1.Relationship between GRIM-19 and cancers
International Journal of Surgery 2010;37(11):759-762
Gene associated with retinoid-IFN-induced mortality-19(GRIM-19)is originally isolated as a growth suppressive gene using a genetic screen.GRIM-19, initially found in nucleus and mitochondria, is essential for the assembly and functioning of mitochondrial complex I.GRIM-19 involves the regulation of cell proliferation and apoptosis, and low expression or mutation of GRIM-19 contributes to abnormal proliferation.During viral oncogenesis, GRIM-19 may be a general target protein similar to other cellular tumor suppressors.GRIM-19 plays an important role in tumor formation and apoptosis inhibition, and may be as a new tumor marker to early cancer screening.
2.H3 K27 me3 demethylase Jmjd3 regulates proliferation and differentiation of embryonal lung epithelia of mice
Huan FENG ; Chao ZHANG ; Zhekai ZHANG ; Ming ZHANG ; Feng ZHANG
Basic & Clinical Medicine 2015;(9):1176-1181
Objective To investigate the role of H3K27me3 demethylase Jmjd3(KDM6B) during the development of lung in embryonal mice .Methods Jmjd3 knockout embryos of E 19.5 mice were examined by HE , PAS and immnohistochemistry assays .Results The developmental defects of the lung of Jmjd 3 heterozygous ( Jmjd3 +/-) embryos were mild is compared to Jmjd3 +/+embryos.However, Jmjd3 -/-mice suffered from the severe hypoplasia of lung tissue .Differentiated defects of ciliated cell , Clara cell , type Ⅰ and Ⅱ alveolar epithelial cells were ob-served in Jmjd3 -/-embryos.The index of cell proliferation was increased in Jmjd3 -/-embryos as compared to wildtype and Jmjd3 +/- embryos.No difference in apoptosis profile was found in these embryos .Conclusions Jmjd3 is essential for proliferation and differentiation of embryonal lung epithelia of mice .
3.Content Detemination of Paeoniflorin in Refined Coronary Tablets by HPLC
Chao MENG ; Shaohua FENG ; Jing ZHANG
China Pharmacy 2005;0(22):-
OBJECTIVE:To establish a HPLC method for content determination of paeoniflorin in refined coronary tablets.METHODS:HPLC was performed on Kromasil C18 column at room temperature,the mobile phase consisted of acetonitrile-0.2%phosphoric acid solution(13∶87),the detection wavelength was 230nm with flow rate 1ml/min and sample size 10?l.RESULTS:Paeoniflorin was linear with the peak area in the range of 0.317?g~1.587?g(r=0.9 999),the average recovery was 100.1%(RSD=1.46%).CONCLUSION:The established method is simple,reliable,reproducible and can be used for the quality control of refined coronary tablets.
4.Surgical treatment for tumor involved inferior vena cava at the upper segment of kidney
Xiang FENG ; Chao SONG ; Lei ZHANG
Chinese Journal of Digestive Surgery 2015;14(9):733-736
Objective To investigate surgical treatment for tumor involved inferior vena cava at the upper segment of kidney.Methods The clinical data of 35 patients with tumor involved inferior vena cava at the upper segment of kidney who were admitted to Changhai Hospital affiliated to the Second Military Medical University from January 2007 to May 2015 were retrospectively analyzed.All the patients received preoperative imaging examinations to insure the site and range of inferior vena cava involvement at the upper segment of kidney.Renal cell carcinomas with inferior vena cava involvement were found in 19 cases,leiomyosarcomas of inferior vena cava in 5 cases,leiomyomatosis involving inferior vena cava in 3 cases,adrenocortical carcinoma involving inferior vena cava in 3 cases,liver cancer involving inferior vena cava in 2 cases,right adrenal pheochromocytomas in 2 cases,retroperitoneal fibrosarcoma involving inferior vena cava in 1 case.According to tumor involvement types,the different surgical approaches,planes and method of inferior vena cava exclusion,reconstruction method and prevention of tumor embolus detachment were selected.Patients were followed up by outpatient examination and telephone interview till May 2015.Results Among 19 patients with renal cell carcinomas with inferior vena cava involvement,10 patients were placed inferior vena cava filters through internal jugular vein before surgery,10 patients underwent total hepatic vascular exclusion and 9 patients underwent intrahepatic inferior vena cava exclusion.All the 19 patients received tumor resection and inferior vena cava embolectomy.Of the 5 patients with leiomyosar-comas of inferior vena cava,3 patients underwent total hepatic vascular exclusion and 2 patients underwent intrahepatic inferior vena cava exclusion.The diseased segments of 5 patients were resected,including 4 patients of artificial vascular graft and 1 patient complicated with resection of right kidney receiving simple ligation of inferior vena cava and left renal vein at proximal and distal tumors.Of the 3 patients with leiomyomatosis involving inferior vena cava,2 patients received total hepatic vascular exclusion and 1 was treated surgically under cardiopulmonary bypass.All the 3 patients underwent inferior vena cava embolectomy and hysterectomy.Three patients with adrenocortical carcinoma involving inferior vena cava and 2 patients with liver cancer involving inferior vena cava underwent total hepatic vascular exclusion.Among the 5 patients,4 had direct suture after tumor removal combined with partial inferior vena cava resection,and 1 had patch repair after partial inferior vena cava resection.Two patients with right adrenal pheochromocytomas were exposed proximal and distal lifting devices of inferior vena cava without clamp,and the tumors were peeled off completely.Intraoperative death happened in the patient with retroperitoneal fibrosarcoma involving inferior vena cava who was prepared to undergo intrahepatic inferior vena cava exclusion but encountered intraoperative pulmonary embolism due to tumor thrombus shedding.Thirty-four patients of 35 patients underwent operation successfully without serious perioperative complications and a patient died in the perioperative period.The mean operation time,volume of intraoperative blood loss and duration of postoperative hospital stay were 2.8 hours (range,1.5-5.0 hours),2 000 mL (range,400-5 000 mL) and 9.2 days (range,6.0-16.0 days).Thirty-four patients were followed up for a median time of 12 months (range,1-60 months).During the follow-up period,a patient with leiomyosarcomas of inferior vena cava and 2 patients with adrenocortical carcinoma involving inferior vena cava died of tumor recurrence,a patient with liver cancer had tumor recurrence,other patients were tumor-free survival.Conclusions Inferior vena cava at the upper segment of kidney is not contraindication for tumor resection.The appropriate way to expose,clamp and reconstruct are selected to safely remove the tumor based on extension and method of tumor involving inferior vena cava.
5.Role of Numb in tumors
Chao WANG ; Chuyao ZHANG ; Weiwei FENG
Journal of International Oncology 2012;39(7):541-544
Numb,one of the important cell fate determinants,plays a key role in regulation of nerve system development,and it is closely related to the occurrence of tumors.It is proven that Numb is implicated in the processes of occurrence,invasion and metastasis of tumors through regulating the way of cell division,cell polarity,epithelial-mesenchymal transitions and several signal pathways,e.g.,Notch,Hh,p53.The expression levels of Numb in cells are related to the malignant degree and prognosis of tumors.It is vital to explore the role of Numb in tumors for treating tumors at the standpoint of Numb.
6.Three-dimensional finite element analysis of maxillary anterior teeth retraction force system in light wire technique.
Xiangfeng ZHANG ; Chao WANG ; Xi XIA ; Feng DENG ; Yi ZHANG
West China Journal of Stomatology 2015;33(3):291-295
OBJECTIVEThis study aims to construct a three-dimensional finite element model of a maxillary anterior teeth retraction force system in light wire technique and to investigate the difference of hydrostatic pressure and initial displacement of upper anterior teeth under different torque values of tip back bend.
METHODSA geometric three-dimensional model of the maxillary bone, including all the upper teeth, was achieved via CT scan. To construct the force model system, lingual brackets and wire were constructed by using the Solidworks. Brackets software, and wire were assembled to the teeth. ANASYS was used to calculate the hydrostatic pressure and the initial displacement of maxillary anterior teeth under different tip-back bend moments of 15, 30, 45, 60, and 75 Nmm when the class II elastic force was 0.556 N.
RESULTSHydrostatic pressure was concentrated in the root apices and cervical margin of upper anterior teeth. Distal tipping and relative intrusive displacement were observed. The hydrostatic pressure and initial displacement of upper canine were greater than in the central and lateral incisors. This hydrostatic pressure and initial intrusive displacement increased with an increase in tip-back bend moment.
CONCLUSIONLingual retraction force system of maxillary anterior teeth in light wire technique can be applied safely and controllably. The type and quantity of teeth movement can be controlled by the alteration of tip-back bend moment.
Finite Element Analysis ; Humans ; Incisor ; Maxilla ; Tooth Movement Techniques ; methods ; Tooth Root ; Torque
7.Expressions of Cyclooxygenase-2 and Hypoxia-Inducible Factor-1? in Hepatocellular Carcinoma
Chao JIANG ; Feng ZHANG ; Shaojun WANG ; Qifei ZOU
Chinese Journal of Bases and Clinics in General Surgery 2003;0(02):-
Objective To investigate the relationship of expressions of cylooxygenase-2(COX-2) and hypoxia-inducible factor-1?(HIF-1?) in hepatocelluar carcinoma(HCC) and the possible antineoplastic mechanism of selective COX-2 inhibitor.Methods The expressions of COX-2 and HIF-1? in 53 cases of HCC tissues were detected immunohistochemically.Western blot was employed to evaluate the effects of variant concentration of COX-2 inhibitor meloxicam on expression of HIF-1? in Cobaltchloride-stimulated SMMC-7721 cell.Results Of 53 tumor tissues,the expression of COX-2 was 22/53(41.5%) strongly positive stained,11/53(20.8%) positive stained,and 20/53(37.7%) negative stained.Meanwhile the expression of HIF-1? was 18/53(34.0%) strongly positive stained,18/53(34.0%)positive stained,17/53((32.1%)) negative stained.The expression of COX-2 was correlated positively with HIF-1? in HCC(r=0.440,P
8.Psychological Characteristic and Crisis Intervention in Patients Preliminarily Diagnosed with Cancer
Chao ZHANG ; Yanying FENG ; Yaofen WU ; Yanhua FAN
Chinese Medical Ethics 1995;0(04):-
Unfavorable psychic reactions or psychological crisis always occur in patients preliminarily diagnosed with cancer.By analyzing the psychological activities and affecting factors,we try to discuss the measures of psychological crisis intervention for patients preliminarily diagnosed with cancer.
9.Effect of vaIproic acid on radiosensitivity to breast cancer ceIIs
Xipeng ZHAO ; Yue LUO ; Chao DONG ; Fengmei ZHANG ; Zhihui FENG
Chinese Journal of Pharmacology and Toxicology 2015;(2):247-252
OBJECTIVE To study the effect of valproic acid ( VPA) on radiosensitivity to MCF7 breast cancer cells. METHODS MCF7 cells were pretreated with VPA 0.5 and 1 mmol.L-1 for 0, 24, 48 and 72 h respectively, irradiated with 8 Gy lR, and at 6 h post-lR, the γ-H2 AX foci formation in MCF7 cells was tested by immunofluorescence assay. MCF7 cells were pretreated with VPA 0.5 and 1 mmol.L-1 for 72 h, irradiated with 4 Gy lR, and at 48 h post-lR, the cell survival rate was detected by MTT assay. MCF7 cells were pretreated with VPA 0.5 mmol.L-1 for 24 h, and then irradiated according to the amount of cells: 2 Gy (500 and 1000 cells per plate), 4 Gy (2000 and 4000 cells per plate), 6 Gy (8000 and 16000 cells per plate), and the cloning efficiency was calculated. MCF7 cells were pretreated with VPA 0.5 and 1 mmol.L-1 for 0, 24, 48 and 72 h respectively and the cell cycle profile was analyzed via flow cytometry. RESULTS After treatment with VPA alone for 24 h, MCF7 cells showed a significant increase in the amount of γ-H2 AX foci formation ( P < 0. 01). lt was also found that VPA increased lR-induced γ-H2 AX foci formation, which obviously prolonged the pretreatment time of VPA(P<0.01) in a time-dependent manner(r=0.98, P<0.05). VPA 0.5 and 1 mmol.L-1 had the same effect on γ-H2 AX foci formation. Furthermore, VPA was able to cause a significant decrease in lR-induced clonogenic survival but an increase in lR-induced cytotoxicity by MTT assay. Also, VPA alone decreased the plating efficiency of MCF7 cells. However, the cycle profile of MCF7 cells treated with both VPA 0.5 and 1 mmol.L-1 was not changed. CONCLUSION Without affecting the cell cycle profile, both the safe and critical dose of VPA used in clinical epilepsy treatment can significantly increase the accumulation of DNA double strand breaks in the cells and sensitize the cells to lR treatment, suggesting that VPA can induce radio-sensitization of breast cancer cells.
10.Effect of Water Extract from Salvia Miltiorrhiza on the Hair Regrowth in Testosterone-induced Alopecia in Mice
Zhibi ZHANG ; Chao DONG ; Jiao MA ; Guohua FENG
Journal of Kunming Medical University 2016;37(11):23-27
Objective To study the role of water exact from salvia miltiorrhiza (SM) in promoting hair regrowth in testosterone (T)-induced alopecia in mice.Methods Pathological alopecia mice were induced by T and the mice were randomly divided into control group,model group,Zhanggvanp 101 group,SM low-dose group,SM middle-dose group,SM high-dose group (20,40,80 mg/ml,respectively).The effects of SM on hair regrowth were evaluated by measuring hair length,weight and the number of hair follicle.The concentration of serum T and estrodiol (E2),the content of skin VEGF,MDA,SOD,and GSH-Px were measured after external inunctum for 21 days.Results (1) SM significantly promoted regrowth hair length,weight and the number of hair follicle (P<0.05) (2) SM significantly balanced the hormones by reducing levels of T and increasing E2 (P<0.05) (3) SM significantly reduced the content of MDA by increasing the activity of SOD and GSH-Px (P<0.05) (4) SM significantly increased the level of VEGF (P<0.05) Conclusion In the pathological alopecia mice induced by T,SM can effectively promote the hair regrowth,with the possible mechanism of balancing hormones,being anti-oxidant and improving VEGF expression.