Objective: To investigate the effects of morphine,tramadol and lornoxicam on the secretion of inflammatory mediators by rats'peripheral blood mononuclear cells(PBMC) at their analgesic concentrations. Methods: Mononuclear cells were isolated from peripheral blood of male SD rats by the Ficoll-Hypaque method,and treated with Morphine(50 ng/ml),Tramadol(500 ng/ml) or Lornoxicam(300(ng/ml)) respectively with or without stimulation by Lipopolysaccharide(LPS) 2 ?g/ml.After incubation for 24 h,concentrations of TNF-?,IL-6 and IL-10 were determined in the cell culture supernatants by ELISA. Results: Levels of TNF-?,IL-6 and IL-10 were reduced significantly in morphine groups with or without LPS;Tramadol reduced levels of these inflammatory mediators significantly in the groups stimulated with LPS,while it had no effects on the cells cultured in normal conditions;Lornoxicam reduced IL-6 and increased IL-10 levels in either groups. Conclusion: Morphine nonselectively suppressed mediator secretions either under inflammatory or physiological conditions;Tramadol reduced inflammatory mediator secretion in LPS stimulating group,thus could benefit patients with potential sepsis;Lornoxicam significantly reduced proinflammatory cytokine IL-6 secretion and increased anti-inflammatory cytokine IL-10 secretion,and may be a choice for postoperative hyperinflammation.

Objective To explore the effect of mon-suturing peritoneum in perforating purulent infection of incisional wound of appendicitis. Methods907 patients suffered purulent perforating and gangrenous appendicitis were divided randomly into two groups:the non-suturing group of 355 patients and the control group of 552 patients.Of the non-suturing group,peritoneum was not sutured when his/her abdominal cavity was closed.It was just on the contrary to the control group.Then examined whether eoncotic rythrogenic or tender incision occurred,or whether purulent secretion flowed outside from the operative incision.These items below were also been recorded carefully:the average number of days when the temperature was abnormal,the grades of healing of incision and the average hospital stay. ResultsIn the non-suturing group,21(5.9%)patients suffered postoperative infection,the number of days of abnormal temperature was(3.8 ± 1)d on average,and the average hospital stay was(7.5 ± 1)d;in the control group,119(21.6%)patients suffered postoperative infection,the number of days of abnormal temperature was(4.3 ± 1)d on average,and the average hospital stay was(12.9 ± 1)d.First rate healing of incision in the non-suturing group was far higher than that in the control group(P＜0.05). ConclusionNon-suturing peritoneum could help internal drainage peritoneum,which was an efficient way to guard against the postoperative infection.And non-suturing was also of great significance to reduce the average number of days of abnormal temperature and hospital stay.Meanwhile,the probability of ankylenteron and incisional hernia was not increased.

Methods A cluster random sampling method was used to select left-behind students in Grade 3 to 6 in 5 primary schools in the southern rural area of Ningxia Hui Autonomous Region. Their general information were collected, their social anxiety and loneliness were evaluated by the Child Social Anxiety Scale ( SASC ) and Children's Loneliness Scale ( CLS ). The multivariate linear regression model was used to analyze the influencing factors for the social anxiety of left-behind children.

Objective To determine the protein levels of receptor activator of NF ?B ligand (RANKL) in synovial tissues obtained from ankylosing spondylitis (AS) patients, and compare the expression level and distribution of RANKL protein in AS with that in rheumatoid arthritis (RA), osteoarthritis (OA) and normal synovial tissues, in order to define the role of RANKL expressions in the pathogenesis of arthritis and bone destruction in AS. Methods Immunohistochemical analysis was performed using monoclonal antibodies to determine RANKL expression in 13 AS, 16 RA, 17 OA patients and 6 healthy controls. The protein levels of RANKL in labeled synovial tissue sections were quantified by digital image analysis and semiquantitative analysis to compare the expression of RANKL in positve cells among different patient groups and normal subjects. In addition, RANKL expression was correlated with certain inflammatory indices (including ESR, CRP, blood platelet count) and radiological stage of involved joints, respectively. Results Positive staining of RANKL was seen respectively in all 13 AS patients and 16 patients with RA, and the positive expression was distributed predominantly in the synovial lining layer and at synovium cartilage junctions. There was no significant difference between levels of RANKL expression in tissues from patients with AS and in tissues from RA. No positive staining of RANKL was observed in 6 normal subjects and all OA patients. Positive correlation was found between RANKL protein expression and X ray stage of bone destruction of involved joints in the patients with AS and RA ( r =0 73,0 41, P =0 003,0 021 respectively). Conclusions RANKL plays an important role in the pathogenesis of bone destruction in patients with AS, and its elevated expression level may reflect the degree of bone destruction of peripheral joints in AS patients. Since similar patterns of RANKL expression are found in synovial tissues from AS and RA patients, therefore, we conclude that the pathogenesis of bone destruction in AS may be similar to that in RA

ObjectiveTo explore the application value and techniques of vaginal hysterectomy of non-prolapsed uterus lesion in primary hospital. Methods140 cases who suffered from uterine fibroid without adhesion,glandular fibroids,functional bleeding without uterine prolapse and malignant disease were selected and divided into observation group(80 cases with vaginal hysterectomy)and control group(60 cases with routine abdominal hysterectomy).Then the clinical treatment effect was analyzed. ResultsCompared with control group,peri-operative bleeding (150 ±23)ml vs(243 ±41)ml,operation time(65 ± 14.0)min vs(90 ± 27.0)min,body temperature recovery time (3 ±0.5)d vs(4 ±0.8)d,anus exhaust time(14 ± 1.8)h vs(26 ±2.7)h,the length of time(5 ±0.8)d vs(11 ±2.9)d in observation group were all reduced,differences were significant(all P＜0.01)and no case was transferred to opening appendectomy. ConclusionFor these disease including uterine fibroids with no adhesion,glandular fibroid uterine bleeding,the vaginal hysterectomy was safe and feasible,and had the advantage of smaller trauma,less bleeding,shorter hospitalization time,faster recovery etc.It was worthy to be popularized in primary hospital.

Objective To study the skills and safety of breast conserving surgery in radical mastectomy.Methods A total of one hundred and seventy-eight patients with early breast cancer were randomly divided into two groups: 93 cases in the breast conserving surgery group and 85 cases in the radical group,according to the surgical method.The patients in the breast conserving surgery group received stageⅠbreast conserving surgery after modified radical mastectomy for breast cancer,while the patients in the radical group adopted modified radical mastectomy.Then the two groups were compared in terms of postoperative complications,breast appearance evaluation,follow-up on quality of life and tumor recurrence.Results There were not statistically significant differences in postoperative complication rate between the two groups(30.11% vs.23.53%, χ2=0.976,P=0.323);the rate of excellent breast appearance in the conserving surgery group was 86.02%,significantly higher than that of the radical group(38.82%,χ2=42.675,P<0.001);the scores of physiological status,psychologic status,social function and environmental circumstance in the conserving surgery group were significantly higher than those of the radical group,the differences were statistically significant(P<0.05);the difference on tumor recurrence rate of the two groups was not statistically significant(χ2=0.009,P>0.05).Conclusion After radical mastectomy,stageⅠbreast conserving surgery could help patients maintain their breast appearance and improve their life quality,without any increase in the rate of postoperative complications and long-term local morbidity.It is a safe and reliable surgical method.

Abdominal Neoplasms ; diagnosis ; secondary ; Adult ; Animals ; Female ; Humans ; Liver Diseases, Parasitic ; diagnosis ; Liver Neoplasms ; diagnosis ; parasitology ; pathology ; Neoplasm Metastasis ; Paragonimiasis ; diagnosis

Injury to anterior cruciate ligament (ACL) is the most common lesions of the knee joint. Reconstruction of ACL has been the primary treatment for the instability of the knee. Compared with the traditional reconstruction with patellar tendon, the hamstring tendon has many advantages, such as less damage to the donor site and less incidence of antero-patellar or kneeling pain. This paper highlights the basic and clinical research on ACL reconstruction.

A spinal cord injury at a level above T1 often results in a partial or complete loss of the hand function. Transcutaneous functional electrical stimulation is a method to stimulate the handicapped limb muscle by low frequency impulsive current to generate muscle contraction. The immediate effect can replace or correct the lost function to promote function reconstruction through adjusting high-level nerve center and improve daily activity. Functional electrical stimulation provides a efficient therapy for the rehabilitation of patients with spinal injury, and brings a hope to improve the quality of life of paraplegina patients. However, transcutaneous functional electrical stimulation requires assistance to place the equipment on the patients and technical support. Both patients and their families often have too high expectations from this therapy, and become disappointed since the results are not satisfactory. Therefore, we should continue to carry out studies on the parameters and equipments of functional electrical stimulation, and renovate the stimulator to make it more convenient and efficient.

OBJECTIVE To evaluate the current status that nosocomial infection constitutes one of the main problems threatening the safety of hospitalized patients and to set the idea that a good practice of hand hygiene will definitely improve the situation.METHODS The published papers and related regulations in this specific field were reviewed.RESULTS The most common way responsible for nosocomial infection was the transmission of pathogens by hands of medical staff;the detection rate of pathogens was as high as 80% in nursing staff hands,which included Pseudomonas aeruginosa,Escherichia coli and Staphylococcus aureus by rates of 25.9%,22.2% and 14.8%,respectively.CONCLUSIONS It is of great importance to control nosocomial infection by realizing and using the hand hygiene knowledge,which is basic,simple,and practicable.