Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Medical institutes in the United States,the United Kingdom and Canada have systematically explored and published standard of physician competence,such as ACGME six-critical-components,GMC good medical practice and CanMEDS,which has led to changes in the content,item format and clinical skill test of foreign medical incensing exam.Foreign research inspires Chinese counterparts to study and develop the Chinese standard of physician competence so as to continuously enhance the medical licensing exam.

Objective:To prepare doxorubicin-tetrandrine complex liposomes for technology study and quality control.In order to provide a new idea to reverse the tumor multidrug resistance in clinic.Methods:The formulations of doxorubicin-tetrandrine complex liposomes were optimized by three different kinds of methods.And the optimum formula was selected according to the entrapment efficiency.Re- sults:The complex liposomes were prepared by(NH4)2SO4-gradient method combined with pH gradient method according to optimum recipe.The optimum recipe of DOX-TET liposome was founded as DOX-TET/EPC of 1∶10,EPC/Ch of 3∶1,pH value of 7.6,incubation temperature of 50℃,(NH4)2SO4 concentration of 250 mmol/L.Under the formulation,DOX and TET were encapsulated 90.77%and 80.12%,respectively.Conclusion:The doxorubicin-tetrandrine complex liposomes have high entrapment efficiency with fine looking, which provides basis for the further studies.

Objective To investigate the occurrence of hemolytic disease in neonates with maternal and neonatal blood group incompatibility,and to investigate the related factors affecting the occurrence of hemolytic disease.Methods A total of 52 newborns with maternal and neonatal blood group incompatibility in our hospital from May 2016 to May 2014 were selected as the research subjects.The clinical data of all neonates were analyzed by adopting the systematic review method,and the ocurrence situation of ABO hemolytic disease in all subjects was statistically analyzed.The differences in general data of age,gender and related clinical examination results were compared among the neonatal patients.The related factors possibly affecting neonatal ABO hemolytic disease were investigated.Results (1) Among 52 neonates,30 cases (57.69%) appeared neonatal hemolytic disease,and the incidence rate was high;(2) the non-conditional single factor Logistic regression model analysis showed that the weight,height,BMI,HGB,direct bilirubin,indirect bilirubin,total bilirubin and indirect bilirubin /total bilirubin and birth time could be the related factors affecting neonatal hemolytic disease occurrence;(3) non-conditional multiple factor Logistic regression model analysis showed that HGB,direct bilirubin,indirect bilirubin,total bilirubin and indirect bilirubin / total bilirubin and birth time were the independent influencing factors of neonatal hemolytic disease occurrence.Conclusion The incidence rate of hemolytic disease in neonates with maternal and neonatal blood group incompatibility is high,and the birth time,HGB concentration and related bilirubin expression level may be the possible influencing factors of hemolytic disease in neonates with maternal and neonatal blood group incompatibility.

Objective To investigate the indications and clinical values of percutaneous transthoracic biopsy guided by different locating means respectively on pulmonary lesions. Methods Patients were divided into different groups according the locating methods,which included X -ray, type -B ultrasonography, and CT. Pathologic diagnosis and complications were retrospectively analysed between groups. Conclusion Each locating method has its own advantages and disadvantages. Results we should choose the optimistic one according the patients′ condition.

Objective To observe the effect of Baduanjin,a health Qigong,on the rehabilitation of type 2 diabetes mellitus(T2DB)patients.Methods Forty-eight T2DB patients were equally randomized into two groups:group A received routine medicine and Baduanjin exercises,and group B received medicine only,the treatment lasting 24 weeks.The vital capacity(VC),body mass index(BMI),waist-hip ratio,blood glucose and blood lipid levels were detected before and after treatment.Results Compared with those before treatment, the number of patients with increased VC under conditions of standing still or being loaded was increased obviously, BMI and waist-hip ratio were decreased,total cholesterol(TC),fasting blood glucose(FBG)and glycosylated hemoglobin(GHB)levels were decreased,and high-density lipoprotein cholesterol(HDL-C)level was increased in group A after doing Baduanjin exercises(P

Objective: To observe the analgesic effect and adverse effects of Controlled-Release Oxyco-done tablets(oxycontin) on moderate and severe chronic cancer pain, and the improvement of quality of life(QOL) in the cancer patients after the treatment. Methods: A total of 72 patients with moderate and se-vere chronic cancer pain were selected .The analgesic effects,adverse effects and quality of life (QOL) were observed and evaluated. Controlled-Release Oxycodone tablets were administered at an initial dose of 5 mg or 10 mg every 12 hours according to the degree of pain. The next analgesic dose should be adjust-ed if breakthrough pain occurs more than twice in 24 hours. If the initial dose is 5 mg, it may be increased to greater than or equal to 10 mg. If the initial dose is greater than or equal to 10 mg, the dosage may increased by 25%～50%. Short-acting morphine tablets are used to control the breakthrough pain. Results: The doses ranged between 10～100mg/d .Among the 72 patients with moderate and severe chronic cancer pain, 12 (16.7%)achieved complete remission ,52(72.2%)achieved partial remission,6(8.3%) achieved minor remis-sion.The overall rate of pain relief 88.9%. The mainly adverse reactions were including, nausea and vomiting, dizziness, drowsiness and dysuria. Followed the reduced of the pain intensity ,the QOL of most cancer pa-tients was improved. The KPS of 12 patients had been obviously improved, 20 patients had mildly improved, and 40 patients were stabilized. Conclusion: Oxycodone hydrochloride controlled-release tablets are effective and safe for the management of chronic cancer patients with moderate and severe pain, with less adverse reactions, and the QOL of cancer patients were significantly improved.

Objective:To evaluate clinical application of American College of Prosthodontics classification system for complete edentulism;and to analyze the relationship between clinician's rating of general degree of difficulty of each case and patients' rating of denture satisfaction.Methods:One hundred and seven edentulous patients were examined clinically using American College of Prosthodontics(ACP) classification for complete edentulism.The least heights of patients' mandible were measured on panoramic radiographs.Clinician rated general degree of difficulty of each case on visual analogue scale.Six month following denture delivery,patients rated their denture using Mcgill satisfaction Visual Analog Scale(VAS).Multivariate linear regression analysis were conducted to analyze the relationship between clinican's rating of general degree of difficulty and mandibular bone height adjusting for confounding factors such as mandibular ridge form,soft tissue quality etc.Pearson correlation analysis was conducted to analyze the correlation between clinician's rating of case difficulty and patients' rating of denture satisfaction.Results: Advanced residual ridge resorption were found in around 80%(83/105) of all the cases.When the least mandibular bone height were measured on panoramic radiographs,intra-examiner reliability was 0.96,inter-examiner reliability was 0.90.Cases were rated as more difficult when patients showed lower mandibular bone height,unfavoriate ridge form(knife ridge and irregular ridge),mobile soft tissue and longer period of wearing time of their previous dentures.No significant correlation(r

Objective To investigate the therapeutic effect of two-stage delayed open reduction and internal fixation on Pilon fractures.Methods The clinical data of 36 patients(26 males and 10 females;aged 18-57 years with a mean of 38.5 years;9 patients suffered from open fractures and 27 with closed fractures;25 combined with fibular fractures) with monolateral Pilon fracture hospitalized in succession from Jan.2001 to Oct.2006 were analyzed retrospectively.According to the Ruedi-Allgower classification,there were 6 cases with type Ⅰ,21 with type Ⅱ and 9 with type Ⅲ fracture.All patients underwent temporary fixation with calcaneal traction or plaster slab for 7 to 14 days(mean 9.8d),and then they were treated with delayed surgery when the soft tissue injuries subsided.Ankle functions were evaluated to assess the clinical outcomes,and complications were documented for further analysis.Results Follow-up was performed for 19 to 34 months(in mean of 28 months).Based on the Mazur classification,the ankle function in 13 cases was excellent,17 as good,4 as fair and 2 as poor,with a satisfactory rate of 83.3%.Postoperative complications included 2 cases of superficial incision infections,2 of skin slough and 19 of articular degeneration.Conclusion Two-stage delayed open reduction and internal fixation is a reasonable and effective procedure in treating Pilon fractures with advantages of few complications and satisfactory ankle function.

Objective To determine the free thiols in the chimeric protein PfCP-2. 9 of Plasmodium falciparum expressed by Pichia pastoris. Methods Two experiments of reverse phase HPLC and Ellman' s reaction were applied to the PfCP-2.9 for the determination of its free thiols. For RP-HPLC analysis, three kinds of samples were tested: PfCP-2. 9, dithiothreitol-reduced PfCP-2.9 and indoacetic acid-alkylated PfCP-2.9. Results Both experiments showed that there were no any free thiols present in the PfCP-2. 9. Conclution The disulfide bonds between cysteine residues of PfCP-2. 9 were formed completely.