OBJECTIVE:To give some information for the strategy of joint technical innovation in our country.METHODS:To analyse the real situation of joint technical innovation in China and to summarize the experiences of international pharmaceutical corporations.RESULTS & CONCLUSION:Joint technical innovation is the general trend of development,we should take measures according to the existing problems in Chinese pharmaceutical industry.

Objective:To investigate the application of Balanced Scorecard (BSC) in the performance evaluation system of government procurement in medical colleges and universities based on the analysis of the current government procurement process.Methods: According to the theoretical and methodological analysis of BSC, the performance evaluation is divided into four major aspects of assessment indicators, learning and growth, business process, customer and finance, which breaks the traditional performance evaluation way of only focusing on the of financial indicators. Delphi method has been involved to determine the weights.Results: Based on the theory of BSC, the specific evaluation index and weight of the government procurement performance evaluation system in medical colleges and universities are put forward and proved by practice.Conclusion: A reasonable evaluation of the performance system established according to BSC will help to improve the beneficial results, efficiency and economy output of the procurement funds, and the management of government procurement in medical colleges.

This study was aimed to carry out pharmacological research on the hemostatic activity and mechanism of Yi medicine Ma-Bu (Paris polyphylla Smith var stenophylla Franch.). One kind of C27 steroidal saponin from P. polyphylla Smith var stenophylla Franch. was isolated and identified as Paris saponin H (PSH). The effect of PSH on the index of bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB) of mice were measured. The results showed that PSH have significant hemostatic activity by shortening BT. The effect of PSH on shortening PT and APTT of mouse was not significant. However, the FIB was enhanced significantly after treatment with PSH. It was concluded that PSH had no significant promoting effect on the extrinsic coagulation pathway (ECP) or the intrinsic coagulation pathway (ICP). The enhancement of FIB may be a pathway for the effect of hemostatic activity by PSH.

Objective To investigate and analyze the clinical efficacy of psychological intervention in the treatment of uterine atony postpartum hemorrhage and its influence on the quality of life. Methods 100 cases of uterine atony postpartum hemorrhage were selected in our hospital from March 2015 to September 2016.The subjects were randomly divided into the control group and the experimental group, 50 cases in each group. The patients in the control group were treated with oxytocin, and the experimental group was treated with Xinmu Pei combined with psychological intervention.The treatment effect and quality of life total score were compared between two groups. Results After the corresponding treatment, the effective rate of the experimental group was 92.0％, which was significantly higher than that of the control group(72.0％), the difference was statistically significant (P<0.05). The total score of quality of life in the experimental group was (75.2±11.2) points, significantly higher than that of the control group (64.2±9.3), the difference was statistically significant (P<0.05). Conclusion The clinical effect of psychological intervention of hemabate in treatment of postpartum hemorrhage maternal, can improve the therapeutic effect to a great extent, improve the quality of life of patients, with further clinical promotion and application significance.

Objective:To evaluate the clinical effect of Chinese medical chiropractic in treating children with cough variant asthma syndrome (CVA) of Lung-Spleen Qi Deficiency.Methods:From October 2017 to October 2018, 60 CVA children with syndrome of lung-spleen qi deficiency were enrolled from pediatric outpatient department of Maoming Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine, and they were randomly divided into the control group and observation group by random number table method, with 30 in each group. The control group was given montelukast sodium granules orally on the basis of conventional treatment, and the observation group was given chiropractic treatment on the basis of the control group. Both groups were treated for 6 weeks and followed up for 2 months. The TCM syndrome scores were performed before and after treatment, and the changes of serum IgA, IgG, IgM and IgE were detected by ELISA. The recurrence was observed and recorded during the follow-up period, and the clinical effect rate was evaluated.Results:The total effect rates of the observation group and the control group were 86.7% (26/30) and 56.7% (17/30), respectively, with significant differences between the two groups ( χ2=6.648, P=0.008). After treatment, the scores of cough, sputum volume, oppression in chest, tongue coating, concomitant symptoms and total scores in the observation group were significantly lower than those in the control group ( t values were 2.639, 2.628, 2.633, 2.627, 2.725, 2.642, respectively, all Ps<0.05). After treatment, the IgA [(2.31 ± 1.20) g/L vs. (1.55 ± 0.72) g/L, t=2.635], IgG [(11.16 ± 3.39) g/L vs. (8.18 ± 3.27) g/L, t=2.751], IgM [(1.72 ± 0.34) g/L vs. (1.27 ± 0.36) g/L, t=2.660] in the observation group were significantly higher than those in the control group ( P<0.05), while the IgE [(51.32 ± 32.62) g/L vs. (74.58 ± 40.77) g/L, t=2.619] in the observation group were significantly lower than those in the control group ( P<0.05). During the follow-up, the recurrence rate was 19.2% (5/26) in the observation group and 70.6% (12/17) in the control group, and the difference between the two groups was statistically significant ( χ2=4.675, P=0.029). Conclusion:Chinese medicine chiropractic can improve the clinical symptoms and serum immune factor levels of children with cough variant asthma syndrome of lung and spleen qi deficiency, reduce the recurrence rate, and improve the clinical efficacy.

Objective To investigate the effects of epinephrine in sepsis-associated lung injury in rats. Methods Thirty SD rats were randomly divided into three groups(n =10 per group):control group received intravenous 0. 9% saline 2. 4 ml/( kg·h ); LPS group received intravenous LPS ( 6 mg/kg ); epi-nephrine treatment group received an infusion of epinephrine 0. 6μg/( kg·min) after LPS intravenous injec-tion . Blood samples were taken at 2 h and 6 h after LPS injection and the levels of serum tumor necrosis factor ( TNF)-α,interleukin( IL)-6 and IL-10 were detected. The rats were sacrificed at 6 h. The lung tissues and bronchoalveolar lavage fluid( BALF) were collected. Pathological changes of the lung tissues were observed under light microscope. Water content of lung,expression of TNF-α,IL-6 and IL-10 in BALF and in serum were detected. Results (1) The water content of lung in LPS group significantly increased compared with that in control group(85. 24% ± 5. 87% vs. 70. 19% ± 5. 87%) and epinephrine group(78. 00% ± 6. 41%) (P<0. 05). (2)Pathological examination showed that LPS could cause pulmonary capillary hyperemia,ede-ma,inflammatory cells infiltration. Atelectasis and alveolar edema were found in small number of lung tissue. Compared with LPS group, epinephrine ameliorated the lung pathological injury. ( 3 ) Compared with LPS group,serum levels of TNF-α and IL-6 significantly decreased ( P <0. 05 ) , whereas IL-10 increased ( P <0. 05) in epinephrine group. (4)Compared with LPS group,BALF levels of TNF-α[(78 ± 9)ng/L vs. (102 ±16)ng/L]andIL-6[(268±42)ng/Lvs.(347±50)ng/L]significantlydepressed(P<0.05),whereas BALFlevelsofIL-10[(210±23)ng/Lvs.(146±34) ng/L]elevated(P <0.05) inepinephrinegroup. Conclusion Epinephrine could reduce the acute lung injury caused by LPS. Its protective effect may be re-lated to decreasing the levels of TNF-α and IL-6,elevating IL-10 level.

Circulating DNA is defined as a kind of extracellular DNA that exists in plasma,cerebrospinal fluid and synovial fluid.The concentration of circulating DNA of cancer patients is significantly higher than that in healthy people.The genetic and epigenetic alterations of circulating cell-free nucleic acids are relevant to cancer development and progression,for example,gene mutation,DNA methylation and microsatellite instability and so on.The quantitative and qualitative detection of circulating DNA shows promising potential value in cancer screening,diagnosis,disease monitoring treatment and prognosis.

Objective:To investigate the effects of CCR3 on Muc5ac in the airway of asthmatic mice.Methods: Fifty clean BALB/c mice were randomly divided into control group,asthmatic group,dexamethasone treatment group,SB328437 treatment group, vehicle-control group.Total cells and differential inflammatory cells were counted in BALF, the levels of IL-4 and TNF-αwere determined by ELISA,lung tissue HE staining the expressions of Mucin5ac(Muc5ac) and CCR3 in lung tissue were detected by immu-neohistochemical staining and RT-PCR.Results:The total cells,eosinophil,monocytes and lymphocyte cells in BALF,the levels of IL-4,TNF-αin BALF ,the goblet cell of airway wall,the expression of Muc5ac and CCR3 positive staining IOD in the airway and the expression of Muc5ac and CCR3 mRNA lung tissue obviously decreased in SB328437 treatment group and DEX treatment group which compared with asthmatic mice model group ( P<0.05 ) .Conclusion: SB328437 can inhibit the expression of CCR3 in pulmonary tissue,furtherly inhibit the expression and secretion of Muc5ac and control the airway inflammation.

OBJECTIVE To investigate the pathogens in the early period after heart transplantation and analyze their drug-resistance.METHODS The pathogens in the early period after heart transplantation were identified and their drug-resistance was analyzed.RESULTS From all of the 121 pathogens,the rate of G-bacilli was 73.6%,the rate of G+ cocci was 17.4%and the rate of fungi was 9.1%;G-bacilli mainly consisted of Klebsiella pneumoniae,Enterobacter cloacae,Acinetobacter baumannii,Pseudomonas aeruginosa and Escherichia coli.G-bacilli showed higher sensitive rates to sulbactam/cefoperazone,cefepime,piperacillin/tazobactam and ceftazidime than to cefotaxime.All G-bacilli showed sensitive to imipenem except Pseudomonas aeruginosa.G+ cocci mainly consisted of negative coagulase Staphylococcus,Staphylococcus aureus and Enterococcus.Fungi mainly consisted of Candida,and they were sensitive to fluconazole,itraconazole and amphotericin B.CONCLUSIONS To observe the pathogens in the early period after heart transplantation and analyze their drug-resistance are important to control and prevent the infection efficiently for the heart transplantation recipients.

Objective To explore the protective role of the receptor tyrosine kinase inhibitor in chondrocyte in vitro.MethodsReverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry (ICC) and toluidine blue staining were used to understand the changes of phenotype expression of collagenⅠ, Ⅱ and proteoglycan in chondrocyte in vitro. Results Genistein of 20 μg/mL and 25 μg/mL concentrations decreased the expression of collagen Ⅰ and increased the expression of collagen Ⅱ and proteoglycan (P< 0. 05). Conclusions This study showes that genistein can effectively inhibit the expression of collagen Ⅰ above certain concentration, and it can improve the expression of collagen Ⅱ and increase the synthesis of proteoglycan.