Objective To investigete the clinical and endoscopic featrures of gasrtic varices (GV) in our country. Methods To analyze retroepective study the materials of endoscopically diagnosed GV in 85 patients at our hospital from 1990 to 2000. Result Of 281 patients with varices, GV were detected in 85 (30.2% ) under endoscopy. According to Sarin' a category of GV, GOV - I detected in 63(74. 1 GOV- II 19 (22.4%),IGV- I in2(2.4%) and IGV- II 1 (1. 18%),The common cause of GOV was liver cirrhosis and ICV was segmental portal hypertension without liver disease, No correlation has been observed between the detective rate of GV category and liver function. The rate of portal hypertensive gastropathy (PHC) with GV was higher than that with esophageal varices (EV) only( p

0.05). The blood glucose, glycosylated hemoglobin and oxidized low density lipoprotein degrade, insulin resistance were improved in probucol group after treatment, while the adiponectin level was increased(P

AIM: To construct the plasmid vectors containing different regions of human eNOS promoter coupled to a red fluorescent protein reporter gene, which may express in mammalian cells. METHODS: Different regions of human eNOS promoter were subcloned respectively into a red fluorescent protein vector, pDsRed1-1. These recombinant vectors, pDsF1033Red, pDsF494Red and pDsF166Red, were then transfected into NIH3T3 cell lines, followed by the observation under a fluorescent microscope. RESULTS: After identified to be right by double restriction enzyme digestion, PCR and sequencing, the vectors might be effectively expressed in NIH3T3 cells. 95 % of the red fluorescent emitted by a red fluorescent protein dispersed all over the cells, appearing at 48-60 h after transfection, reaching peak at 96-144 h, becoming the strongest in light at 144 h, gradually disappearing after 168 h and remaining little red fluorescent in 21 days. The quantity and intensity in expressions of red fluorescent protein drived by different regions of human eNOS promoter were clearly lower than by a strong promoter, p CMVIE . CONCLUSION: The red fluorescent protein reporter gene vectors containing different regions of human eNOS promoter are successfully constructed and may efficaciously express in mammalian cells, appearing not strong transcriptional activities, which provide practical and feasible tools to study functions of different regions of human eNOS promoter and roles of cis-elements in it. [