Objective To observe the student's endocrine metabolism in senior high school during different periods.Methods 328 senior middle school students in different periods were investigated and the blood concentration of adrenaline(N),norepinephrine(NE),cortisol(CS),interleukin-2(IL-2),interleukin-6(IL-6)were measured by radioimmunoassay and Magnetic Solid Phase Separation-Enzyme Immunoassay,and at the same time,students in vocational high school were contrasted.Results As the university entrance examination was coming,the values of N,NE and CS were apparently rising,while the values of IL-2 and IL-6 were going down.The biodifferences.Grade Two and Grade One(t=1.96～2.52,P＜0.05).Grade Two and the control(t=3.53～4.20,P＜0.01)were different significantly,while Grade one and the control had no obvsious difference(t=1.18～1.92,P＞0.05).Conclusion N,NE,CS of the senior hish school students in Grade Three were higher,while IL-2and IL-6 were lower than that of the Grade Two.

OBJECTIVE:To study the in vitro and in vivo bacteriostatic actions of Ganmaoreduqing oral liquid(GMRDQ) METHODS:The bacteriostatic actions of GMRDQ were observed with conventional in vitro and in vivo tests RESULTS:GMRDQ could significantly inhibit Staphylococcus aureus,?-hemolytic streptococcus,?-hemolytic streptococcus,Streptococcus pneumoniae and Hemophilus influenzae and it could protect mice from death induced by intraperitoneal injection of S aureus CONCLUSION:GMRDQ has in vitro and in vivo bacteriostatic actions

Objective: To comprehensively compare the qualities of Codonopsis Radix from the different planting areas for regionalizing the planting areas. Methods: The main traits and chemical compositions of 38 samples of Codonopsis Radix from the different planting areas in Jiuzhaigou County were comprehensively compared by SPSS 17.0 software using principal component and cluster analysis. Results: Five principal components whose accumulated variance contribution rate was over 85.604% were obtained. Based on these principal components, 38 samples of Codonopsis Radix from the different planting areas in Jiuzhaigou County were clustered into four groups by within-groups linkage cluster methods. Conclusion: Principal component and cluster analysis are reliable to comprehensively evaluate the quality of Codonopsis Radix. Significant differences among these samples from the different planting areas in Jiuzhaigou County are obvious. The standardization of cultivation techniques and application should be speeded up.

To explore the relativity between alexithymia and cognitive function in somatoform disorder patients.A total of 40 patients aged 18-65 years fulfilling the criteria of ICD-10 for somatoform disorders were recruited as research group.And 40 normal healthy subjects were selected as control group.Toronto alexithymia scale-20 (TAS-20) was employed to examine the alexithymia.And cognitive abilities screening instrument (CASI) was used to measure their cognitive ability.The 9-factor scores and total score of CASI test in patients with somatoforill disorder were significantly lower than those in the normal controls (P ＜ 0.05 or ＜ 0.01).The 3-factor scores and total score of TAS-20 test in patients withsomatoforill disorder were significantly higher than those in the normal controls (P ＜ 0.05 or ＜ 0.01).In research group,CASI scores were negatively correlated with the TAS-20 scores (P ＜0.05 or 0.01).

Purpose:To study the secretion and gene expression of Angiogenesis factors in the patients with CML and study the effect of Angiogenesis in the occurrence and development of CML. Methods:Concentration of VEGF in peripheral blood was determined by using ELISA. Myeloid tissue was extracted from all CML cases and ITP patients to detect MVD by using CD34 labelling. At the same time the level of VEGF,b-FGF were detected by using RT-PCR in both peripheral blood and myeloid cells.Results:Our results showed that the concentration of VEGF was obviously higher in the peripheral blood of CML patients(177.53?153.45 pg/ml) than in those of control group(73.12?19.82 pg/ml). The gene expression of VEGF and b-FGF were both higher than those of control group. The difference has statistical significance(P

Aim To investigate the protective effects of omapatrilat on human umbilical vein endothelial cells (HUVECs) injury induced by angiotensin Ⅱ (AngⅡ) in culture. Methods Cultured HUVECs were randomly divided into 4 groups:①control; ②AngⅡ;③omapatrilat and ④AngⅡ+omapatrilat. Lactate dehydrogenase (LDH) leakage was evaluated by spectrophotometer and cell cycle by flow cytometry (FCM); Nitric oxide (NO) was measured by colorimetry and endothelin-1 (ET-1) by radioimmunoassay. Results 10 -7 mol?L -1 AngⅡ significantly increased LDH leakage and ET-1 release, and this increase was inhibited by omapatrilat (10 -6 mol?L -1). Omapatrilat promoted HUVECs proliferation and NO release. Conclusion Omapatrilat can protect human umbilical vein endothelial cells injury induced by angiotensin Ⅱ in culture, suggesting that it may play an important role in prevention and treatment of vascular diseases.

Objective To investigate the best way for the operation of congenital microtia with deformities of external auditory meatus (EAM) and middle ear. Method To analyze retrospectively 305 cases (332 ears) of auricle reconstruction operated in the Plastic Department of Auricle, Plastic Surgery Hospital, PUMC, CAMS during June to October 2005, and 49 cases (58 ears) of reconstructions of EAM and middle ear operated in the Otolaryngology Department, General hospital of PLA from December 1988 to July 2002. Results The patients/families rested content with the reconstructed auricles operated with the 3-stage way. The hearing was improved obviously after reconstruction of the EAM and ossicle for the patients with normal stapes footplate. In those patients, for whom EAM was reconstructed first, the scar developed around the orifice which affected the skin flap for auricle reconstruction later, while in those patients, for whom the auricle was reconstructed first, wrong location of the EAM orifice happened easily. Conclusions For the patients with congenital deformities of EAM and middle ear and with the requirement of recovering the appearance and hearing function, the EAM orifice should be located first according to the results of temporal CT, then the auricle reconstruction was conducted with 3-stage method. A simultaneous reconstruction of EAM and ossicle at the third stage may ensure the improvement of both outward appearance and hearing function.

Objective To explore the best way to deal with infection of expanded skin flap during auricle reconstruction. Methods 12 cases (12 ears) of expanded skin flap infection was analyzed and treated with the method of stretching the expanded skin flap during auricle reconstruction during Jan. 2003 to Dec. 2005. Results In 12 cases (12 ears) the infection was controlled well, auricles were reconstructed smoothly and no obvious difference was detected between the infected and normal skin flaps after the reconstruction of auricles. Conclusion Stretching the expanded skin flap is a good method to deal with its infection during auricle reconstruction.

Objective To discuss X-ray, CT and MR findings of primary bone lymphoma. Methods The X-ray, CT and MR findings of 6 cases with non-Hodgkin lymphoma of bone proved pathologically were retrospectively analysed.Results The average age of 6 cases was 51 years old and the lesions all localized at long bone(especially the metadiaphysis) and pelvis. Imaging findings included: osteolytic or moth-eaten bone destruction,surrounding soft tissue mass formation which generally exceeded the extents of bony changes, the cortex bone was destructed and discontinuity.Conclusion primary bone lymphoma has prevalent age and certain imaging specificity.

Objective To study the symptoms, diagnosis, treatment and prognosis of colorectal malignant melanoma.Methods Six cases of colorectal malignant melanoma diagnosed and treated in our department between 1981 and 1996 were reviewed and underwent follow-up.Results All cases had masses that could be found near dentate line in digital examination and were black or brown in color. All patients underwent operations ,operations abdomino-perineal resection for 2 cases, post pelvic excision for 2 cases, enlarged local resection for 1 case and laporotomy for 1 case.Either lymph node metastasis or liver metastasis were found prior to or during operation. All patients died within 5～23 months after being diagnosed, with average survival time of 14.7 months.Conclusions Colorectal malignant melanoma was rare and was highly of malignant with tendency of early metastasis and poor prognosis. Radical surgery combined with chemotherapy, immunotherapy and biotherapy may be effective.