Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective:To prepare doxorubicin-tetrandrine complex liposomes for technology study and quality control.In order to provide a new idea to reverse the tumor multidrug resistance in clinic.Methods:The formulations of doxorubicin-tetrandrine complex liposomes were optimized by three different kinds of methods.And the optimum formula was selected according to the entrapment efficiency.Re- sults:The complex liposomes were prepared by(NH4)2SO4-gradient method combined with pH gradient method according to optimum recipe.The optimum recipe of DOX-TET liposome was founded as DOX-TET/EPC of 1∶10,EPC/Ch of 3∶1,pH value of 7.6,incubation temperature of 50℃,(NH4)2SO4 concentration of 250 mmol/L.Under the formulation,DOX and TET were encapsulated 90.77%and 80.12%,respectively.Conclusion:The doxorubicin-tetrandrine complex liposomes have high entrapment efficiency with fine looking, which provides basis for the further studies.

Objective To investigate the occurrence of hemolytic disease in neonates with maternal and neonatal blood group incompatibility,and to investigate the related factors affecting the occurrence of hemolytic disease.Methods A total of 52 newborns with maternal and neonatal blood group incompatibility in our hospital from May 2016 to May 2014 were selected as the research subjects.The clinical data of all neonates were analyzed by adopting the systematic review method,and the ocurrence situation of ABO hemolytic disease in all subjects was statistically analyzed.The differences in general data of age,gender and related clinical examination results were compared among the neonatal patients.The related factors possibly affecting neonatal ABO hemolytic disease were investigated.Results (1) Among 52 neonates,30 cases (57.69%) appeared neonatal hemolytic disease,and the incidence rate was high;(2) the non-conditional single factor Logistic regression model analysis showed that the weight,height,BMI,HGB,direct bilirubin,indirect bilirubin,total bilirubin and indirect bilirubin /total bilirubin and birth time could be the related factors affecting neonatal hemolytic disease occurrence;(3) non-conditional multiple factor Logistic regression model analysis showed that HGB,direct bilirubin,indirect bilirubin,total bilirubin and indirect bilirubin / total bilirubin and birth time were the independent influencing factors of neonatal hemolytic disease occurrence.Conclusion The incidence rate of hemolytic disease in neonates with maternal and neonatal blood group incompatibility is high,and the birth time,HGB concentration and related bilirubin expression level may be the possible influencing factors of hemolytic disease in neonates with maternal and neonatal blood group incompatibility.

Medical institutes in the United States,the United Kingdom and Canada have systematically explored and published standard of physician competence,such as ACGME six-critical-components,GMC good medical practice and CanMEDS,which has led to changes in the content,item format and clinical skill test of foreign medical incensing exam.Foreign research inspires Chinese counterparts to study and develop the Chinese standard of physician competence so as to continuously enhance the medical licensing exam.

Objective To investigate the indications and clinical values of percutaneous transthoracic biopsy guided by different locating means respectively on pulmonary lesions. Methods Patients were divided into different groups according the locating methods,which included X -ray, type -B ultrasonography, and CT. Pathologic diagnosis and complications were retrospectively analysed between groups. Conclusion Each locating method has its own advantages and disadvantages. Results we should choose the optimistic one according the patients′ condition.

Objective To analyze the clinical effect of small incision operation and traditional operation in treatment of pediatric hernia,and to provide a theoretical basis for the clinical treatment of pediatric hernia.Methods 68 children with pediatric hernia were treated as the research subjects,they were randomly divided into control group and observation group,34 children in each group.The control group adopted the traditional operation treatment,while the observation group was taken small incision.The clinical efficacy and complications of both two groups were compared. Results The operation time,bleeding volume,hospitalization time and incision length of the observation group were (10.45 ±1.35)min,(5.21 ±0.78)mL,(1.98 ±0.76)d,and (1.04 ±0.23)cm,which were better than those of the control group [(15.67 ±3.24)min,(8.56 ±1.87)mL,(3.78 ±1.12)d,and (2.54 ±0.65)cm,respectively],the differences were significant (t =5.760 0,5.747 7,4.171 7,7.986 8,all P <0.05).The incidence rate of complica-tions of the observation group was 2.94%,which was significantly lower than that of the control group (11.76%),the difference was significant (χ2 =5.7118,P <0.05).Conclusion The clinical curative effect of using small incision in the treatment of pediatric hernia is significant,this method has the advantages of small operation wound,less pain, faster postoperative recovery,less complications and other advantages,it is worthy of clinical application.

Cervical spondylotic amyotrophy is receiving increasing attention in recent years as a class of specificity of cervical spondylosis,this paper refers a large number of reference reported in the literature,and gives an accurate summary of the disease from the basic definition of disease,pathogenic factors,clinical manifestations,diagnostic criteria and prognostic point of view,and provides a theoretical foundation for clinical diagnosis and treatment of cervical spondylotic amyotrophy.

Objective:To express MSP1-31 gene of Plasmodium falciparum in Salmonella typhi CVD908 vaccine strain using a tetracycline-controlled P LtetO promoter. Methods:The MSP1-31 gene was cloned into the plasmid of pZE11 and transformed into the CVD908/tetR strain by electroporation. Expression of MSP1-31 in CVD908/tetR strain was detected using the method of Western blot. Results: The recombinant plasmid of pZE11/MSP1-31 was constructed, there was effective expression of MSP1-31 protein in CVD908/tetR strain in presence of tetracycline, and no expression of gene in absence of tetracycline. Conclusion: The recombinant Salmonella typhi strain in which the expression of Plasmodium falciparum MSP1-31 fragment induced by tetracycline is established successfully.

This paper discussed the detection of tumor micrometastasis, and analysed the influence of chemotherapy and blood transfusion on the immunity of tumor patients. Chemotherapy and blood transfusion can increase the opportunity of tumor metastasis. By analysing the mechanism of the treatment of tumor with traditional Chinese medicine, the author put forward the new method about eliminating tumor micrometastasis with traditional Chinese medicine.

Objective To study the chemical constituents from the seeds of Ziziphus jujuba var.spinosa,which is used as a sedative herbal medicine in China.Methods The constituents were separated and purified by silica gel column.Their structures were elucidated based on their physicochemical properties and spectral analysis.Results Six compounds were obtained from 95% EtOH extracts in the seeds of Z.jujuba var.spinosa.They were identified as 2?,3?-dihydroxy-lup-20(29)-en-28-oic acid methyl ester(Ⅰ),?-sitosterol(Ⅱ),betulin(Ⅲ),betulinic acid(Ⅳ),hexadexanoic acid 2,3-dihydroxypropyl ester(Ⅴ),and daucosterol(Ⅵ).Conclusion Compound Ⅰ is a novel lupane triterpene named as alphitolic acid methyl ester,compound Ⅴ is obtained from the seeds of Z.jujuba var.spinosa for the first time.