BACKGROUND:Present treatments for chronic skin wounds have certain limitations, and adult stem cels play a potentialpart in cutaneous repair and regeneration.
OBJECTIVE:To review effects of stem cels in skin regeneration and wound healing.
METHODS:The first author retrieved CNKI and Medline databases by computer for relevant articles published from 2000 to 2010. Thekeywords were “epidermal stem cels, hair folicle stem cels, stem cels, transplantation, dermal stem cels” in Chinese and in English, respectively. Then totaly 489 papers were obtained after initial survey, and according to the inclusion criteria, 30articles were selected for review.
RESULTS AND CONCLUSION:Epidermal stem cels and other adult stem celshave beenapplied to treat wounds and other skin diseases. Epidermal stem celsarethe crucialcelsource of skin development, repairandremodeling.Epidermal stem celsarealwaysina resting statein vivo.Unless,skin injure or culturein vitro, celdivision and proliferation wilbe significantly fastened. The stability of the epidermis mainly dependson the asymmetric divisionof a subpopulation, in which two daughter celsare produced, including one with characteristics of stem cels, and the other differentiated into transient amplifying cels that wil be differentiated intopost mitotic celsafter a series of cel divisions (3-5 times). Afterwards, those post mitotic celsaredeveloped into terminal differentiation cels onthe basal layer, finaly detachfromthe epidermisasdander. In addition, it is unclear whether epidermal factors are related to apoptosis, migration and differentiation in the process of wound repairandeven under physiological conditions. Therefore, application ofstem celsinwound healing requiresa further discussion.

Child ; Fibroma ; Humans ; Male ; Nasal Cavity ; pathology ; Nose Neoplasms

4-Chloromercuribenzenesulfonate ; pharmacology ; Adult ; Case-Control Studies ; Female ; Graves Disease ; metabolism ; physiopathology ; Humans ; Hydrogen Peroxide ; metabolism ; Iodine ; metabolism ; Male ; NADPH-Ferrihemoprotein Reductase ; metabolism ; Thyroid Function Tests

Acetic Acid ; analysis ; Chromatography, Ion Exchange ; Workplace

OBJECTIVE:To observe the clinical efficacy and safety of Shenmai injection combined with Trimetazidinee tablet in the treatment of hypothyroid heart disease. METHODS:64 patients with hypothyroid heart disease were randomly divided into control group and observation group. Patients in 2 groups were conventional treatment. Based on the treatment,control group was treated with Trimetazidine tablets 20 mg,orally,3 times a day;based on the treatment in control group,observation group was ad-ditionally treated with Shenmai injection 40 ml by intravenous infusion,once a day. The treatment course was 2 months. The clinic data was observed,including clinical efficacy,and T3,T4,TSH,LVEF,LVEDD and LVESD before and after treatment,pericar-dial effusion depth and incidence of adverse reactions. RESULTS:The total effective rate in observation group was significantly higher than control group(P<0.05). After treatment,T3 and T4 in 2 groups were significantly higher than before,TSH and LVESD were significantly lower than before(P<0.05),however,the difference was not statistically significant between 2 groups (P>0.05);LVEF in 2 groups was significantly higher than before,observation group was higher than control group,LVEDD and pericardial effusion depth were significantly lower than before,observation group was lower than control group(P<0.05). There were no obrious adverse reactions during treatment. CONCLUSIONS:Based on the conventional treatment,Shenmai injection com-bined with trimetazidinee has better efficacy than only trimetazidinee in the treatment of hypothyroid heart disease,with similar safety.

Objective To establish the method for detemining the content of ephedrine hydrochloride in Zhike Pingchuan Tangjiang by HPLC. Methods Diamonsil ODS1 C_(18) Column was used with acetonitrile -0.1% phosphoric solution (with 0.1% triehylamine) (3 : 97) as the mobile phase, the detection wavelength as 205 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.12～ 0.96 ?g for ephedrine hydrochloride and linear equation was Y= 109759X+3792.8, r=0.9998. The average recovery was 98.4% and RSD was 0.87% (n =5). Conclusion This method was simple, accurate and proper, with good reproducibility. It can be used for quantitative analysis of ephedrine hydrochloride in Zhike Pingchuan Tangjiang.

Adult ; Aged ; Carcinoma, Squamous Cell ; surgery ; Esophagus ; surgery ; Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; Male ; Middle Aged ; Phonation ; Postoperative Period ; Reconstructive Surgical Procedures ; methods ; Speech, Alaryngeal ; Trachea ; surgery

Objective To amplify the 16S RNA fragments of 7 clinically isolated strains of Brucella spp. by PCR-RFLP technique, so as to provide experimental basis for the studies on diagnostics, genetics and epidemiology of Brucella spp. Methods According to the gene sequence of ATCC 25840 standard strain in GenBank, special primers for the 16S RNA conservative area in the Brucella spp. were designed. DNA extraction and PCR amplification of the 16S RNA fragments were performed with the 7 isolated strains. PCR products were then sequenced and RFLP analysis was conducted with appropriate restricted enzymes to study the homology and the mutation sites in those strains. Meanwhile, the clinical data of infected patients were retrospectively analyzed to evaluate the relationship between the clinical features and genotypes of Brucella infection. Results The amplified target fragments were about 1500bp in length and consistent with what was expected. The sequencing and homology analysis showed a 98.88% homology and 11 mutation sites among the 7 isolated strains. Four genotypes were identified by RFLP. Retrospective analysis of the clinical data indicated that no obvious relationship existed between the genotypes and the clinical features. Conclusions Amplifying 16S RNA fragments by PCR technique is a feasible method to make an early diagnosis of Brucella infection. The 7 clinically isolated strains are different in genotypes and 16S RNA fragment is a highly conservative fragment in bacterial genome with some mutations. The research provides evidence for the genetics and epidemiology of brucellosis.

Objective To observe the abnormality on behavioral ability of transgenic mice for HRX-EEN fusion gene.Methods Transgenic mice for HRX-EEN fusion gene(transgenic group,n=12)and C57/BL mice(control group,n=12)were tested in hidden platform training(day 1 to day 4)and probe trial testing(day 5)in Morris water maze in which ability of spatial learning and retention was assessed.Results In hidden platform training,the latencies of transgenic group were longer than those in control group,and significant differences were observed between the two groups for day 2,3 and 4(P

Adult ; Genotype ; Humans ; Male ; Middle Aged ; Peptidyl-Dipeptidase A ; blood ; genetics ; Pneumoconiosis ; blood ; genetics ; Polymorphism, Single Nucleotide