Bicuspid aortic valve(BAV) is one of the most common congenital cardiovascular malformations,affecting 1% to 2% of the population.BAV may be sporadic or familial.BAV is likely a multiple genetic disorder based on the study of heritability and identification of pathogenic genes and potential candidate genes.With increasing studies of related genes of BAV,early intervention and gene therapy of BAV and associated complications may be likely in the near future.

Objective To assess the effects of tibolone treatment on the hemodynamic changes of the ophthalmic artery(OA) and internal carotid artery(ICA) as sonographic markers of atherosclerosis in menopausal women.Methods A total of 30 menopausal women were treated with 2.5mg of tibolone(Livial) daily as treatment group.At the same time,30 women with natural menopause who were not given any treatment served as control group.Noninvasive measurements of the resistance index(RI) and peak systolic blood flow velocity of ICA and OA were made with ultrasound before the treatment and at 3rd,6th,9th and 12th month of treatment,respectively.Results RI of ICA and OA decreased significantly after tibolone treatment(P

Objective To investigate the expression of cyclin D3 and proliferating cell nuclear antigen (PCNA) and their clinical significance in mal ignant melanoma. Methods The expression of cyclin D3 and PCNA was measured by streptavidin-peroxidase complex immunohistochemical technique in 57 cases of pri mary cutaneous malignant melanoma (CMM), 37 cases of metastatic melanoma and 20 cases of benign nevi. Results The positive expression rate of cyclin D3 in CM M and metastatic melanoma were 35.1% and 59.5% respectively, while the high expr ession rate of PCNA were 57.9% and 78.6% respectively. Compared with that in ben ign nevi, the expression of cyclin D3 and PCNA was significantly increased. The expression of cyclin D3 and PCNA in CMM was positively related to Clark′s grade and the metastasis to lymph nodes. The 3-year survival rate in patients with ne gative expression of cyclin D3 was significantly higher than that with positive expression in superficial melanomas. The 3-year survival rate of patients with l ow expression of PCNA was significantly higher than that with high expression in CMM. The expression of cyclin D3 was positively correlated with the high expres sion of PCNA in superficial and metastatic melanomas. Conclusions The expressi on of cyclin D3 and PCNA may be involved in the carcinogenesis process and progr ession of CMM and have important implications in the selection of therapeutic re gimens and prognostic assessment. The expression level of cyclin D3 may be regar ded as a prognostic factor for superficial melanoma.

Objective To investigate the infection characteristics of human cytomegalovirus(HCMV)in infants of Huai′an area . Methods Clinical data of 72 infants with HCMV infection ,diagnosed from Nov .2013 to Feb .2015 ,were retrospectively analyzed , including HCMV DNA level of urine samples ,detected by using fluorescent quantitative‐polymerase chain reaction (FQ‐PCR) ,and complications and clinical characteristics .Results The positive rate of HCMV DNA was 90 .3% (65/72) .Among all of the infected infants ,60 cases (83 .3% ) were with pneumonia ,34 cases(47 .2% ) were with hepatitis ,14 cases(19 .4% ) were with hematological diseases ,3 cases(4 .2% ) were with neurological diseases ,and 28 cases(38 .9% ) were with two or more than two types of complica‐tions .Conclusion FQ‐PCR might be useful for the detection of HCM V infection .Clinical manifestation of pneumonia ,hepatitis ,he‐matological diseases ,and so on might be common in HCMV infected infants .FQ‐PCR ,used to detect HCMV DNA ,could be helpful for the early diagnosis and treatment of HCM V infection .

Congenital vahular aortic stenosis (AS),which refers to a congenital malformation of the aortic valve,is the most common causes of left ventricular outflow tract obstruction in chiidren.In 1983,percutaneous balloon aortic valvuloplasty (PBAV) was firstly described as the treatment for congenital AS in children.Over the past 30 years advancement in technique and equipment had radically improved the safety and outcome of balloon valvuloplasty of aortic valve.Nowadays the technique is widely regarded as the therapy of first choice for children with congenital AS.However,it is essential to make decisions about treatment and define which approach has the best chance of success for any given patient before the initial intervention,especially for the neonate with critical AS.PBAV is usually effective for acutely relieving left ventricular outflow obstruction and has excellent short term results in most childen with congenital AS.The mid-and long-trem outcome after a successful balloon dilation of congenital AS in childhood is also good,but late restenosis and valve regurgitation eventually necessitate reintervention in the majority of children,which included aortic valve replacement.PBAV was found to have a high incidence of complications and remains one of the most challenging procedures in the inttcventional therapy of congenital heart disease.Operators should not only acquire the necessary manual skills,but also have the ability to make sound clinical judgment and deal with critical events.Owing to its palliative nature of balloon dilation for congenital AS,the follow-up care of patients after procedure is quite important.

Heart failure is one common critical cardiovascular disease in children.Angiotensin convening enzyme inhibitors plays an important role in the treatment of pediatric heart failure.In this paper,the pharmacological mechanism,pharmacokinetics,clinical studis and application of angiotensin converting enzyme inhibitors in the treatment of pediatric heart failure were concerned.

Child ; Humans ; Tachycardia ; physiopathology ; therapy

Premature ventricular complex (PVC)-induced cardiomyopathy is due to frequent PVCs and it can be potentially reversible in selected patients resolves after catheter ablation.PVCs burden is importantly associated with impaired left ventricular(LV) function.Necessary evaluation is important for PVCs patients.For patients with high PVC burdens above 15％-25％ of the total cardiac beats in 24 hours,medical treatment should be used to reduce PVC burden and to improve LV function.Catheter ablation of PVCs is recommended for selected patients with high PVC burdens associated with a decline in LV systolic function.

Objective To observe the effects of hormone replacement therapy (HRT) on endothelial function in menopausal women. Methods A total of 30 menopausal women were treated with 2.5 mg of Tibolone (Livial) daily. At the same time, 30 women with natural menopause without any treatment served as the control group. Endothelium-dependent (EDD), endothelium-independent (NID) vasodilatation function, and estradiol (E2) were examined by the non-invasive high-resolution ultrasonography before the treatment and at 12th, 24th, 36th and 48th week of treatment, respectively. Results After hormone treatment, E2 increased significantly and EDD was improved significantly (P<0.05), and E2 was positively related with EDD (r=0.8092, P<0.001). No change of EDD was observed in the control group whereas a significant increase was observed in the treatment group. Conclusion Endothelium-dependent vasodilatation dysfunction is prominent in menopausal women. Tibolone can help improve the condition.

Objective To explore the practicle value of video-electroencephalogram(VEEG)in diagnosis of epilepsy in childhood.Methods One hundred and sixty-six children diagnosed as epilepsy group and 49 children suspected to be epilepsy(suspicious epilepsy group) were monitored with VEEG instrument(PI VEEG 400-C) for 3-4 hours under the state of awaking and sleeping and with all kinds of evoked tests.Results Among 166 cases of epilepsy group,124(74.5%) patients were confirmed as epilepsy,clinical seizures were observed in 26 cases of suspicious epilepsy group,6(23.1%) cases were identified as non-epileptic attacks;Among 49 cases,13((26.5%)) patients were confired as epilepsy,clinical seizures were observed in 16 cases,13(81.3%) cases were identified as non-epileptic attacks.Seizure types were difined in 99 of 137(72.3%) children with epileptic discharges,and epileptic syndromes were defined in 43((30.6%)) of them.Conclusion VEEG can provide accurate and reliable information in diagnosis and classification of epilepsy.