Necrotizing pneumonia is a severe complication of community-acquired pneumonia in children, with no clear deif-nition currently, characterized by parenchymal injury, necrosis and cavity formation. Streptococcus pneumonia is the most common pathogen;however a positive microbiology is not always obtainable. The clinical manifestation of necrotizing pneumonia is more seri-ous than ordinary pneumonia, and complications are common. Chest CT scan is helpful to early detection and diagnosis. Antibio-tics remain the mainstay of treatment, and the prognosis is relatively well.

Objective To study the effect of acupuncture Zusanli on brain functional magnetic resonance imaging.Methods 96 healthy volunteers were randomly divided into acupoint group and non-acupoint group.The acupoint group received acupuncture Zusanli,and the non-acupoint group received manipulation,acupuncture and sham acupuncture.The functional brain imaging was determined by MRI,the brain functional images were compared between two groups.Results All subjects were matched with the end of the experiment,the acupoint group received acupoint stimulation,had obvious needling sensation,the non-acupoint group had mild needling sensation.The acupoint group activated 13 brain regions,non-acupoint group in 10 brain regions,the thalamus,pons,supramarginal gyrus in non-acupoint group without the activation.In the basal ganglia (28 cases vs.5cases) and temporal lobe (38cases vs.21cases) between the two groups were activated,the difference was statistically significant(x2 =36.250,P ＜ 0.05).In the temporal lobe,the left hemisphere response rate(73.68％) was significantly higher than the right hemisphere (36.84％),the difference was statistically significant (x2 =8.265,P ＜ 0.05).In the basal ganglia,left hemisphere activation (67.86％) was higher than that of the right hemisphere(42.86％),but the difference was not statistically significant (x2 =0.724,P ＞ 0.05).Conclusion The research of acupuncture by brain functional magnetic resonance imaging showed that Zusanli acupoint can activate the brain function area through brain regulation and treatment of body organs,so as to prove the existence of its own and meridian sensing characteristics.

Objective: To prepare rabbit polyclonal antibody against human P-element-induced wimpy testis like 2 (HIWI2) protein, identify its properties and investigate its distribution in normal and tumor tissues by means of tissue chip. Methods: PIWIL2 peptide was synthesized chemically and conjugated to Keyhole limpet hemocyanin (KLH) as an immunogen. Then the PIWIL2-KLH conjugation was injected into rabbits subcutaneously to produce polyclonal antibodies. The specificity and sensitivity of antibodies were identified by ELISA and Western blotting after purification by affinity chromatography. PIWIL2 was then immuno-stained on the tissue chip to study its distribution in the normal and tumor tissues. Results: Rabbit's antibodies against human PIWIL2 were prepared after the injection of PIWIL2-KLH conjugation subcutaneously. These antibodies were identified as PIWIL2 peptides by ELISA and Western blotting assay. PIWIL2 protein expression was tissue-specific in tumor tissues, with PIWIL2 protein found in the cytoplasm of smooth muscle cells of most normal and tumor tissues. Conclusion: The polyclonal antibodies against human PIWIL2 protein have been successfully prepared, which provides a basis for further study on the role of PIWIL2 in the pathway of miRNA/RNA.

Humans ; Infant ; Otitis Media with Effusion ; diagnosis

Late-onset hypogonadism (LOH) is a clinical and bio-chemical syndrome associated with advancing age in males and seriously affects the quality of life of some of the patients. A classical therapeutic option for LOH is testosterone supplementary treatment (TST). Its effectiveness has been verified, whereas its long-term safety remains to be further evaluated. With deeper insights into LOH, many new therapeutic strategies have been proposed, which include the treatments with gonadotropins, testosterone precursors (such as dehydroepiandrosterone [DHEA]), non-aromatizable androgens (such as dihydrotestosterone [DHT]), antiestrogens (such as aromatase inhibitors and estrogen receptor antagonists), and Chinese medicine. Meanwhile, studies on the transplantation of Leydig stem cells, selective androgen receptor modulators (SARMs), and selective estrogen receptor beta (ERbeta) agonists have shed new light on the treatment of LOH.
Humans ; Hypogonadism ; drug therapy ; surgery ; therapy ; Male ; Testosterone ; therapeutic use

Carcinoma ; diagnosis ; Carcinoma, Papillary ; Diagnostic Errors ; Female ; Humans ; Thyroglossal Cyst ; diagnosis ; Thyroid Neoplasms ; diagnosis ; Young Adult

Objective:To investigate the protective effects and mechanism of ligustrazine on myocardial ischemia reperfusion injury(MIRI) in rats.Methods:30 Wistar rats were divided into three groups randomly:sham operation group,ischemia reperfusion injury group(IR),and ligustrazine preconditioning group(LI).The model of MIRI was produced,Immunohistochemical S-P method was used to detect the expressions of HSP25 and p38MAPK proteins in MIRI tissues.Results:Compared with IR group,the expression of HSP25 protein in LI group increased significantly(P

Objective To discuss the clinical features of dystonia manifested as Parkinsonism ( PKS) . Methods Clinical materials of a patient with dystonia manifested as PKS were analyzed retrospectively. Results The onset age of the young women was 31 years old, who was started asymmetrically with symptoms of claudication and tremor of the right foot. Levodopa had a short-term effect. The results of dopamine transporter ( DAT) PET showed that DAT in retrolentiform part were decreased significantly. Atypical Parkinson's disease was considered and she was treated as PKS long-termly. Subsequently, heterozygous mutation of c. 268-4T>A (NM_018105) in DYT6 gene was found through the next-generation sequencing, which was a kind of splicing mutation and confirmed by the first-generation sequencing. Conclusions Patients with dystonia might share similar clinical manifestations with PKS. Particularly, they should be differentiated with young-onset Parkinson's disease combined with focal dystonia. Clinical observation and genetic testing are important approaches to differentiate them.

Objective To observe the expression of neuroglobin (Ngb) in cerebral cortex of traumatic brain injury rats, and investigate the role of neuroglobin in brain injuries. Methods A traumatic brain injury rat model was constructed with Feeney's method. Fifiteen experiment animals were divided randomly into normal control group, 12h-post-trauma group and 36h-post-trauma group (5 each). Immunohistochemistry and image analysis techniques were used to measure the area, optic density (OD) value and integrated optic density (IOD) value of Ngb immunoreactive products in primary somatosensory cortex (S1) and retrosplenial granular b cortex (RSGb), which was located in the inner side and out side of traumatic brain injury cavity in coronal section of rat brain respectively. Results Ngb immunoreactive products were significantly increased after brain trauma, especially in 36h-post-trauma group. The image analysis showed that there was no significant difference of immunoreactive product area in the 3 groups (P