Contrast-enhanced ultrasound(CEUS)can afford more information of the vascularization of pancreatic tumors.Adenocarcinomas are often hypovascularized as compared with surrounding tissue,while neuroendocrine tumors are hypervascularized lesions.The most common enhancement pattern of masses associated with pancreatitis ale isovascularizatiom CEUS can improve the diagnosis and differential diagnosis ability in pancreatic tumors.The clinical application value of CEUS is significant.

Objective To study the effects of γ-aminobutyric acid ( GABA) and sertraline on the CABAA(α) receptor and GABAB receptor of hippocampus neuron in rats of depression induced by acute stress. Methods The cognitive function of male SD (Sprague-Dawley) rats was screened through Y-maze. Then rats were randomly divided into five groups. Except control group,rats were injected intraperitoneally with double-distilled, GABA,sertraline or GABA + sertraline respectively. They were exposed to the forced-swimming stress test,which was to make acute stress model of depression. The space memory function in the maze was measured. Then the number of GABA-like immunoreactive neurons in hippocampus CA1 ,CA3 ,DG areas of rats were investigated with immunohistochemist. Results Compared with the model group,the immobility time in the forced-swimming test and the latency in the maze could be reduced significantly after pretreatment with GABA and sertraline (P < 0.01). The positive cell populations of CA1 ,CA3 region and DG gate region in the model group were significantly reduced compared with the control group (P<0.01). GABA could only improve the positive cell populations of GABAB receptor in CA3 region (P<0.01). In the sertraline group,the positive cell populations in hippocampus were increased obviously more than it in model group(P<0.01). The positive cell populations of hippocampus in the GABA + sertraline group in CA1 ( (82.83±8.72),(78.08±5.67)),in CA3((92.83±9.35),(76.00±3.97)),in the gate of DG( (35.00 ±1.41) ,(33.33±4.36)) increased significantly to the other groups (P< 0.05 or 0.01). Conclusion Ectogenic GABA could improve the sertraline' s effects of antagonizing acute depression effectively and the space memory function,and its mechanism may be involved in strengthening the expression of GABAA(α) receptor and GABAB receptor in hippocampus.

Objective To investigate the application of ureteral catheter in complicated gynecological laparoscopic surgery for the prevention of ureteral injury . Methods A total of 182 cases of gynecological laparoscopic surgery performed from January 2009 to November 2011 were selected as study group .Bilateral ureteral catheters were inserted under cystoscopy , and then laparoscopic surgery was performed under the instruction of the catheter .The catheter was immediately removed after the surgery .A total of 200 cases of laparoscopic surgery were selected as control group and preoperative ureteral catheterization was not applied in this group .The incidence rate of ureteral injury was compared between the two groups . Results The success rate of bilateral ureteral catheterization in the study group was 98.9%(180/182)and no ureteral injury occurred .In the control group, ureteral injury was found in 2 cases, which were converted to open surgery for ureter repair and double J stent was retained .The 2 cases recovered 2 months after the surgery.Bladder injury was found in 2 cases during the surgery , which received immediate repair assisted by surgeons from the department of urinary surgery and recovered 2 months after postoperative catheterization . No statistical difference was found in complication rate between the two groups ( P=0.125 ) . Conclusion The application of ureteral catheterization in complicated gynecological laparoscopic surgery can reduce the risk of intraoperative ureteral injury .

With acupuncture depth as a perspective, some related issues are discussed in this paper. Firstly, the possible reasons of divergence on needling depth are explained. Secondly, by literature retrieval, the advantages and disadvantages of the clinical efficacy of deep needling and shallow needling are explored, and the essential value of shallow needling is emphasized in promoting acupuncture. Thirdly, due to the placebo acupuncture that led to the controversy of the curative effect of acupuncture, it is crucial to establish a method of modern system of scientific research which should be suitable for the development of acupuncture. Finally, the "cun" in acupuncture is inappropriate to transform into millimeter, and the standardization of acupuncture should focus on the improvement of curative efficacy. From the acupuncture depth, it is clear that there are plenty of problems in acupuncture, which could not only limit the development of acupuncture, but also affect the future of acupuncture.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Needles

Adult ; Humans ; Laryngeal Neoplasms ; Male ; Neurofibroma ; Vocal Cords ; pathology

Chromatography, Ion Exchange ; Hemoglobins, Abnormal ; analysis ; Humans ; Male

Objective To study the metabolic characteristics of temporal lobe injury during the radiotherapy in the elderly with nasopharyngeal carcinoma through 1H- magnetic resonance spectroscopy (MRS). Methods The 15 aged and 23 young cases among 80 cases with nasopharyngeal carcinoma confirmed clinically and receiving radiotherapy for the first time were involved in the same period of July 2006 to May 2009. Conventional MRI scanning and bilateral temporal MRS were performed before and during radiotherapy at radiation dosages of 20 Gy, 40 Gy, and 60 Gy respectively. The spectroscopy morphology and the ratios of the three main metabolites were compared and analyzed. Results The values of N-acetylasparate (NAA)/creatine (Cr) was lower and choline-containing compound (Cho)/Cr ratio was higher in aged group than in young group before radiotherapy (P＞0.05). The spectroscopy morphology stepped down, NAA, Cho, NAA/Cr, Cho/Cr and NAA/Cho decreased to some extent along with the adding of radiation dosages in the course of radiotherapy. At the end of radiotherapy, the decreased amplitude of NAA/Cr value was larger in aged group than in control group (P＜0.01), and the amplitude of Cho/Cr value was lower than other group. Conclusions The neurons of aged patients with nasopharyngeal carcinoma have a weak tolerance to the radiation, brain injury occurs earlier and heavier in aged patients than in young patients. It is necessary to prevent neuron injury early.

Objective To observe the application effect of the Iodophors hygro-gauze on tracheotomy wound dressing. Methods Fifty patients who underwent tracheotomy with Ⅲ incision, were chosen to be divided into the control group and the observation group with 25 in each group. With saline water cleaning incision and surrounding skin, then disinfection with 0.5% iodophors sanitizers tampon. The observation group applied 0.5% Iodophors hygro-gauze on the wound, the control group used vaseline oil yarn. 24 hours after sample inspection, again samples were sent for bacteria analysis, bacterial colonies number, pathogenic bacteria growth and local situation of wound were observed and analyzed. Results The first results upon disinfection showed that bacterial colonies number, pathogenic bacteria growth between the two groups had no difference. After 24 hours sampling showed that the colony count and pathogenic bacteria growth of the observation group was obviously less than the control group. Local wound infection of the observation group was lighter than the control group. Conclusions 0.5% Iodophors hydro-gauze applied for tracheotomy wound dressing can effectively kill various pathogenic microorganism, prevent secondary infection, and also can prevent and treat wound infection.

Objective: To establish an HPLC-MS/MS method for the determination of hypericin in rat plasma. Methods: The sample was analyzed by HPLC-MS/MS after the addition of internal standard and then protein precipitation using acetonitrile. The sepa-ration was carried out on an Ultimate C18 column (150 mm × 2. 1 mm,5. 0 μm). The mobile phase was composed of acetonitrile∶ 5 mmol·L-1 ammoniumacetate (containing 0. 1% formic acid) (90∶10) at a flow rate of 0. 5 ml·min-1 under 35℃. The detection was performed with multiple teactions monitoring ( MRM) using an electrospray ionization ( ESI) . The precursor/product ion transitions were monitored at m/z 503. 2→m/z 405. 1 for hypericin and m/z 355. 0→m/z 41. 9 for the internal standard pioglitazone ( anion mode). Results:The good linearity of hypericin was obtained within the range of 0. 1-13. 2 ng·ml-1. The correlation coefficient was more than 0. 99 and the lower limit of quantification was 0. 1 ng·ml-1. The extraction recovery was within the range of 84. 19%-98. 71%. The precision of intra-and inter-day was below 18. 47%. Conclusion: The method is fast, sensitive and accurate, which provides research basis for the clinical further study of hypericin.

Three-dimensional conformal radiation therapy(3DCRT) is a new physical method which can enhance the therapeutic gain factor(TGF) of radiotherapy. It can escalate the doses of local tumor and decrease the doses of normal tissue. For patients with primary liver tumors, 3DCRT can increase the local control rates, prolong patients survival and decrease the incidence of radiation-induced complications.