Objective To analyze projects funded by NSFC and SCI paper publication in orthopedic research field (Code:H06) from 2010 to 2016,the current research status and development trend in this field were reviewed and discussed.Methods The data of the projects and funding subsidized by NSFC in orthopedic research field from 2010 to 2016 were collected and analyzed;the papers published were collected by searching in the Web of Science core collection by orthopedics as keyword (article included,conference abstract and book chapters excluded;data collected by December 25,2016) and compared for analyzing the global and China's SCI papers publication in orthopedic field and the role of NSFC in it.Results During the period of 2010 to 2016,1603 projects were funded by NSFC in orthopedic field and the total subsidy fund reached ￥ 730 850 000 (direct funding reported in 2015 and 2016);821 projects of General Program were funded and subsidy fund amount to ￥481 490 000;567 projects of Young Scientists were funded ￥ 118 130 000;130 projects of Fund for Less Developed Regions were funded ￥ 56 040 000;12 projects of Key Program were funded ￥ 33 560 000;3 projects of Distinguished Young Scholars were funded ￥ 7 500 000;5 projects of Outstanding Young Scholars were funded and funding up to ￥ 5 900 000.In the past seven years,the volume of SCI papers published in the world exceed 1 million per year and the papers from the orthopedics field accounted for 0.7％ to 0.8％,ranked 54.The number of SCI papers published by Chinese institutions in the field increased year by year and country ranking climbed to No.3 in 2016 from No.8 in 2010.Among these SCI papers from Chinese institutions,the number of the papers from NSFC grants also increased year by year,ranked Top One in Chinese founding agency for 7 consecutive years.Conclusion In recent years,with the increasing of NSFC budget,the orthopedic research has been developed rapidly,but it still needs to be further strengthened in the advanced talents and high-level research.

Carcinoma, Hepatocellular ; therapy ; Catheter Ablation ; Chemoembolization, Therapeutic ; Combined Modality Therapy ; Humans ; Liver Neoplasms ; therapy ; Liver Transplantation

Carcinoma, Hepatocellular ; surgery ; Hepatectomy ; methods ; Humans ; Liver Failure ; diagnosis ; prevention & control ; therapy ; Liver Neoplasms ; surgery ; Postoperative Complications ; diagnosis ; prevention & control ; therapy

The screw holes of anatomic plate are oval, and longitudinal axis of the screw hole is parallel to the axis of the plate. The plate surrounding the screw hole is smooth, parallel to the longitudinal axis of the long bone. Under outside force or inside force, the top of screw can move on the smooth surface surrounding the screw hole; the movement is a reversible one. Domestic plate can fix bone fracture stably, and the outside force and inside force can result in micromovement of the fracture ends, stimulating the fracture ends and bone callus formation. Repetitive force is beneficial to bone callus response, protect fragile new vessels and bone trabecula from damage, and promote fracture healing.

Objective To study the effect of substance P (SP), calcitonin gene-related peptide (CGRP) on the blood flow and the function of endolymphatic sac (ES) in guinea pigs to clarify the pathogenesis of some inner ear diseases further.Methods The guinea pig model of superior cervical ganglion (SCG) resection was set up. The ESs of the model animals were stained by SP and CGRP immunocytochemically. The staining images were analysed by the computer. The ESBF was measured with the biologic microphere method.Results Compared with the control group, the staining of SP decreased sharply in the model group, however, the staining of CGRP remained unchanged. In the model animals, the ESBF was decreased either.Conclusion SP and CGRP could take part in the regulation of ESBF to affect the function of ES further.

Objective To evaluate serum TPSA and F/T ratio in the differential diagnosis of BPH and PCa. Methods Serum TPSA,FPSA and F/T ratio in 177 cases of BPH and 60 cases of PCa were assessed and evaluated both in patients within and outside the "gray zone". Results TPSA and F/T ratio were both significantly discriminating between benign and malignant diseases (0.010.05) whereas F/T ratio could ( P 0.05). Conclusions Serum TPSA is a prostate carcinoma marker,F/T ratio being an adjuvant marker of TPSA of importance on the differential diagnosis in patients within the "gray zone".

Switching Mechanism At 5′ end of the RNA Transcript(SMART)is a technology used in biology researching,So far,there is no review only about SMART technology.So,the aim is to investigate the research developments of principles,methods and applications of the SMART technology.Based on some researches and combined with the review of the related literature at home and abroad,it analyzed and evaluated the latest development of the research on SMART.As the applications of the SMART technology expand in many fields day after day,it has been proved that the SMART technology is a very useful and efficient skill to construct full length cDNA library.As more and more researchers know this technology,the advantages of the SMART technology become obvious,and meanwhile the disadvantages of the SMART technology also show up.That is to say,the technology needed to be improved.

Objective To investigate the expression of CXC chemokine receptor 4 (CXCR4) in clear cell renal cell carcinoma (ccRCC) and its relationship with the clinical pathological parameters of ccRCC.Methods The expression of CXCR4 was detected by immuno-histochemistry method in 63 cases of ccRCCs,20 cases of para-carcinoma tissues and 20 cases of normal renal tissues.The correlation between expression level of CXCR4 and clinical pathological parameters of ccRCC patients were analyzed,and the clinical significance of its expression in ccRCC was evaluated.Results The positive expression rate of CXCR4(49.2％) in ccRCCs was significantly higher than that in para-carcinoma tissues (15％) and normal renal tissue (10％),and the difference was statistically significant (P ＜ 0.05).The expression level of CXCR4 and the clinical stage and pathological grade of ccRCC were correlated (P ＜ 0.05),and was associated with lymph node transfer (P ＜ 0.05).The CXCR4 negative group overall survival rate [55.2％ (16/29)] and the average survival time(46 months) was significantly better than the positive group [38.5％ (10/26),32 months;P ＜ 0.05].Conclusions The expression level of CXCR4 in ccRCC is correlated with the clinicopathological parameters and prognosis.CXCR4 is expected to be an important marker for diagnosis and prognosis evaluation of renal cell carcinoma.

Objective To study the chemical composition from leaves of Smallanthus sonchifolius.Methods Some chromatography methods were used in the isolation procedure,while the structures were determined on the aids of NMR and MS spectral analyses.Results A new compound,together with five known compounds,was isolated from the ethanolic extract of the leaves.The new compound is characterized as 5,8-dihydroxyl-(5H,8H)-?-ionol(Ⅰ).Other compounds are obtained for the first time from the title plant and identified as ent-kaurane-3?,16?,17-triol(Ⅱ),entkaurane-16?,17-diol-19-oic acid(Ⅲ),3,4-dihydroxybenzaldehyde(Ⅳ),1-pentacosanol(Ⅴ),and 1-octacosanol(Ⅵ),respectively.Conclusion Compounds Ⅰ-Ⅵ are isolated from the plants of genus Smallanthus for the first time.Compound Ⅰ is a ?-ionol derivate firstly isolated from genus Smallanthus.It is named as sonchifolol.

Clavicular crook plate is designed based on anatomic features of acromioclavicular joint,which matches clavicle appearance,and well attaches the shaft.Not passing through the joint,the internal fixation exhibits many advantages,such as convenient usage,reliable fixation and micromovement of fixed acromioclavicular joint.So the patients could move the joint early and avoid osteoarthritis.Allogenous tendon has abundant sources,simple sampling process,and short repairing duration and can reduce injury to patients.Cryopreserved allogenous tendon has low antigenicity and immunological refection,good histocompatibility.Reconstructed coracoclavicular ligament by allogenous tendon has good material intensity,and can meet the requirements of local stress,better substitute integrity and function of coracoclavicular ligament.Moreover,it has precise effect and simple operation.It acts as long-term fixator following plate removal,thus is important supplement of clavicular crook plate fixation.Clavicular crook plate fixation in combination with allogenous tendon transplantation reconstructs acromioclavicular joint stability,effectively provides early mechanical stability and subsequent biological stability,and maximizes restoration of acromioclavicular joint structure and function.