The latest developments of techniques that prolong the half-life of protein drugs were reviewed.The therapeutic potential of several proteins is limited by their rapid clearance from the body,resulting in the need for frequent treatment of individuals to maintain drug levels.Several techniques including chemical modification,protein fusion,microsphere,glycosylation,and protease-resistant variants,and so on,prevent rapid degradation of such drugs and prolong their half-lives in vivo.Several successful protein drugs with prolonged half-life have already been on the market or on clinical trials.The principles,efficiencies and scopes of application of these techniques were described,compared and discussed.

BACKGROUND: In the ischemic cerebral area there are viable tissues of low blood perfusion. Theses tissues can be rescued as long as blood supply is restored timely. As an imaging agent,99Tcm-HL91 can be used to demonstrate the viable cerebral tissues in hypoxic and ischemic area.OBJECTIVE: To investigate the value of 99Tcm-HL91 imaging in assessing ischemic stroke,in order to provide evidences for early intervention.DESIGN: An observational and controlled trial based on patients and healthy voluinteers.SETTING: A nuclear medicine department in a university and a neurological department and a nuclear medicine department in a provincial hospital.PARTICIPANTS: From March 2000 to September 2001,eighteen inpatients and outpatients suffering from ischemic cerebral diseases and 4 volunteers were enrolled in the study.METHODS: In the 18 patients,11 were clinically diagnosed as cerebral infarction,5 TIA,2 vertebrobasilar insufficiency. All the subjects underwent 99Tcm-HL91 cerebral hypoxia tomographic imaging. The labeled 99Tcm-HL91(555-1 110 MBq) was intravenously injected into the human body and imaging was conducted after 20 -30 minutes,17 patients underwent CT or MRI examination at the same time. Eleven patients underwent 99Tcm-ECD tomopraphic imaging on the next day. The results of the 3 examinations were compared with each other. The uptake in the inside and around-infarct areas was determined by direct visual observation on the images. Positive change was given by presence of increased uptake and negative by absence. The uptake change that could not be clearly defined as positive or negative was considered critical level. But the uptake on both hemispheres was compared with ROI technology. The change over 25% between hemispheres was set as positive and the other was negative. So there was no critical group in this study.MAIN OUTCOME MEASURES: The results of cerebral hypoxia imaging,CT,MRI and cerebral perfusion imaging.RESULTS: Among the 18 patients,5 were positive for hypoxia imaging(4infarction and 1 vertebrobasilar insufficiency). In the 99Tcm-ECD perfusion,6out of 11 patients manifested of regional decreased blood perfusion. Nine patients manifested of abnormal CT or MRI results. No abnormalities were detected in the 4 volunteers.CONCLUSION: Although 99Tcm-HL91 imaging for diagnosis of cerebral ischemic disease is affected by many factors,it can identify whether the ischemic tissue is hypoxic or necrotic when the perfusion imaging demonstrate low flow. So it helps in guiding rehabilitation intervention and foretelling prognosis.

Objective To determine the curative effect of a trigeminy treatment including gen-tamycin, albumin and local oxygen therapy on treatment of ulcerative bedsore. Methods 50 cases of hospitalized patients in our hospital with ulcerative bedsore during January,2005 to December,2007 were selected. The patients were randomly classified into the experimental group and the control group with 25 patients in each group. The patients in the experimental group were treated by trigeminy treat-ment after debridement, while the patients in the control group were treated by covering with gen-tamycin and bandaging with dressing after debridement.The therapeutic effect of the two groups was compared by χ2 test. Results After the treatment, 6, 10, 6 and 3 patients in the experimental group, were healing, excellence, utility and inefficacy, respectively. On the contrast in the patients of the con-trol group, 2, 8, 5 and 10 were judged as healing, excellence, utility and inefficacy, respectively. The experimental group showed better effect than the control group. Conclusions The trigeminy treat-ment is able to remarkably increase clinical treatment effect of ulcerative bedsore.

Objective To study effects of classical intrafascial supracervical hysterectomy(CISH) on immune functions.Methods A total of 60 women with benign gynecopathy requiring a hysterectomy were randomly divided into two groups with 30 patients in each group,receiving either CISH or abdominal hysterectomy(AH).The white blood cell(WBC) count and neutrophil granulocyte percentage before and 1 day after the surgery,and peripheral T-lymphocyte subgroup(CD_3,CD_4,and CD_8),(interleukin-2)(IL-2),and interleukin-10(IL-10) 1 day before and 1 and 4 days after the surgery were measured,respectively. Results Postoperative levels of CD_3 and CD_4 in both groups decreased significantly at 1 day after operation(CISH Group: q_(CD3)=6.033,(q_CD4=4.763);AH Group: q_(CD3)=11.043,q_(CD4)=9.202;P0.05),and were significantly higher(q_(CD3)=6.531,q_(CD4)=5.269,P0.05) in the CISH Group.The IL-2 levels were significantly lower at 1 and 4 days after operation than before the operation in the AH Group((q_(1-2)=11.187),q_(1-3)=5.404,P

Adolescent ; Adult ; Cholecystitis ; complications ; drug therapy ; surgery ; Cholecystolithiasis ; complications ; drug therapy ; surgery ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Pancreatitis ; drug therapy ; etiology ; surgery ; Phytotherapy ; Postoperative Period ; Sphincterotomy, Endoscopic

Objective:To observe the effect of tuina manipulations on blood pressure and its variability in hypertension patients. Methods:Forty hypertension patients were randomized into an observation group and a medication group, 20 cases in each group. The observation group was intervened by tuina manipulations of kidney-tonifying blood-circulating and collaterals- unblocking in addition to regular medication, while the medication group was by the same medication. The 24-hour blood pressure monitoring was performed before intervention and after 3-month intervention. The blood pressure and its variability were observed and compared. Results:There were no significant differences in comparing the blood pressure and blood pressure variability between the two groups before intervention (P>0.05); after 3-month intervention, the blood pressure and its variability were significantly improved in both groups (P<0.05); the improvements in the observation group were more significant than those in the control group (P<0.05). Conclusion:Tuina manipulations of kidney-tonifying blood-circulating and collaterals-unblocking plus medication can produce a better effect than regular medication in promoting blood pressure and its variability, and this method is worth applying in clinic as it’s easy-to-operate and has no adverse effect.

[Abstract ] Objective The tension-free vaginal tape is the main method for treatment of stress urinary incontinence (SUI). The aim of the present study was to analyze the effect and safety of tension-free vaginal tape ( TVT) and tension-free vaginal tape-obtu-rater ( TVT-O) for female stress urinary incontinence and to sum up the experiences . Methods Clinical data of 142 patients who re-ceived anti-incontinence surgery ( TVT/TVT-O ) between January 2011 and May 2014 were retrospectively analyzed , 111 cases re-ceived TVT and 31 cases received TVT-O.The effect and the complication were analyzed at least 6 months post surgery .Incontinence quality of life ( I-QOL) and organ prolapse/urinary incontinence sexual function questionnaire ( P-ISQ) were used to observe the sub-jective symptoms .One hour pad test value was used to assesse the objective response . Results The effective rate post-TVT/TVT-O was 88.03%, and the cure rate was 95.07%6 months post surgery.In the group of TVT, I-QOL and P-ISQ score were 52.1 ±16.8 and 60.1 ±36.5 at time of pre-operation, and they were 92.1 ±17.1 and 62.2 ±37.8 at time of post-operation, respectively.In the group of TVT-O, they were 61.2 ±15.8 and 57.1 ±36.8 at time of pre-operation, and they were 91.3 ±20.1 and 62.1 ±35.9 at time of post-operation, respectively.Compared with pre-operation, I-QOL and P-ISQ score were obviously rised at time of post-operation ( P<0.05 ). The total incidence rate of surgery complication was 21.83%.And,in proper was urinary tract infection (16.9%),OAB (14.08%), bladder injury(11.26%), retention of urine(7.74%), nerve pain(2.81%), retropubic hematoma(1.41%), urethral injury(1.41%).All patients′surgeries were successful except three cases.One patient was cured after reoperation .The other two was improved with dislodge sling urinary incontinence . Conclusion TVT/TVT-O was a highly effective and safe method in the treatment of SUI .But we should not ignore the complications;the timely and effective treatment measures could improve the operation rate of success .

Objective To investigate the roles of high-resolution magnetic resonance imaging (HR-MRI) in the diagnosis and treatment of vertebrobasilar dissecting aneurysms (VBDA).Methods From January 2016 to December 2016,62 patients with VBDA admitted to Xuanwu Hospital,Capital Medical University using 3.0 T HR-MRI (3D-TOF-MRA,3D-T1WI,and 3D-T1WI+C sequence) and diagnosed by DSA were analyzed retrospectively.They were divided into either an asymptomatic group (n=18) or a symptomatic group (n=44) according to their medical histories.Their clinical features,imaging features,and the relationship with prognosis were analyzed.Results HR-MRI revealed that the double lumen sign/intimal flap accounted for 27.8% (n=5) and 54.5% (n=24) respectively in the asymptomatic group and symptomatic group.The intramural hematoma accounted for 22.2% (n=4) and 43.2% (n=19) respectively in both groups.The enhancement of aneurysmal wall accounted for 33.3% (n=6) and 77.3% (n=34) in the asymptomatic group and symptomatic group.There were significant differences in the above features between the two groups (all P<0.05).In the symptomatic group,18.2% (n=8) of patients had aneurysm recurrence,which was significantly higher than the asymptomatic group (5.6%,n=1).The difference between the two groups was statistically significant (P<0.05).Conclusions HR-MRI can better reveal the lesion characteristics of VBDA.The rates of aneurysmal wall enhancement and recurrence in symptomatic VBDAs are higher,which indicates that HR-MRI can assess the stability of the aneurysmal wall and identify the prognosis to a certain extent.

Objective:To investigate the efficacy and safety of different doses of tirofiban combined with coronary artery intervention in treatment of Non ST-segment elevation acute coronary syndromes (NST-ASC).Methods:110 cases with NST-ACS from October 2014 to January 2016 in our hospital were chosen and divided into the all dose group and half dose group.The TIMI blood grade before and after treatment,cardiac function before and after treatment for 30 d,major adverse cardiac events and bleeding events,hospitalization expenses and hospitalization days were recorded and compared between two groups.Results:Compared with before treatment,the TIMI 2 grade and 3 grade were obvious decreased,and the left ventricular end diastolic volume (LVEDV),left ventricular end systolic volume (LVESV) after treatment for 30 d were all obvious decreased,while the left ventricular ejection fraction (LVEF) were significant increased,P＜0.05.And the TIMI blood flow grading,LVEDV,LVESV and LVEF before and after treatment in two groups had no significant difference (P＞0.05),and the major adverse cardiac events and hospitalization days of two groups had no significant difference,P＞0.05.While the bleeding events and hospitalization expense of all dose group was obvious higher than those of half dose group,P＜0.05.Conclusions:The half dose group of tirofiban combined with coronary artery intervention in treatment NST-ACS has obvious efficacy,it can decrease the bleeding events and hospitalization expense.

China ; epidemiology ; Humans ; Occupational Diseases ; epidemiology