Objective To explore the relationship between the expression of NF-κB and VEGF and the biological behaviors of prostatic carcinoma.Methods Immunohistochemical method was used to examine the expression of NF-κB and VEGF in 40 cases of benign prostatic hyperplasia (BPH) and 53 cases of prostatic carcinoma.Results The positive rates of NF-κB and VEGF in prostatic carcinoma and BPH were 64.2％ and 69.8％,12.5％ and 37.5％ respectively.There are more cases with positive NF-κB and VEGF expression in prostatic carcinoma group than in the BPH group( x2 =24.976,9.655,P ＜ 0.01 ).The expression of VEGF and NF-κB in prostatic carcinomas increased significantly with the decrease of tumor differentiation and advance of the TNM stages(x2 =15.936,18.459;4.316,14.205,P ＜0.01 or P ＜0.05);The expression of VEGF in prostatic carcinoma was positively correlated with NF-κB ( r =0.297,P =0.027 ).Conclusion The expression of VEGF and NF-κB in prostatic carcinoma are highly correlated with each other and may be suggestive to understand the biological behavior of prostatic carcinoma.

Strengthening basic animal operation training is not only beneficial to improving students’ practice ability so as to help students lay a solid foundation for clinic treatment and research work.,but also plays an important role in physiological scientific experiment teaching,which is essential for improving teaching quality. We have an active investigation in this aspect.

The paper reviews the background and current situation of medical disputes and puts forward the concept of the reaction chain of medical disputes in view of their high occurrence rate and the difficulty in handling them. It discusses the framework, chief content, basic principles and major features of the reaction chain of medical disputes and argues that as a commonly used tool for handling medical disputes, the reaction chain renders it possible to check from the angje of the interests of the patients whether each party involved in the dispute has fulfilled its duties. Intended to improve the professional ethics of the physicians and the quality of medical care and calling on the patients to rebuild their confidence in the physicians, the reaction chain indicates that it is necessary to scientifically analyze the entire process and various influencing factors of medical disputes and bring into play the advantages of integration so as to successfully resolve medical disputes.

[Objective]To observe the effect of Homoharringtonine on Adjuvant Arthritis Rats.[Methods]Observe the effect of Homoharringtonine on Adjuvant Arthritis Rats’ SP,IL-1? and TNF-?;we take the Adjuvant Arthritis Rats as model and Tripterygium wilfordii polycoside was the positive group.[Results]The Homoharringtonine and Tripterygium wilfordii polycoside group’s level of TNF-?,IL-1? in the serum and synovial membrane decreased instructively,and their dose of SP in the plasma and synovial membrane was suppressed obviously too.[Conclusion]Homoharringtonine has the effect of anti-inflammation,it may treat the Adjuvant Arthritis Rats through reducing the dose of TNF-?,IL-1? in the serum and synovial membrane,inhibiting the secretion and releasing SP in the plasma and synovial membrane.

ObjectiveTo evaluate the efficacy of intravenous cediland(lanatoside C) and esmolol and daltiazem for controlling rapid atrial arrhythmias.MethodsA total of 94 patients with rapid atrial arrhythmias were randomized to receive intravenous cediland(n=29) and esmolol(n=30) and daltiazem(n=35),respectively.ResultThe total efficacy rate were 86%,83% and 85%,with a mean decrease in heart rate by 30.4%,29.3 and 27.6% compared to baseline,and mean response times was 34.3?21.0min、8.1?2.1min and 11.3?3.8min,respectively.ConclusionThree drugs are all effective、rapid and safe in slowing rapid ventricular rate in patients with rapid atrial arrhythmias.

Objective To establish a method for determination of the water-soluble components of Salvianolic acid B,Danshensu and Protocatechuic aldehyde in Salvia miltiorrhiza Bge. Method Chromatography was performed on Kromosil C18 (4.6 mm?250 mm,5 ?m). Mobile phase Ⅰ was methanol-acetonitrile-formicacid-water (30∶10∶1∶59),mobile phase Ⅱ was methanol-acetic acid-water (20∶1∶80),the flow rate was 1.0 mL/min,the detection wavelength was set at 286 and 280 nm,column temperature was 35 ℃. Result Determined 10 different habitats Salvia miltiorrhiza Bge.,the average content of Salvianolic acid B was 0.09%～6.81%,the average recovery was 99.64%,RSD=1.09%. The average content of Danshensu was 0.16%～0.68%,the average recovery was 98.94%,RSD=1.49%. The average content of Protocatechuic aldehyde was 0.01%～0.09%,the average recovery was 98.96%,RSD= 1.66%. Conclusion The method is accurate and reliable with good reproducibility,and can be used as determination standard for Salvia miltiorrhiza Bge.

Laboratory animal science teaching has become an indispensable part of medical education.This paper summarized teaching characteristics of laboratory animal science at different levels, including the curriculum setting background, curriculum arrangement as well as assessment methods, in order to provide some references for the teaching staff of laboratory animal science.

Objective To discuss the clinical features and treatment methods of chronic hydrocephalus after traumatic subarachnoid hemorrhage.Methods A total of 40 cases of chronic hydrocephalus after traumatic subarachnoid hemorrhage were analyzed retrospectively.Results Eight cases improved after treatment of non-operation,the lateral ventricle external drainage was done in 32 cases of chronic hydrocephalus.Shunt tube obstruction occurred in 3 patients,with abdominal,subcutaneous and intracranial infection.The shunting operation device was pulled out and the 3 patients received operation for the second time.Two cases appeared intracranial hemorrhage,and the hemorrhage was absorbed after conservative treatment.Total Glasgow prognosis score was good in 32 cases,with mild disability in 5 cases,moderate disability in 2 cases and severe disability in 1 case.Conclusions Traumatic subarachnoid hemorrhage after chronic hydrocephalus should be early diagnosed and early treated,CT and MRI scan is the effective means to the diagnosis and differentiaion.The lateral ventricle external drainage is good method in treatment.

[Objective]To discuss the causes and preventive measures of early complications after using Vertes technique in treating dens axis fracture with atlantoaxial dislocation.[Method]Eighteen cases performed Vertex technique were included in this study.The early complications(postoperative or at surgery) included vertebral artery injury(2 cases),logagnosia(1 case),anopsia(1 case),hematorrhea(1 case) and wrong instrumentation(1 case).The causes of these complications and how to prevent were analyzed.[Result]All these complications were treated in time,and no sequela was found.[Conclusion]The early complications of Vertex technique in treating dens axis fracture with atlantoaxial joint dislocation can be prevented.The timely treatment must be performed when it occurs,and the effect would be satisfactory.

[Objective]To evaluate the value of ECT in diagnosing and treatment the spinal tuberculosis.[Method]ECT by injecting 99Tcm-MDP to human body was used to know the developing of focus in spinal tuberculosis and whether other bone and joints tuberculosis were complicating simultaneously.[Result]The developing of 175 patients were positive,including multi-vertebral bodies tuberculosis 146 cases,which were composed of 2 vertebral bodies 78 case,3 vertebral bodies 26 cases,4 vertebral bodies and more 42 cases.Complicating tuberculosis of bones and joints 22 cases,articulatio sacroiliaca 29 cases.[Conclusion]ECT plays an important role in diaognosing the spinal tuberculosis and the tuberculose focus of body can be found and has the guidance for doctors to formulate the therapeutic regimem of spinal tuberculosis.