Objective To investigate the distribution and expression of tight junction proteins (including elaudin 1, occludin,ZO-1 and JAM-1) in mucosa of rats with reflux esophagitis (RE), and its underline mechanism in pathogenesis of RE. Methods Two hundred and twenty 8-week-old male Wistar rats were divided into sham operation control group (n=10), acid reflux group (n=70), alkaline reflux group (n=70) and mixed reflux group (n=70). The rats were sacrificed at day 3, 6, 9 and 14 after operation. The successful rate of modeling was assessed by evidence of inflammation in middle and low esophagus. Transmission electron microscopy (TEM) was used to observe the morphological changes of tight junction in esophageal epithelium. The mRNA and protein expressions of tight junction proteins were detected by Western blotting and RT-PCR, respectively. And interleukin (IL)-6 expression was measured by immunohistochemistry. Results At day 14 after the procedure, RE model was established in all executed rats. Successful rate of 100% was achieved. The microscopic observation showed that mucosa was damaged and thickened as the disease progressed. With TEM observation, widened intercellular space was noticed with fewer desmosomes. Elevated expressions of IL-6 and tight junction proteins were found in three model groups compared with control group. Whereas the expression of tight junction proteins in individual cells was gradually decreased with continuing hyperplasia in the basal layer. The mRNA and protein expressions of IL-6 and tight junction proteins were increased gradually as disease progressed. Conclusions The highly expression of tight junction proteins, which involves in the mechanism of RE by playing the role of positive regulation and synergism, may be early molecular event in development of RE. And IL-6 is an inflammatory factor in this process.

Professor LI Guo-heng is one of famous TCM old doctors in our country.He is the main outstanding inheritor of Wei Shi Traumatology.He is skill in internal and external use of Chinese herb,manipulation and body guidance technique.He is good at treating injury congestion syndrome,Jin Chu Cao,Gu Cuo Feng with Wei Shi Traumatology.Combining differentiation syndrome with differentiation diseases,and using Chinese herb and manipulation,which has a good clinical efficacy.

Oligodeoxynucleotide containing unmethylated cytosine phosphate-guanosine motif(CpG ODN) may induce high expression of CD80, CD86, CD83, HLA I and HLAⅡ molecules on dendritic cells(DC) and stimulate DC to produce high level of IL-6, IL-12, TNF-? and IFN-?. CpG ODN is demonstrated in vivo to be a very potent adjuvant for Th1 cells, regulating Th0 cells to develop toward Th1 cells. Its role for DC is characteristics of CpG ODN sequence specificity and species specificity. CpG ODN is, at present, considered as a pathogen associated molecular pattern which binds its specific receptor,Toll-like receptor 9,then functions through TLR/IL-1R signaling pathway. It may represent a new therapeutic drug for broad applications in infectious disease, autoimmune disease, allergy and cancer therapy.

AIM: To study the effect of oligonucleotides containing unmethylated CpG motif (CpG ODN) on mouse bone marrow-derived dendritic cells (BMDCs) in anti-HcaF cytotoxicity. METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. The proliferation of T cells was examined by MTT assay. Cytotoxicity of CTL induced by CpG ODN combining with TAg was detected by MTT assay. RESULTS: CpG ODN combining or not combining with TAg up-regulated the expression of CD80 on BMDC surface and stimulated BMDCs to produce a high level of IL-12. CpG ODN-activated BMDC promoted the proliferation of T cells. CTL induced by CpG ODN in combination with TAg appeared strong specific cytotoxicity on Hca-F cells. CONCLUSION: CpG ODN may effectively induce the functional maturation of mouse BMDC in vitro. CpG ODN in combination with TAg can enhance the anti-HcaF cytotoxicity of CTL. [

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Objectives To introduce a small incision of anterior extraperitoneal approach to the lumbar spine for single lumbar segment artificial disc replacement. Methods Nine patients suffered from single segment herniation of lumbar intervertebral disc underwent operation of artificial disc replacement. The approach of this operation was with a small incision through medial of left sheath of rectums and access to the retroperitoneal region through extraperitoneal appearance. The clinical and surgical data including the length of incision, length of operation, blood lose, effect and complication of operation were analyzed retrospectively in these 9 patients.Results The average length of incision,time of operation and blood lose was 6 7cm, 150 minutes and 335ml, respectively. The clinical evaluation scales (the 5-point Likert scales for pain, function, economic status, and medication usage) showed great improvement after operation of these patients(P=0 008). No serious complication related with the approach was found. Conclusions The a small anterior incision through peritoneum of left rectus abdominis sheath and access to the retroperitoneal region through extraperitoneal, which can expose the region of lumbar segment from L3 to S1 sufficiently, is a safe and mini-lesion approach for single lumbar segment artificial disc replacement.

AIM: To evaluate the effect of staphylococcal enterotoxin B (SEB) or staphylococcal enterotoxin C (SEC) combining with dendritic cells (DC) on T cell functions and in vitro anti-HcaF tumor cytotoxicity of activated T cells. METHODS: S-100 protein expression in DC was detected by immune histochemistry staining. The expressions of I-E~? and CD80 molecules on DC, the expression of CD69 molecule on T cells and the production of IL-2 and TNF-? by T cells were determined with flow cytometry. The proliferation of T cells and its cytotoxicity to HcaF tumor cells were detected by MTT assay. RESULTS: In vitro experiments showed that isolated DC expressed high level of S-100 protein. SEB or SEC-induced DC highly expressed I-E~? and CD80 molecules and that SEB or SEC-induced DC promoted the activation and proliferation of T cells. 100 ?g/L of SEB or SEC was the most effective concentrations to induce T cells to secret IL-2 and TNF-?. The T cells activated by SEB or SEC combined with DC showed significant cytotoxicity to HcaF cells, appearing a stronger role than tumor antigen combined with DC. There was no difference in the role for T lymphocytes between both SEB and SEC. CONCLUSIONS: The results indicate that SEB or SEC combined with DC is an effective way to enhance T cell functions, producing stronger cytotoxicity to HcaF tumor cells than tumor antigen-loaded DC used at present, which offers a forceful evidence for the possibility of superantigen SEB or SEC combining with DC to be applied to clinical tumor immunotherapy.

The individual variability should be considered in precision medicine-prevention and treatment strategies.Medical research using genomics, proteomics, metabolomics, systems analyses, and other modern tools has made big progress.In 2002, the members of the Complex-Trait Consortium proposed to develop a new mouse genetics resource called the Collaborative Cross (CC).The CC is a genetic reference panel of recombinant inbred lines of mice, designed for the dissection of complex traits and gene networks.It will provide a powerful measure for functional studies of biological networks, which will be essential to understand the intricacies of disease processes.

0.05).CONCLUSION:It is feasible to reduce the dose of hormone in weekly docetaxel chemotherapy