute for kits purchased abroad, which would promote the use of von Clauss method in clinical laboratories of China.

Twenty-seven patients with subclinical hyperthyroidism(SH)complicated by paroxysmal atrial fibrillation(PAF)were treated with methimazole plus bisoprolol.All patients were examined by Doppler echocardiogram and 24 h ambulatory electrocardiograms before and 3 months after treatment.Serum FT3,FT4,and TSH levels were measured with RIA.The results showed that low-dose methimazole therapy could improve the left ventricular diastolic function(P < 0.01)and help maintain sinus rhythm.The incidence of subclinical hypothyroidism was low.Low-dose methimazole was effective and safe in patients with SH complicated by PAF.

OBJECTIVE:To improve the ability of vaccine post-marketing surveillance,and clearly define the development di-rection of it in China. METHODS:Though introducing the background,strategic objective and operation objective of Global Vac-cine Safety Blueprint,the situation of vaccine post-marketing surveillance in China was analyzed. RESULTS&CONCLUSIONS:Vaccine post-marketing surveillance have developed fast in China recent years and get generally confirmation. However,compared with the goals in the blueprint,there are some activities should be strengthened,such as active surveillance,signal analysis and da-ta standardization,etc. We should take use of the opportunity offered by WHO and other international organizations to shrink the gap between our country and the advanced countries. Drawing support from WHO and other international institutions,vaccine post-marketing surveillance can step on the new level in China.

ADAM Proteins ; genetics ; ADAM17 Protein ; Animals ; Gene Library ; Genetic Vectors ; Male ; Mice ; Two-Hybrid System Techniques

Objective To analyze the molecular epidemiological characteristics of norovirus (NoV) outbreaks in Qingdao between 2014 and 2016.Methods Stool samples were collected from NoV outbreaks between January 2014 and December 2016 and detected by real-time RT-PCR.NoV open reading frame 1 (ORF1) and ORF2 were partially amplified by RT-PCR.The amplified products were further analyzed by gene sequencing and genotyping.Phylogenetic analysis was conducted by using MEGA 6.0 software package.Results A total of 23 NoV outbreaks, involving 260 cases, were reported during 2014 to 2016.Of all collected stool samples, 128 were positive for NoV including 6 of genogroupⅠ (GⅠ) and 122 of genogroupⅡ (GⅡ).All positive samples were genotyped into 6 genotypes, which were GⅡ.P17-GⅡ.17, GⅡ.P12-GⅡ.3, GⅡ.P7-GⅡ.6、GⅡ.P2-GⅡ.2, GⅠ.Pb-GⅠ.6 and GⅡ.Pg-GⅡ.12.The 23 outbreaks included both single infections and mixed genotype infections, which were 11 of GⅡ.17 single infection, 4 of GⅡ.3 single infection, 3 of GⅡ.17 and GⅡ.3 mixed infection, 2 of GⅡ.17 and GⅡ.6 mixed infection, 1 of GⅠ.6 single infection, 1 of GⅡ.17 and GⅡ.2 mixed infection and 1 of GⅡ.17 and GⅡ.12 mixed infection.Conclusion NoV was an important pathogen responsible for viral diarrhea outbreaks in Qingdao.Several different genotypes were detected.The newly variant GⅡ.P17-GⅡ.17 was the predominant epidemic strain causing norovirus outbreaks in Qingdao during 2014 to 2016.

Aim To investigate the expression of mGluR1 in hippocampus of spontaneously epileptic rats(SER) by control study.Methods Total RNA was extracted from hippocampus of SER and Wistar rats,and mGluR1 mRNA expression was detected by RT-PCR;the expression of mGluR1 protein was detected by immunohistochemistry and Western blot.Results mGluR1 mRNA of SER was lower than that of Wistar rats in hippocampus(P0.05) were found.In positive cells,mGluR1 proteins were enhanced.By Western blot,total mGluR1 protein was down-regulated in SER hippocampus(P