The ocular fundus changes and the damage of visual function were various at different stages of diabetic retinopathy (DR). To get hold of timing and different therapic method correctly of early diagnosis, whole body treatment, laser photocoagulation and vitreous-retina surgery and adopting targeted interventions could help patients receiving the most reasonable and effective treatment at different stages,both of them are keys to reduce the damage of visual function.

Objective To investigate the effect of different doses of rosuvastatin Calcium in the treatment of coronary heart disease complicated with hyperlipemia and its effect on blood lipid level.Methods 100 patients with coronary heart disease complicated with hyperlipemia were randomly divided into the three groups according to the number table methods,the patients in group A received rosuvastatin Calcium 5mg/d,group B was given 10mg/d, group C was given 20mg/d.A treatment course was 4 weeks,and continuous treatment lasted 3 courses.The effects after treatment and serum TC,TG,LDL-C and HDL-C levels before and after treatment of the three groups were compared,and the occurrence of adverse reactions in the three groups of patients was recorded.Results The effective rate of C group in the treatment of coronary heart disease was 87.9%,the effective rate in the treatment of hyperlipi-demia was 93.9%,which were significantly higher than those in group A and group B (χ2 =6.54,P<0.05 );The serum TC,TG,HDL-C and HDL-C levels of three groups after treatment were significantly improved compared with before treatment,and which in C group were improved better than the other two groups(F=5.45,P<0.05);There was no significant adverse reactions in the three groups during treatment.Conclusion Large dose rosuvastatin Calci-um has a significant therapeutic effect in treatment of coronary heart disease complicated with hyperlipemia,which can effectively regulate blood lipid levels,with no obvious adverse reaction and high security,which is worthy of clinical application.

Intravitreal injection of antiangiogenic agents is widely used to treat retinal vascular disease.This therapy can induce regression of neovascular vessels; reduce intraocular inflammation and retinal vascular permeability,and control macular edema.However the action period of these agents is short,and thus this therapy need repeated injections which cause higher operation risk and cost.Retinal laser photocoagulation therapy can close retinal capillary non-perfusion area and neovascular vessels,reduce macular edema caused by vascular leakage.However,as its therapeutic effect is based on the destruction of the retinal tissues in the lesion area,this therapy need longer time to show its effects.When the disease is controlled by this method,it may already induce some structural irreversible damages to the retina,especially the macular.This is why the visual acuity is not satisfactory in some patients,even though the disease get controlled,macular edema gets disappeared and anatomical structure of retina get improved.Properly evaluating all the pros and cons of retinal photocoagulation and intravitreal injection of antiangiogenic agents,will allow us to explore a better way to combine these two therapies to treat retinal vascular diseases.

OBJECTIVE:To evaluate the importance of monitoring serum concentration of Sodium Valproate(VPA)in the treatment of epileptic children.METHODS:Serum VPA concentration was determined by Chemiluminescence Enzyme Immunoassay(CLEIA),and the correlation between serum drug concentration and the daily dosage(mg?kg-1?d-1),dosage form,individual differences,blood sampling time and the clinical efficacy was analyzed.RESULTS:The serum VPA concentration which was lower than 50 mg?L-1 was found in 35.45% of the cases,and that which was higher than 100 mg?L-1 was found in 8.99%.CONCLUSION:The serum VPA concentration which was not in the therapeutic window can be resulted from multiple factors,and the monitoring results of the serum concentration should be analyzed comprehensively and made good use of.

Objective:To study the relationship between the cell density and the cell proliferation phenotype. Methods: Plate clonality assays was used to measure the impact of cell density to cell clonality and cell cycle in BT325、786-0、293、C6 and NIH3T3 cell lines. Results: The clonality decreased when the cells grown to confluence in NIH3T3 and 7860 cell lines respectively.It seem need more cells to decrease the clonality in 293 cell line but there is no relationship between cell density and cell clonality in BT325 and C6 cell lines.Cell cycle analysis show that cell density have no effect on BT325 and C6 but on 786-0、293 and NIH3T3 cell lines. Conclusion: There might exist preventer or preventers,which is proportional to the number of cells,of immortal stem cell to expand.In addition,the rate of stem cell expansion is proportional to that of cell mitosis in immortal cell lines.

Objective To investigate the correlation between mechanical ventilation (MV) initiation time during cardiopulmonary resuscitation (CPR) and the prognosis of patients with in-hospital cardiac arrest (IHCA) in emergency . Methods Retrospective analysis of clinical records of patients with IHCA in emergency from January 2011 to April 2016 treated in Zhangye People's Hospital of Hexi University was performed. Patients with restoration of spontaneous circulation (ROSC) and were on MV with aged over 18 years were divided into early treated group (≤ 10 minutes) and later treated group (> 10 minutes) according to the initiation time of MV. Corneal reflex, pupillary reflex, pain-avoidance responses and motor response 24 hours after CPR, neurological function and cure rate of the two groups were analyzed. Results 210 patients were selected into our study including 130 males and 80 females (mean age: 60.24±13.17 years). There were no significant differences in gender, age, type of heart rate and etiological factor of cardiac arrest (CA) between the MV early stage group (124 cases) and the MV late stage group (86 cases). The restoration of corneal reflex, pupillary reflex, pain-avoidance responses, motor response and achievement ratio of CPR in early group were higher than those of later group (respectively, 59.68% vs. 31.40%, 59.68% vs. 31.40%, 54.84% vs. 24.42%, 54.84% vs. 24.42%, 70.16% vs. 51.16%, all P < 0.01); The D-dimer levels in the early group patients were significantly lower than those in the later group (μg/L: 478.39±57.21 vs. 510.05±62.83, P < 0.01). However, no statistical difference was observed between the two groups with respect to pH (7.24±0.72 vs.7.13±0.67, P > 0.05); The average hospitalized day of the early group was significantly shorter than that of the later group (days: 24.15±3.04 vs. 30.28±4.17, P < 0.01); Besides, the early group showed a higher survival rate at discharge and had more cases with neurologic level of grade 1-2 than those of the later group (Respectively, 41.94% vs. 26.74%, P < 0.05; 44.35% vs. 15.12%, P < 0.01). Conclusion Initiation MV on IHCA patients in the early stage of CPR (≤ 10 minutes) could help improve the hypoxic condition and prognosis of neurological function, and increase the achievement ratio of CPR.

Objective To evaluate the efficacy of seretide (fluticasone/salmeterol) in treatment of patients with cough variant asthma(CVA). Methods 86 CVA patients were treated with fluticasone/salmeterol propionate 8 weeks, after 8 weeks, 86 patients were randomly assigned to treatment group and control group, each group with 43 patients, the treatment group continued medicine for 4 weeks, the control group without any treatment.The two groups of patients FENO measurement values, cough symptom score before treatment and after treatment for 8 weeks, 12 weeks were compared. Results After treatment for 8 weeks, cough symptom scores and FeNO in the two groups values decreased significantly compared with those before treatment(P<0.05), 12 weeks after treatment, treatment group cough product, FeNO values, were lower than in control group(P<0.05).Conclusion Fluticasone salmeterol powder inhalation can effectively improve the symptoms of cough cough variant asthma.Symptom score and FENO value can be used as a monitoring index to evaluate the therapeutic effect of CVA, and the FENO value is more effective, which has the advantages of non-invasive, simple, safe, rapid and repeatable.

Objective]To summarize professor and national physician master Guo Chengjie asarum clinical application experience, to do good to the practitioners. [Methods] Through analyzing Guo Lao clinical experience of the medicinal nature of the composition and toxicity of understanding, to adapt to the conditions and the usage and dosage of the original understanding, analyse and summarize the experience of clinical application of asarum. [Results] Professor Guo Chengjie applies asarum poliomyelitis to cold organs and blockage for the treatment of diseases such as lung;Drug compatibility exalts development, and expands the application in the mammary gland disease by asarum;On the dosage of asarum characteristics and decoction methods give unique insights;Guo Lao especially emphasizes the importance of dialectical medication. [Conclusion] Pro. Guo's application experience and innovation, asarum clinical curative effect is good, is worth using for reference.

Retinal hemorrhage can be divided into intravitreal, subhyaloid, subinner limiting membrane, superficial retinal, deep retinal, subretinal and subpigment epithelial hemorrhage. Each of these hemorrhages has a characteristic and recognizable appearance. Comprehending the classification and characteristic of retinal hemorrhage has important value in diagnosis, differential diagnosis and guideline for treatment.(Ophthalmol CHN, 2009, 18: 221-224)

Objective　To investigate the validity of the Chinese version of the Symptom Check-List-90 (the SCL-90) in Chinese population. Methods　A total of 624 normal person and 268 out patients were examined with the SCL-90. Results　（1）The internal consistency of the original subscales was found to be good and Cronbach's coefficient alpha ranged from 0.78 to 0.90; （2）Discrimination function analysis based on the 9 original subscales showed that the power of the SCL-90 in discriminating the patients and the community was good with a corrective rate of 80.6%; （3）Factor analysis on the items of the questionnaire yielded a very strong un-rotated first factor which could represent other factors; （4）High inter-correlations were found between the 9 original subscales of the SCL-90 with a correlation coefficient ranging from 0.39 to 0.79. Conclusion　(1) The SCL-90 is acceptable as a research setting and psychiatric screening inventory; (2) The SCL-90 does not have potential as an inventory to distinguish different diagnostic groups; (3) The SCL-90 is not a multidimensional inventory.