[Objective] To investigate and analyze the clinical effect of double-plate method on complex tibia plateau fracture. [Methods] A total of 62 pa-tients that had complex tibia plateau fracture from October 2010 to December 2012 in our hospital, were divided into the experimental group and the control group randomly, 31 patients per group. Patients of the experimental group received double steel plate internal fixation with double incision, and patients of the control group received locking plate internal fixation, and the clinical efficacy of two groups were compared. [Results] The excel ent rate of the experimental group was better than that of the control group(87.1% vs 64.5% χ2=4.309 P=0.038), and the difference of complications rate of two groups had no statistical significance(3.2%vs 6.5% χ2=0.350 P=0.554). [Conclusion] The clinical effect of double-plate method for complex tibia plateau fracture is good, and the complications rate is low, worthy of clinical promotion.

Objective To investigate the effect of batroxobin on the plasma levels of endothelin (ET) and calcitonin gene-related peptide(CGRP) in patients with acute cerebral infarction(ACI). Methods 60 patients with ACI were randomly divided into therapy group(n = 30) and control group(n = 30),500 ml of 5% GS plus 1.0g ramme of Troxerutin was intravenously dripped par day and ticlopidine 250mg/day in the control group. Based on the control group therapy,Batroxobin was intravenously dripped with 10U on the 1st day and 5U on the 3rd and 5th day for seven days. The plasma levels of ET and CGRP were detected pre and post treatment by radioimmunoassay. Results Plasma levels of ET and CGRP in batroxobin therapy group post treatment ( 81.25±27.38 ) ; ( 44. 13±13.30) ng/L were significantly different from those pre treatment(109. 83±38.24) ; (24. 73±13.59) ng/L (all P＜0. 01 ) ,and were also significantly different from those post treatment in the control group(98.07±30. 39) ; (37.02±12. 41 )ng/L (all P＜0. 01 ). Conclusion Batroxobin can accommodate the equilibration between ET and CGRP, and protect brain cell and blood vessel endothelial cell.

Objective To explore the influence and potential protective effects of non-specific panMetrix Metalloproteinases(MMPs) inhibitor Doxycycline on hyperoxic lung injury and lung development in neonatal rats.Methods Neonatal SD rats were divided into 4 groups randomly within 12 hours after birth:air with normal saline(AN),air with doxycycline(AD),hyperoxia with normal saline(ON),and hyperoxia with doxycycline group(OD).Hyperoxia groups(ON and OD) were exposed to ≥90% O2.Doxycycline 20 mg/kg or equal volume of 0.9% saline(Doxycycline concentration 2 mg/ml) was administered by gastric gavage,twice daily from day 1 to experimental day,but it didn't exceed 14 days.Stereological study was carried out at day 1,3,7,14 and 21 after birth.Results Enlarged mean alveolar area was noted both in hyperoxia and Doxycycline groups.OD group had larger star volume than ON group on day 14.Except ON group,all other groups had increased alveolar septum and the condition was worsened by hyperoxia with Doxycycline.The percentage of collagen in lung parenchymal tissue in ON group increased persistently.This increasing trend was stopped in OD group on day 14,and collagen percentage had no significantly difference between OD and AN groups on day 14 and 21.Conclusion Both hyperoxia and Doxycycline influence neonatal lung development,percentage of coUgen in lung parenchymai can be reduced by Doxycycline in hyperoxic lung injury.

A representative nitrogen doped titanium dioxide sample (denoted as N-doped TiO_2) was prepared by treating nanotubular titanic acid (H_2Ti_2O_5·H_2O,NTA in short) in flowing NH_3 at an elevated temperature of 500℃ for 4 h.The sub-band structure within Eg of N-NTA-500 was determined by means of photoluminescence (PL) spectrometry and ultraviolet-visible light-near infrared (UV-Vis-NIR)diffusion reflectance spectrometry (DRS).Moreover,the photocatalytic activity of N-NTA-500 was evaluated by performing the redox reaction of propylene under the irradiation of alternate on-and-off visible light,using commercially available P-25 titania as a comparison.It was found that a large amount of single-electron-trapped oxygen vacancies (denoted as Vo~·) formed a sub-band within the Eg of N-NTA-500,contributing to extend the optical absorption of N-doped TiO_2 into visible light region.The sub-band width,the bottom energy above the top edge of valence band,and the top energy below the bottom edge of conduction band were determined to be 0.24 eV,1.79 eV,and 0.99 eV,respectively.Results and discussion:Each of the four PL spectra at an excitation wavelength of 360-390 nm can be deconvoluted into two bands.The peak positions of the two deconvoluted PL bands for each of the four PL spectra at λ_(ex)=360-390 nm are unchanged and located at 465 nm and 570 nm,respectively,implying that they are not originated from the back band-to-band transition but the e~--h~+recombination on two surface states.The band at 465 nm represents the emission on shallow surface state (2.66 eV above valence band edge),while that band at 570 nm represents the emission on deep surface state (2.17 eV above valence band edge).Moreover,the PL peak intensity decreases with increasing excitation energy,indicating that the photogenerated energy-enriched electrons are firstly relaxed to the bottom of the conduction band and then transferred to the surface state,and the larger the value of E_(ex),the more the energy loss.Therefore,it can be concluded that the excitation at λ_(ex)≤400 nm for N-NTA-500 is dominated by band-to-band transition,while E_g of the N-doped TiO_2 is identical to that of TiO_2 (anatase),I.e.,3.1 eV at 400 nm.The emission mechanism with respect to band-to-band transition of N-NTA-500 in an excitation wavelength range of 360-390 nm is depicted.

Objective To investigate the inhibitory effects of Snail—— a zinc finger transcription factor, anti-sense plasmid on the invasion of ovary carcinoma cell lines in vitro. Methods Four human ovarian carcinoma cell lines ES-2,OVCAR3,HO8910,HO8910PM were analyzed for the expression of Snail and E-cadherin mRNA by RT-PCR.Then anti-Snail plasmid transfection was introduced into HO8910 cells using lipofectin 2000 reagent to investigate the inverse correlation between E-cadherin and Snail expression, and the inhibitory effects of anti-sense Snail were also detected by Transwell motility assay and Matrigel invasion assay. Results The results demonstrated the presence of Snail mRNA in ES-2,HO8910,HO8910PM detected by RT-PCR. By contrast, E-cadherin mRNA was only detected in OVCAR3 by RT-PCR.The inverse relationships were further observed by transient transfection of anti-sense Snail into HO8910 cells, which showed the down-regulated expression of Snail and the re-expression of E-cadherin. The Snail mRNA levels were 0.897?0.005,0.865?0.010,0.338?0.014 after 0,24,48 h of transfection and that of E-cadherin were 0,0.130?0.001,0.217?0.005 respectively, the difference was significant between different time points (P

Complement-induced polymorphonuclearcytes (PMN) aggregate in microvasculature of vital organs, produce microemboli, and block microcirculation blood flow. Activated PMN release oxygen free radicals, prostaglandins and proteases, in turn, damage endothelial and parenchyma cells. This is an important factor in pathogenesis of multiple system organ failure. We have found that Sinomeuine and Sodium Ferulate prevented PMN aggregation induced by activated complement. Adding the drugs into PMN suspension treated with cytochalasin B, the zymosan activated plasma, PMN aggregation was prevented. The changes of light transmission were smaller than those of control without drugs recorded with platelet aggregometer. The 50% of PMN aggregation inhibition with Sinomeuine was 0.76mg/ml (1.gmmol/L), and 0.82mg/ml (3.8mmol/L) with Sodium Ferulate.

Objective To explore the primary treatment of type 2 diabetes, clinical effect of rosiglitazone combined with metformin treatment.Methods In 40 patients with type 2 diabetes mellitus patients implementation of routine treatment for metformin, and 40 patients in the control group based on the use of rosiglitazone intervention treatment, two groups of patients in our hospital from January 2015 to December 2016 were compared between the two groups.Results Treatment efficiency, obvious effective rate of observation group were higher in the observation group was 95%, while only 82.5% of the control group,comparison between groups showed significant differences(P<0.05);the two groups of patients with fasting blood glucose levels, 2 hour postprandial blood glucose level There were no significant difference before treatment, after treatment group observation group significantly improved, comparison between groups showed significant differences(P<0.05).Conclusion Rosiglitazone Combined with metformin in newly diagnosed type 2 diabetes clinical observation, which can help to improve the clinical symptoms of patients, reduce blood glucose, compared with pure medication.To improve the treatment effect, it is worthy of reference.

The connotation development of industry and education is the road to advance the development of connotation and the quality of higher vocational and technical education.Based on this and bringing into play the advantages of running a vocational school and combined with cultivating the applied personnel needed by enterprises and professions,the college perfects the personnel cultivation model.carries out the "225" education model,accelerate the development of the teacher,promote its service capacity to enhance constantly the quality of higher vocational and technical education.

This paper gave a dynamic analysis on the health resource allocation and utilization of Chongqing since it became a municipality,found out the extrusive problems existing in the health resource allocation and put forward some corresponding proposals,hoping to benefit the health administration on constituting regional health development planning.