In this experiment, the acute hemorragic necrotic pancretitis(AHNP) animal was pretreated by abdominal cavity injection of recombinant human tumor necrosis factor (rhTNF) or by selective decontamination of digestive tract (SDD) with the use of tybrarmycine, amphotericinum Band polymyxine B orally plus a small dose of polymyxine B by hypodermic injection. The results showed that using exogenous TNF to treat AHNP can reduce mortality of AHNP rats. The mechanism may be that exogenous TNF can induce the tolerance of the host organ(s) to endogenous TNF. SDD plus a small dose of polymyxine B by hypodermic injection can suppress G baeteria grouth and reduce the production of endotoxin in the gut ; and polymyxine B can directly antagonize to the endotoxin of blood, reducing blood endotoxin and TNF level. The results suggest that SDD can be used to treat AHNP.

Extracellular signal-regulated kinase3 (ERK3) is distinguished from other ERK family members especially in its molecular biological characteristics including the big intron between exons in its gene structure, the serine189 mono-phosphorylated site and C-terminal extention of its kinase structure. The specially activating phosphorylation site of serine189 indicates that all MEKs, which phosphorylate serine/threonine double phosphorylation sites of MAPKs, are unable to activate ERK3. The C-terminal extension involves both subcellular localization of ERK3 and binding to intact cyclin D3, which can profoundly affect cell cycle regulation. According to update reports, ERK3 signal pathway in the regulation of cell cycle might be as follows: Ras→B-Raf→ERK3kinase→ERK3→decrease of CDK compounds of G1-phase→increase of the inhibiting factor (retinoblastoma protein) of S-phase→blockage of S-phase of cell cycle→cell differentiation entry while cell proliferation arrest. Moreover, the activation of ERK3 signaling pathway is also associated with cell differentiation, embryonic development, insulin secretion and cancer diseases.

Objective To investigate the distribution and expression of tight junction proteins (including elaudin 1, occludin,ZO-1 and JAM-1) in mucosa of rats with reflux esophagitis (RE), and its underline mechanism in pathogenesis of RE. Methods Two hundred and twenty 8-week-old male Wistar rats were divided into sham operation control group (n=10), acid reflux group (n=70), alkaline reflux group (n=70) and mixed reflux group (n=70). The rats were sacrificed at day 3, 6, 9 and 14 after operation. The successful rate of modeling was assessed by evidence of inflammation in middle and low esophagus. Transmission electron microscopy (TEM) was used to observe the morphological changes of tight junction in esophageal epithelium. The mRNA and protein expressions of tight junction proteins were detected by Western blotting and RT-PCR, respectively. And interleukin (IL)-6 expression was measured by immunohistochemistry. Results At day 14 after the procedure, RE model was established in all executed rats. Successful rate of 100% was achieved. The microscopic observation showed that mucosa was damaged and thickened as the disease progressed. With TEM observation, widened intercellular space was noticed with fewer desmosomes. Elevated expressions of IL-6 and tight junction proteins were found in three model groups compared with control group. Whereas the expression of tight junction proteins in individual cells was gradually decreased with continuing hyperplasia in the basal layer. The mRNA and protein expressions of IL-6 and tight junction proteins were increased gradually as disease progressed. Conclusions The highly expression of tight junction proteins, which involves in the mechanism of RE by playing the role of positive regulation and synergism, may be early molecular event in development of RE. And IL-6 is an inflammatory factor in this process.

Oligodeoxynucleotide containing unmethylated cytosine phosphate-guanosine motif(CpG ODN) may induce high expression of CD80, CD86, CD83, HLA I and HLAⅡ molecules on dendritic cells(DC) and stimulate DC to produce high level of IL-6, IL-12, TNF-? and IFN-?. CpG ODN is demonstrated in vivo to be a very potent adjuvant for Th1 cells, regulating Th0 cells to develop toward Th1 cells. Its role for DC is characteristics of CpG ODN sequence specificity and species specificity. CpG ODN is, at present, considered as a pathogen associated molecular pattern which binds its specific receptor,Toll-like receptor 9,then functions through TLR/IL-1R signaling pathway. It may represent a new therapeutic drug for broad applications in infectious disease, autoimmune disease, allergy and cancer therapy.

OBJECTIVE:To optimize the precipitation process of the extract of Folium Isatidis using chitosan and ZTC1+ 1-Ⅱ natural clarify agents.METHODS:Using the remaining rates of polysaccharide and indirubin and the defecation level as indexes,the optimum process of removing impurity was investigated with single-factor test.RESULTS:The optimum process conditions were as follows:chitosan:precipitating the extract solution which was condensed to 1∶10(herb:the herb solution) with the concentration of chitosan clarify agent at 8% at a heating temperature of 50 ℃;ZTC1+1-Ⅱ:precipitating the extract solution which was condensed to 1∶10(herb:the herb solution) with the concentration of chitosan clarify agent at 5% and the heating temperature at 70 ℃,and the adding sequence of ZTC 1+1-Ⅱ natural clarify agents was clarifier B before clarifier A.CONCLUSION:Chitosan and ZTC1+1-Ⅱ natural clarify agents can be used to effectively remove the impunity of extract of Folium Isatidis.

Objective To investigete the clinical and endoscopic featrures of gasrtic varices (GV) in our country. Methods To analyze retroepective study the materials of endoscopically diagnosed GV in 85 patients at our hospital from 1990 to 2000. Result Of 281 patients with varices, GV were detected in 85 (30.2% ) under endoscopy. According to Sarin' a category of GV, GOV - I detected in 63(74. 1 GOV- II 19 (22.4%),IGV- I in2(2.4%) and IGV- II 1 (1. 18%),The common cause of GOV was liver cirrhosis and ICV was segmental portal hypertension without liver disease, No correlation has been observed between the detective rate of GV category and liver function. The rate of portal hypertensive gastropathy (PHC) with GV was higher than that with esophageal varices (EV) only( p

Professor LI Guo-heng is one of famous TCM old doctors in our country.He is the main outstanding inheritor of Wei Shi Traumatology.He is skill in internal and external use of Chinese herb,manipulation and body guidance technique.He is good at treating injury congestion syndrome,Jin Chu Cao,Gu Cuo Feng with Wei Shi Traumatology.Combining differentiation syndrome with differentiation diseases,and using Chinese herb and manipulation,which has a good clinical efficacy.

Objective To assess the clinical significance of the expression of p53, p21 protein in breast cancer (BC). Method The expression levels of p53, p21 proteins were examined by immunohistochemical (IHC) SP techniques in 69 BC specimen and 20 paracancinoma breast tissue. Result p53 and p21 proteins were failed to be observed in the paracancer tissue. p53 protein was found in 47.8% of BC, while p21 protein in 43.5% of BC. Along with the declined of BC cell differentiation degree, p53 expression increased significantly and p21 expression declined obviously. Expression of p21 was lower in patients with lymph node metastasis than that in patients without lymph node metastasis (P

Objective To investigate and evaluate the preparation of quality control serum of autoantibodies threshold . Methods Donors′ serum blood were placed in 56 ℃ for 30 min to inactivate ,followed by centrifuged to remove the precipitates ,and then multi-batch quality control material in detection kit were detected in dilution ratios of 1∶2 ,1∶4 ,1∶6 .Results Anti-RNP/Sm antibody(+ + + ):after 1∶2 dilution ,the coloration degree was + + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was ++ .Anti-SS-A antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + + ;after 1∶4 ,1∶6 dilution ,the colora-tion degree was + to + + .Anti-RO-52 antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + to + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was + .Anti-nucleosome antibodies :positive were found in dilution of 1∶1 ,1∶2;after 1∶4 ,1∶6 dilution ,the coloration was negative .Conclusion Preparation of quality control serum of autoantibodies is convenient , reliable ,and can meet the needs of clinical detection of internal quality control .

0.05),but the rate of GG genotype in cerebral infarction group was significantly higher than that in normal control group(P