3.Fiber post and its key point of application.
Chinese Journal of Stomatology 2011;46(7):442-445
8.Effect of 5-Aza-CdR on proliferation and PRDM1α gene expression in Burkitt ' s lymphoma cell line NAMALWA
Journal of Leukemia & Lymphoma 2012;21(10):607-610
Objective To explore the effect of DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-Aza-CdR) on proliferation and tumor suppressor gene PRDM1α expression in Burkitt's lymphoma cell line NAMALWA. Methods MTT was used to study the impact of DNA methylation inhibitor on proliferation in NAMALWA cells,and comparative real-time reverse transcription-polymerase chain reaction (RQ-PCR) with SYBR Green assay was used to detect PRDM1α gene expression. Results MTT results showed that 5-AzaCdR inhibited proliferation of NAMALWA cells in a dose-dependent manner.After treated with 5-Aza-CdR for 72 h at 0.01,0.0625,0.1,0.125,0.25,0.5,1,2 and 4 μmol/L,the inhibition rates were 21.93 %,39.23 %,47.69 %,50.37 %,53.54 %,57.72 %,62.31%,65.68 %,67.87 %,respectively,and the differences of absorbance among the groups were also statistically significant (all P< 0.01). Meanwhile, 5-Aza-CdR could induce the re-expression of PRDM1α, and △Ct of 0.5, 1, 2 μmol/L groups showed significant differences compared with control group (all P < 0.05). Conclusion DNA methylation inhibitor 5-Aza-CdR can significantly inhibit the proliferation of Burkitt' s lymphoma cell line NAM ALWA,and the possible mechanism of this inhibition may be induction of demethylation and re-expression of PRDM 1α.
9.Application of 18F-fluorothymidine PET/CT in diagnosis of tumor
Chinese Journal of Interventional Imaging and Therapy 2009;6(4):388-391
18F fluorodeoxyglucose, as a kind of tracer, has its limitations in positron emission tomography/computed tomography (PET/CT). 18F-fluorothymidine (18F-FLT) is a kind of new tracer. The value of 18F-FLT in diagnosis of tumor, tumor staging and assessment of therapeutic effects was reviewed in this article.
10.The clinical meaning of 1h-plasma glucose cutoff value during OGTT in the screening of diabetes and IGR
Chinese Journal of Diabetes 2010;18(3):204-206
Objective To determine 1 hour plasma glucose cutoff value during OGTT to screen diabetes and IGR and its relationship with islet β cell function and insulin sensitivity. Methods 4731 subjects were recruited to perform an OGTT.The cutoff value of 1h-plasma glucose during OGTT was analyzed by receiver operating characteristic curve (ROC curve).And according to the two cutoff values,we classified all the subjects to three groups,and compared HOMA-IR,HOMA-β,insulinogenic index and shape index among groups,then analyzed its correlation with each parameter. Results According to ROC results, to diagnose type 2 diabetes mellitus,the optimal cutoff value of 1h plasma glucose during OGTT was 12.935mmol/L,with sensitivity of 81.52%,specificity of 86.94%. To diagnose impaired glucose regulation(IGR),the optimal cutoff value of 1h plasma glucose was 9.815mmol/L,with sensitivity of 74.96% and specificity of 77.86%. We classified all the subjects to three groups, and among the three groups the insulinogenic index, shape index, HOMA-IR, HOMA-β showed statistically significant difference (all P<0.01). There was a negative correlation of OGTT-1hPG with insulinogenic index and HOMA-β(all P<0.01) and a positive correlation of OGTT-1h PG with shape index and HOMA-IR (all P<0.01). Conclusions To diagnose type 2 diabetes mellitus,the optimal cutoff value of 1h plasma glucose during OGTT is 12.935mmol/L.To diagnose impaired glucose regulation(IGR),the optimal cutoff value of 1h plasma glucose is 9.815mmol/L.And 1h PG can reflect the islet β-cell function and insulin resistance.