Objective To investigate the influence of thrombospondin 1 (TSP-1) on the expression of vascular endothelial growth factor (VEGF) in human renal tubular epithelial cells. Methods Human renal tubular epithelial cell (HKCs) line was cultured in vitro with DMEM/F12 1∶1 medium. HKCs were divided into four groups as follows: normal control group [HKCs were cultured with serum free medium (FSM), C group], TGF-? 1 group (HKCs were treated with FSM containing TGF-? 1, T group), antisense oligonucleotide group [HKCs were treated with FSM contained TGF-? 1 and TSP-1 antisense oligonucleotide (AS), TA group], missense oligonucleotide groups [HKCs were treated with FSM contained TGF-? 1 and TSP-1 missense oligonucleotide (SC), TS group]. Cells were cultured 72 hours in the conditioned media. The expressions of TSP-1 and VEGF were detected with immunohistochemistry. The expressions of TSP-1 mRNA and VEGF mRNA were assayed by RT-PCR. The correlation between the relative expressions of VEGF mRNA and TSP-1 mRNA was analyzed. Results There were significantly increased expressions of VEGF and VEGF mRNA in TA groups (P0.05) compared with T groups. The expressions of VEGF and VEGF mRNA were decreased significantly in T groups and TS groups (P0.05) compared with T groups. The expressions of TSP-1 and TSP-1 mRNA were increased significantly in T groups and TS groups (P

Objective To explore the value of 31P magnetic resonance spectroscopy(31P MRS)for differential diagnosis of bone malignancy and inflammation.Methods Radiography.MRI and 31P MRs were performed on 20 bone malignancy patients,22 bone and soft-tissue inflarnmation patients and 32 healthy voluntecrs.The spectra were analyzed by measuring the areas under the peaks of various metabolites,and by calculating the pH from the Pi shift relative to PCr.The data were analyzed by one-way analysis of vailance (ANOVA).Results Analysis of the 31P MRS data suggested differences among the bone malignancy,the inflammation and the healthy group.The mean of PME/β-ATP ratio(1.24±0.37)in malignancy group increased significantly(P＜0.01).The mean of PDE/β-ATP(2.21.±0.37),Pi/β-ATP(1.46±0.43)in inflammation group was higher than that of the other two groups(P＜0.05),but the ratio of PME/β-ATP (0.19±0.10)wag not increasing.The LEP/T31P(0.10±0.02),PCr/T31P(0.45±0.03)and ATP/T31P (0.45±0.03)ratios in control group were significantly difierent from the two others(P＜0.01).The mean of intracellular pH in malignancy group was 7.45±0.16,higher than that in control group(7.05±0.06),and in inflaruination group(7.20±0.13)(P＜0.01).Conclusions The significant increase of PME and intraeellular pH is of great value in diagnosis of bone malignancy.Combined conventional radiography and MRI.31PMRs would be a simple,no-.invasive and effective diagnostic method.

Objective Method discussion of measuring curvature of cervical endplate based on reverse engineering software.Methods The CT scan images data (From C2 to C7) of 8 normal males were selected and inputted into Mimics 10.0 software for 3D reconstruction and separation (cervical spine is divided into C1-C7 isolated reconstructed 3D images).Another software NX Imageware 13.2 is used for further curvature measuring in lines named left,middle,right,anterior,middle,posterior which are derived from each superior and inferior endplate.In each line,15 different points which can perfectly show morphologic characters of the endplate are selected for statistic comparing between different points in the same endplate,different lines in the same endplate,different endplates in the same individual and the same endplate in different individuals.In addition,the correlation between the concavity and the width and depth of endplate is also analyzed.Results From inferior endplate of C2 to that of C6,except superior endplate of C7,there is significant difference between different points in the same line (P＜0.05),while it is opposite in the compare between lines in the same endplate,different endplates in the same individual and the same endplate (except inferior endplate of C4) in different individuals (P＞0.05).It showed significant correlation between the concavity and the width and depth of endplate.Conclusion Reverse engineering software is feasible to be used in evaluate morphologic characters of normal cervical endplate as a new method for measuring the detail parameters of them,it also explore the laws of the curvatures of cervical endplate initially and both of them will be of great use for cervical interbody cage design.

Based on a comprehensive questionnaire survey and analysis of factors which trans formation of Scientific and Technological Achievements in Medical,some strategies to accelerate transformation were proposed by this paper.

Objective To evaluate the method of measurement of total scatter calibrate factor (Sc,p). Methods To measure the Sc,p at different depths on central axis of 6?MV, 15?MV photon beams through different ways. Results It was found that the measured data of Sc,p changed with the different depths to a range of 1%～7%. Using the direct method, the Sc,p measured depth should be the same as the depth in dose normalization point of the prescription dose. If the Sc,p ( fsz, d) was measured at the other depths, it could be obtained indirectly by the calculation formula. Conclusions The Sc,p in the prescription dose can be obtained either by the direct measure method or the indirect calculation formula. But emphasis should be laid on the proper measure depth.

OBJECTIVECombined the optical principle with automatic control technology and computer real-time image detection technology to develop a non-contact system for noninvasive esophageal varices pressure measurement.

METHODSThe system included the adjustable air pump, laser device, image collection and analysis program. The feasibility and accuracy of the system were verified by in vitro experiments.

RESULTSThe bionic vascular pressure measured by this system had good correlation and repeatability with the actual pressure.

CONCLUSIONSThis system is accurate, feasible and has good application prospects.

Objective To explore the potential connection between sympathetic response, heart rate and blood pressure modulation after caloric irrigation in order to study the role of vestibular stimuli in cardiovascular control. Method Efferent splanchnic nerve firing rates, ECG and blood pressure were recorded simultaneously during caloric stimuli on intact anesthetized(CON) rats(n=5), sinoaortic denervated (SAD)rats(n=5) and bilateral vestibular destroyed (VD) rats(n=5). Result It was found that after caloric stimulation with ice water mean blood pressure CON rats with intact reflex became lower and the mean heart rates became slower, splanchnic sympathetic nerve activities increased for a moment and then dropped significantly. SAD rats had significant stronger splanchnic sympathetic nerve activities VD rats after caloric stimulation, and their blood pressures changed to apposite directions. The coupled respiratory component on splanhnic sympathetic nerve activities were strongly affected by the caloric stimulation. Conclusion It is suggested that semicircular canal stimulation participate at least in the short-term blood pressure control mechanism and the role of central nervous system on respiratory drive may also be involved. Baro-reflex and vestibular afferent may play different role in the control of blood pressure they may work synergically in some physiological control processed.

Objective To investigate the value of brachial-ankle pulse wave velocity (baPWV) in the prediction of hypertension in middle-aged men with prehypertension.Methods A total of 2580 middle-aged (35 to 55 years old ) prehypertensive individuals who underwent health check-up during September 2006 and December 2007 in our hospital were recruited for this prospective cohort study.After a 4-year follow-up,2451subjects entered final analysis.Logistic regression analysis was used to assess the value of baPWV in the prediction of hypertension.Results(1) Two hundred and eight subjects (8.5％ )developed hypertension after 4-year follow-up study.( 2 ) At baseline,no significant differences of family history of hypertension,heart rate and total cholesterol were found between normotensive and hypertensive subjects (P ＞0.05 ).(3) Logistic regression analysis showed that in age-adjusted model (Model1),the odds ratio (OR) and 95％ confidence interval (CI) of baPWV (140 cm/s) was 2.20 (1.78 to 2.62 )( P ＜0.01) ; while in multi-factor adjusted model ( Model 3 ),OR and 95c％c CI of baPWV was I.49 (1.15 to 1.73) (P ＜ 0.01).(4) Subjects were stratified by quartiles of baPWV at baseline.OR and 95％ CI of hypertension in those of highest quartile was higher than those of lowest quartile ( Model1:OR =10.9,95％CI 5.1-22.7,P＜0.01; Model 3:0R=2.6,95％CI1.2 -6.1,P＜0.05).Conclusion baPWV could be an independent predictor of hypertension among prehypertensive populations.

Objective To assess the risk of sympathetic nerve injury in oblique lumbar interbody fusion (OLIF) in different lumbar spine segments based on anatomical study.Methods Twenty-four healthy adult volunteers (12 male and female) were selected and routine lumbar spine scanning was performed with MAG MAGOMOM Verio 3.0 T.The anatomical structures of left lumbar sympathetic trunk,abdominal aorta and left psoas muscle were identified on T2 images of L2,3,L3,4,L4,5 intervertebral space.And the anatomical parameters of the OLIF operation approach and the anatomical parameters of the left sympathetic trunk and adjacent structures were measured.The t-test was used to compare the parameters between the different sexes.The comparison of the data between the different segments was performed by the least significant difference (LSD) single factor analysis of variance.Results From the L2,3 to L4,5 segments,the anatomical parameters of the OLIF operation approach and the anatomical parameters of the left sympathetic trunk and the adjacent structures showed regular changes.The distances between the anterior margin of the left psoas muscle and the abdominal aorta from L2,3 to L4,5 were 13.65±4.10 mm to 9.42 ± 4.00 mm in adult healthy male individuals,and 13.89±3.18 mm to 8.38 ± 3.33 mm in female individuals,showing a significant downward trend.The distances between the left sympathetic trunk and the abdominal aorta from L2,3 to L4,5 were 10.76±3.89 mm to 6.68±3.39 mm in adult healthy male individuals,and 11.52±3.02 mm to 6.12±2.95 mm in female individuals,also showing a significant downward trend.There were significant differences in the operation area of OLIF surgery between different segments.The operation area of OLIF surgery was relatively large in L 2,3 segment,and the risk of sympathetic nerve injury was relatively small.The left lumbar sympathetic trunk in the L3,4 intervertebral space was walking front and inside,and there was a greater risk of injury in the OLIF surgery.Conclusion The left lumbar sympathetic trunk located in or close to OLIF surgery operation field in L2-L5 segments.There was a certain risk of sympathetic nerve injury in OLIF surgery,and the risk of sympathetic nerve injury was different in L2,3,L3,4,L4,5 segments.

We report two rare cases of spinal intraosseous schwannoma (SIS) with sustained myelopathy symptoms and provide an updated review regarding SIS in the literature. A 71-year-old man experienced right lumbocrural pain and gait disturbance accompanied with paresthesia and right leg weakness. Imaging examinations revealed a mass with lesions in L4 vertebral body causing bone destruction and spinal cord compression. Complete resection of the well-demarcated tumor and posterior fusion were performed. A 54-year-old female reported bilateral gait disturbance, paresthesia, and numbness without weakness, and imaging revealed a posterior mass from T9 causing spinal cord compression and bone erosion. The tumor was completely separated from the spinal nerve root. The tumors from both patients were confirmed as schwannomas. Tumor recurrence was not observed at the 2-4 year follow-up. Although rare, SIS should be considered during differential diagnosis and can affect treatment planning. SIS symptoms vary depending on tumor location, and fusion is frequently necessary for spinal reconstruction after complete tumor resection.
Aged ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Gait ; Humans ; Hypesthesia ; Leg ; Middle Aged ; Neurilemmoma* ; Paresthesia ; Recurrence ; Spinal Cord Compression ; Spinal Cord Diseases ; Spinal Nerve Roots