Female ; Humans ; Infant, Newborn ; Pulmonary Artery ; abnormalities

ObjectiveTo investigate the epidemiology of Lyme disease and analyze the genotypes of Borrelia burgdorferi in five kinds of host animals of ticks in four cities of Xinjiang Uygur Autonomous Region,China.MethodsA total of 814 serum samples were collected from cattle,sheep,goats,horses and dogs in Urumqi,Ili,Changji and Kashgar Cities,and determined for IgG antibody against Lyme disease. A total of 135 ticks in the above-mentioned cities were collected,from which DNAs were extracted and used as template for amplification of the 5S-23S rRNA spacer fragments of B. burgdorferi by nested PCR. The positive samples were sequenced,and the results were compared with those of 5S-23S rRNA spacer of B. burgdorferi reported in GenBank by BLAST,and a phylogenetic tree was constructed by MEGA X software.ResultsThe IgG antibody positive rates against Lyme disease in Urumqi,Ili,Changji and Kashgar Cities were 23. 6%,2. 4%,2. 7% and 0 respectively,which showed significant difference（χ2= 48. 481,P < 0. 001）. However,the positive rates in cattle,sheep,goats,dogs and horses were 1. 1%,4. 4%,18. 7%,60. 5% and 0 respectively,which showed sig-nificant difference（χ2= 129. 03,P <0. 001）. Of the 135 tick DNA samples,24 were positive for B.burgdorferi,indicating a carrier rate of 17. 78%. Phylogenetic analysis showed that the genotypes B.garinii accounted for 75%,while B.afzelii accounted for 16. 67%,and B.burgdorferi accounted for 8. 33%.ConclusionThere are Lyme diseases in cattle,sheep,goats and dogs in Urumqi,Kashgar and Ili Cities of Xinjiang Uygur Autonomous Region,China,of which the major genotype is B.garinii. The study provides a scientific basis for prevention and control of Lyme disease in Xinjiang.

DNA methylation is a common process of epigenetics,and also is the third mechanism of tumor related gene inactivation.It plays an important role in the development of cervical cancer.Studies show that methylation of p16,FHIT,IGSF and DAPK1 have relationship with cervical cancer,thus they may be a biomarker for the early diagnosis of cervical cancer.Meanwhile,advanced study of cervical cancer related gene methylation provides a new target point for treatment.

To promote the development of key discipline construction in hospitals,we described the integration of refined management into the construction of key disciplines and different aspects including personnel training,featured specialty,research,and hardware were discussed.We concluded that refined management in essential to the development of hospital,and a mechanism needs to be established to ensure the long-term effect.

Objective:To observe the effect of prolonging life and inhibiting tumor growth of Xiaozhensan on H22 cells-bearing mice and study the mechanism of action. Methods:①H22 cells-bearing mice models were made by subcutaneous injection and were divided into six groups:model group,low,middle and high dosage groups of Xiaozhensan,5-FU group,5-FU plus Xiaozhensan middle dosage group. After 10 days' treatment,the mice were killed and the anti-tumor rates were calculated,to detect the content of IL-2 and observe pathological sections of tumor and expression of Bcl-2 by immunohistochemical method. ②H22 cells-bearing ascites mice models were divided as the above. The living days of the mice were recorded,the prolongation of life span were calculated. Results:Xiaozhensan had the function of inhibiting the growth of tumor,and there was the dependability between quantity and effect. In pathomorphology,the sections of Xiaozhensan groups showed nuclear shrinkage,necrosis,hemorrhage,inflammatory cell infiltration on the periphery,etc. Xiaozhensan could increase the content of IL-2 and inhibit the expression of Bcl-2. Xiaozhensan combined with 5-FU can prolong the life span of the mice bearing ascites tumor. Conclusion:Xiaozhensan had the function of inhibiting the growth of tumor. The mechanism of action may be concerned with these aspects:enhancing the cellullar immunologic response and strengthening the ability to resist tumor,inducing apoptosis and weakening reproductive activity of tumor,etc. Middle dosage group of Xiao Zhen San combined with anticarcinogenic western medicin can prolong the life span of the mice bearing ascites tumor. This study provided dependable experimental documents for clinical application of Xiaozhensan,and had some guiding significance in clinic.

Objective To compare and determine the reproducibility of apparent diffusion coefficient (ADC)values of the normal liver parenchyma in respiratory-triggered,breath-hold,and free-breathing diffusion weighted magnetic resonance imaging (DWI). Methods 11 volunteers did three times of breath-hold (excitation number is 1,at the end of the expiration,thickness/gapping=5 mm/1 mm), respiratory-triggered (excitation number is 2,at the end of the expiration,thickness/gapping=5 mm/1 mm),free-breathing (excitation number is 4,thickness/gapping=5 mm/1 mm)and for a total of nine axial liver DWI,the gradient factor was 800 s/mm2 .Then they were all coronally reconstructed (thickness/gapping=8 mm/1 mm).The nonparametric test was used to compare all axial and coronally reconstructed liver ADC values;and the Bland-Altman method was used to evaluate the ADC values’repeatability in coronally reconstructed images.Results The breath-hold DWI’s mean ADC value was (1.09±0.07)×10-3 mm2/s significantly lower than the respiratory-triggered DWI(1.16± 0.09)×10-3 mm2/s and the free-breathing DWI(1.19±0.10)×10-3 mm2/s(P<0.005),the axial and coronal images’results were similar.The ADC values’average difference and scope of consistency of coronal image were (-0.09 to -0.01±0.09 to 0.48)for breath-hold DWI,(-0.04 to 0.01±0.11 to 0.17)for respiratory-triggered DWI,(-0.07 to -0.02±0.11 to 0.18)for free-breathing DWI,respectively.Conclusion The breath-hold DWI’s ADC value of normal liver parenchyma is lower than the respiratory-triggered and free-breathing DWI,and showed more poorer repeatability.It is suggested that the measurement of ADC values of the liver parenchyma should be done within the respiratory-triggered or free-breathing DWI.

Objective To investigate the clinical efficacy of double-plate fixation combined with bone graft on SchatzkerⅤ/Ⅵ tibial plateau fractures.Methods Totally 35 cases with tibial plateau fractures from April 2013 to October 2015 were selected into an observation group,and treated with dual plate fixation combined with bone graft.Another 35 cases with tibial plateau fractures from March 2011 to March 2013 were enrolled in a control group,and treated with conventional anatomical plate.The data of the two groups were analyzed.Results Pain scores in observation group were lower than those in the control group,and the knee joint function scores in observation group were higher than those in the control group (P＜0.05);the healing degree was 65.7％ in the control group,which is significantly less than that (91.4％) in the observation group (P＜0.05);the complication incidence rate was 8.6％ in the observation group which was obviously lower than that (34.3％) in the control group (P＜0.05).Conclusion Double-plate fixation combined with bone graft in the treatment of tibial plateau fracture shows a high efficacy,and is worthy of clinical application and promotion.

Objective] To discuss the relationship between CT perfusion imaging and TCM syndrome of liver cancer patients. [Methods]Through testing the Perfusion parameters of Lesions, surrounding liver tissue and liver tissue perfusion in the distance, we may explore the correlation among perfusion parameters, TCM syndrome of primary liver cancer, then explore the phase rule.[Results] There exists significant difference between perfusion parameters and TCM syndrome of liver cancer patients. Hepatic arterial perfusion(HAP), portal venous perfusion(PVP):deficiency syndrome of both liver and kidney yin>syndrome of heat-damp>syndrome of qi stagnation and blood stasis>syndrome of hepatic stagnation and spleen deficiency;Hepatic perfusion index(HPI):syndrome of hepatic stagnation and spleen deficiency>syndrome of qi stagnation and blood stasis>syndrome of heat-damp>deficiency syndrome of both liver and kidney yin. Child-Pugh classification: syndrome of hepatic stagnation and spleen deficiency(5.34 ±1.46),syndrome of qi stagnation and blood stasis(6.82±0.94),syndrome of heat-damp(8.34±1.12),deficiency syndrome of both liver and kidney yin(9.01±1.19).There exists significant difference between Child-Pugh classification and TCM syndrome of liver cancer patients(P<0.05). The result shows that a high positive correlation between AF, PI and Child-Pugh classification(P<0.05);There exists a negative correlation between PF and Child-Pugh classification(P<0.05).[Conclusion] CT perfusion parameters can be used as an objective indicator of middle-late stage of TCM syndrome of liver cancer patients.

Achalasia is an esophageal motility disorder with impaired relaxation of lower esophageal sphincter.Its clinical manifestations are progressive dysphagia, retrosternal pain, regurgitation of undigested food, weight loss.Medication, endoscopic treatment and surgery are the main treatments of achalasia.Peroral endoscopic myotomy (POEM) is an endoscopic mini-invasive technique which has smaller surgical injury, remarkable curative effect, lower cost, and lower recurrence rate when compared with the traditional treatment of achalasia.This article reviewed the advances and prospects of POEM in treatment of achalasia.

Refined management calls fordetail decides result,operated in health achievements transformation in hospital,which is a new try.First of all,the author expatiated upon the connotation of refined management in this paper,and then,elaborated the practice of refined management of health achievements transformation in hospital.After that,he analysed the shortage of health achievements transformation.Finally,according to the actuality of health achievements transformation in hospital,the author proposed some countermeasures,such as strengthening the application of scientific achievements and the transformation consciousness,reforming the management system,reinforcing the construction of talents rank,intensifying the construction of promotion institution of scientific and technological achievements,and enhancing the hospital and enterprise' s joint,actively absorbing research funds.