Objective To design and establish a set of diffusion optical tomography(DOT) system.Methods Near-infrared(NIR) laser was employed as the light source and only one photomultiplier tube(PMT) as the detector.An optical multiplexer was used to alter the detector channels.The 32 channels of the system,which are consisted of 16 launch channels and 16 detector channels,worked under the continuous-wave(CW) model and were used to acquire 256 boundary data.Results Experiments were performed based on the proposed imaging system.The intralipid was used as the tissue-like medium and the India ink as the absorber.Two sets of data on the boundary were sampled,respectively,before and after the absorber was embedded inside the tissue-like medium.The two sets of data were normalized and then used to reconstruct the absorption coefficient distribution.The recovered image reflected the real location and size of the absorber.Conclusion The proposed imaging platform can image the tissue optical parameters effectively.However the resolution of the reconstructed image was not high because the inverse problem was gravely underdetermined and the noise was not considered in the reconstruction algorithm.The recovered result in the next generation system could be improved by making more use of prior information and enhancing the performance of the system.

OBJECTIVE:To test and verify the function of Composite Radix Sophora Flavescentis injection in immunologic enhancement.METHODS:A total of 121 patients with lung cancer were randomly assigned to 2 groups:the treatment group(n=61)received common chemotherapy for 6wk plan in combination with Composite Radix Sophora Flavescentis injection(20mL,iv gtt)qd for the first 10 to 14 days,while the control group(n=60)received common chemotherapy alone for 6wk.The indexes of immunity in both groups were detected before and after treatment.RESULTS:The index of immunity in the treatment group increased significantly as compared with before treatment(P

Aim To investigate the effects of propafenone on myocardium inotropism and explore it’s possible mechanism in isolated papillary muscle of guinea pig. Methods Developed tension (DT), maximum rate of contraction (+dT/dt_ max) and maxi-mum rate of relaxation (-dT/dt_ max) were measured during propafenone perfusion before and after administration of L-type calcium channel blocker, nicardipine and selective Na+/Ca 2+ exchanger inhibitor, KB-R7943. Results ①At concentration of 0.1,1,10,30 ?mol?L -1,propafenone attenuated DT from control (0.18?0.05) g to (0.14?0.03), (0.12?0.03), (0.08?0.02), (0.05?0.02) g respectively (P

Objective:Our purpose was to establish an ideal chronic pressure-afterload heart failure rat model which has the transition from cardiac hypertrophy to heart failure. Methods: Chronic pressure-afterload heart failure rat model was induced by gradually constricting the ascending aorta of young rats. Young rats were randomly divided into 2 groups: the constricted and sham-operated groups. Clinical manifestation, tail-cuff blood pressure, organ weight, and hemodynamic data were observed at various time after operation. Results: The overall survival rate was 87%. Tail-cuff pressure began to increase in 4 weeks after operation. Left ventricular hypertrophy appeared in 12 weeks and heart failure in 5 months. Conclusion:It's a practical and reproducible model of cardiac hypertrophy that progresses to chronic heart failure.

Objective To explore relationships of expression of hypoxia?inducible factor?1α(HIF?1α), vascular endothelial growth factor(VEGF)and protein kinase B(P?Akt)with angiopoiesis and cell apoptosis. Methods Biopsy specimens were collected from skin lesions of 32 patients with lichen planus and normal skin of 20 patients with lipomyoma, and subjected to paraffin embedding. Immunohistochemical staining was performed to measure expression of HIF?1α, VEGF and P?Akt, and TUNEL technique was used to detect apoptosis of keratinocytes in these paraffin?embedded tissue sections. Microvessel density (MVD)was assessed by counting CD34?labeled vascular endothelial cells. Results HIF?1α, VEGF and P?Akt were moderately or strongly expressed in lichen planus lesions, but absent or weakly expressed in normal skin of controls, and the expression of HIF?1α, VEGF and P?Akt was significantly higher in the lichen planus group than in the control group (all P < 0.01). HIF?1α was mainly expressed in nuclei of keratinocytes, while VEGF and P?Akt were expressed in the cytoplasm of keratinocytes. In addition, the lichen planus group showed significantly increased MVD(21.27 ± 6.54 vs. 10.26 ± 1.10 microvessels/high?power(200 ×)field, t = 5.607, P < 0.01)and apoptosis rate of keratinocytes(72.81% ± 9.234% vs. 28.16% ± 3.464%, t = 8.431, P < 0.01) compared with the control group. Pearson correlation analysis showed that there were positive correlations between HIF?1αand VEGF expression, between VEGF and P?Akt expression, and between P?Akt and HIF?1αexpression in the lichen planus group(r=0.625, 0.453, 0.455, respectively, all P<0.01), and expression of HIF?1α, VEGF and P?Akt was all positively correlated with MVD(r=0.721, 0.646, 0.671, respectively, all P<0.01). Conclusion HIF?1αand its downstream target genes VEGF and P?Akt may play a certain role in the occurrence of lichen planus.

Objective To investigate the influence of endoscopic retrograde cholangiopancreatography (ERCP) on life quality of patients with common bile duct stones. Methods The life quality of 35 patients, who underwent ERCP because of common bile duct stones, were assessed by GIQLI questionnaires before, 2 and 6 weeks after the procedure. Results The mean GIQLI score of patients before ERCP was 99. 9, which was significantly increased to 112. 2 and 121.9 at 2 and 6 weeks after ERCP (P ＜0. 01 ). At 6weeks after ERCP, the GIQLI score was similar to that of normal population. Conclusion ERCP can improve the life quality of patients with common bile duct stones.

[Objective] To study the role of scaffold internal three dimensional structure of on vascularization of ?-TCP biomaterials in vivo.[Method]Twenty four adult rabbits were selected for operation.Two different three-dimensional structure ?-TCP biomaterials(wafer ?-TCP,the pore diameter was from 400 ?m to 500 ?m,the pore inter-connection diameter was 120 ?m;granulation ?-TCP,the particle diameter was from 100 ?m to 200 ?m)were implanted separately into fascia lumbodorsalis of every rabbit.The specimens were harvested in 1,2,4,8 weeks after surgery for histology,scanning electronic microscope(SEM)and SPECT studies in order to observe the vascularization of two different structure ?-TCP biomaterials in vivo.[Result]The biocompatibility of two different ?-TCP biomaterials was favourable.Only a few of immature blood capillaries were detected in some peripheral pores of two different biomaterials in one week after surgery.In four weeks of implantation,the result of histology indicated that the wafer artificial bone had vascularized completely.The number and lumens of blood vessel had increased.The peripheral blood vessel had been mature,showing vascularization crest-time.In eight weeks after sugery,there was no more increase of the number of blood vessel,while the lumens of blood vessel had increased.The mature capillaries were observed by chance.To compare with the wafer artificial bone,the vas cularization rate of the granulation artificial bone biomaterials was slower,and the number of blood vessel was less.On the other hand,the smaller lumens diameter and the infant structure existed in most of blood capillaries.Many blood vessels were not mature in four weeks,the vascular occlusion in some pores was detected.[Conclusion]The pore interconnection pathway in scaffolds is a key factor for vascularization.In other words,the higher density of pore interconnection pathway can induct more complete vascularization in scaffolds,and the diameter can restrict the lumens of blood vessel diameter.

Objective To investigate the efficacy of sorafenib alone or combination with transarterial chemoembolization(TACE)and percutaneous local cryotherapy(PLCT)for advanced hepatocellular carcinoma patients without operation opportunity. Methods Sixty-four advanced hepatocellular carcinoma patients were selected as our subjects,who were underwent treatment of sorafenib alone or combination with TACE and PLCT. Thirty-two cases with sorafenib therapy were served as sorafenib group and another 32 cases with sorafenib in combination with transarterial chemoembolization and PLCT were served as combination group. All patients were followed up for 6 - 32 months. The treatment efficacy and tumor development were recorded. Results All surgeries of the patients were succeed and no death or serious operation complications occurred. Of 64 patients, 11 were achieved a complete remission( CR),31 cases with partial remission( PR),14 cases with stable development(SD),and 8 cases with progressive disease(PD). In the sorafenib group,3 cases were with CR,11 patients with PR,12 with SD,and 6 patients with PD. In the combination group,8 patients were with CR,20 patients with PR,2 patients with SD and 2 patients with PD,and the difference was significant between the two groups(χ2 = 14. 028,P = 0. 003). The median periods to tumor progression were 20 and 53 weeks in the sorafenib group and the combination group,and the difference was significant( χ2 = 14. 773,P = 0. 000). Conclusion For hepatocellular carcinoma patients without operation opportunity,sorafenib combined with TACE and PLCT can increase the tumor remission rate and prolong the periods to tumor progression in patients with hepatocellular carcinoma.

Objective To investigate the clinical effect of hysteroscope combined with B-ultrasound in the treatment of uterin cavity adhesion. Methods Hegar dilatior or electric knife of hysteroscope was used to cut adhesion, which was monitored by B-ultrasound. Antibotics, artificial hormonal cycle treatment and IUD in uterin cavity were used after operation. Results 55 cases of uterin cavity adhesion were separated completely and had no complication. Amenorrhvea or rare menstrual flow occurred in 48 cases,and menstrual flow recovered in 43 cases (89 6%) . 24 cases of dysmenorrhvea relieved. 21 of 36 patients who hoped for pregnancy became pregnant(58.3%).The pregnant rate of patients with light uterin cavity adhesion was highest, followed by moderate uterin cavity adhesion, and there was no pregnancy in the cases of serious uterin cavity adhesion(? 2=6 826, P =0 033). Conclusions Hysteroscope combined with B-ultrasound to treat uterin cavity adhesion is a preferable method. Pregnancy after operation was closely related to degree of uterin cavity adhesion.

Objective To investigate Hepatitis B Virus (HBV) infection status in placentas of Hepatitis B Surface Antigen (HBsAg) positive pregnant women during middle and late period of pregnancy. Methods One hundred and sixty seven placentas from HBsAg positive pregnant women were collected, including 158 term placentas and 9 aborted second trimester placentas. HBsAg and hepatitis B core antibody (HBcAg) in placental cytotypes were detected by immunohistochemistry. Results Of the 158 term placentas, HBV infection rates in decidual cells (DC), trophoblastic cells (TC), villous mesenchymal cells (VMC) and villous capillary endothelial cells (VCEC) were 66 46% (105/158), 58 23% (92/158), 27 22% (43/158) and 12 66% (20/158), respectively. The HBV infection rates gradually decreased from maternal side to fetal side of placentas. Both HBsAg and HBcAg were mainly located in the cytoplasm of infected cells. The positive cells were mostly in focal distribution. One out of 9 aborted second trimester placentas was infected. Conclusions HBV infection was found in different cells of term placentas. The positive rates for HBsAg and HBcAg in placentas were gradually decreased from maternal side to fetal side. The positive rate was low in aborted second trimester placentas.