OBJECTIVE To investigate the pathogens and their drug-resistance in Fuwai Cardiovascular Hospital and provide antibiotics use suggestion for clinical treatment.METHODS The pathogens were identified by VITED 32 and analyzed by WHONET 5.4 RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were highly sensitive to imipenem and meropenem except Pseudomonas aeruginosa;E.faecalis was much more sensitive to penicillin and gentamicin than E.faecium.Most coagulase-negative Staphylococcus(CNS) were resistant to oxacillin and showed low susceptibility rates to most antibiotics.No Gram-positive cooci were found to be resistant to vancomycin and teicoplanin.CONCLUSIONS To investigate the pathogens and their drug resistance is very important to prevent and control nosocomical infections.

OBJECTIVE To investigate the major pathogens and their antibiotics resistance in surgery wards in Fuwai Cardiovascular Hospital.METHODS The pathogens were classified and their antibiotics resistance was analyzed.RESULTS The pathogens mainly consisted of Gram-negative bacilli,which were sensitive to cefoperazone/sulbactam,pipercillin/tazobactam and amikacin with the sensitive rate of 62.7-97.6%.All Gram negative bacilli were sensitive to imipenem except Pseudomonas aeruginosa,with the sensitive rate of 95.2%~100%.In the Gram-positive bacteria,the susceptibility rates of Enterococcus faecium were lower than E.faecalis.Coagulase negative Staphylococcus and Staphylococcus aureus were mainly resistance to oxacillin and most other antibiotics,all Gram positive bacteria were sensitive to vancomycin.CONCLUSIONS To know the major pathogens and their susceptibility rates can prevent and control nosocomial infections effectively.

Objective To detect the value of qualitative and orientating diagnosis in the degeneration stage of cerebral cysticercosis with Gd-DTPA enhanced MR. Methods Sixty-nine cases of cerebral cysticercosis were diagnosed by enhanced MR as degeneration stage, and confirmed by immunological examination and/or by surgery. MR plain scanning was conducted for the same cases. Results The plain scanning showed single or multiple lesions with long T1 and long T2 signals, and the enhanced scanning showed nodular or annular lesions. The diameter of the lesion after enhanced scan was not more than 22mm with an average value of 8.1mm. Some cases showed single lesion on plain scanning but showed multiple lesions after enhanced scan. Conclusion The enhanced MR shows more typical features of the degeneration stage cerebral cysticercosis. It can define the number, position and range of the lesions, and can improve the accuracy of differential diagnosis, and therefore be of importance in formulating treatment scheme and prognosis.

OBJECTIVE:To give some information for the strategy of joint technical innovation in our country.METHODS:To analyse the real situation of joint technical innovation in China and to summarize the experiences of international pharmaceutical corporations.RESULTS & CONCLUSION:Joint technical innovation is the general trend of development,we should take measures according to the existing problems in Chinese pharmaceutical industry.

BACKGROUND:Intrauterine device made of bare copper can release a great amount of oxides that are easy to cause bleeding and pain in women. Nano copper/low-density polyethylene intrauterine device can wel solve the above drawbacks, which is conducive to the maintenance of the reproductive health of women of childbearing age. OBJECTIVE:To explore the effect of nano copper/low-density polyethylene intrauterine device on post-implantation uterine bleeding and pain. METHODS:A total of 98 voluntary women asking for intrauterine device insertion were selected and randomized into control group (n=49) with 220C (TCu220C) T type copper intrauterine device and observation group (n=49) with nano copper/low-density polyethylene intrauterine device. Al the women were folowed up for 12 months, and incidence of adverse events, including uterine bleeding and pain, was observed and compared between the two groups. RESULTS AND CONCLUSION: During the folow-up, there were one case of uterine bleeding and two cases of pain in the observation group and the incidence of adverse events was 6%; in the control group, there were five cases of uterine bleeding and eight cases of pain, and the incidence of adverse events was 27%. There was a significant difference in the incidence of adverse events between the two groups (P< 0.05). These findings indicate that the nano copper/low-density polyethylene intrauterine device can effectively reduce the occurrence of uterine bleeding and pain after implantation.

Objective To study the relation between IL-8 gene-251A/T polymorphism and delayed Alzheimer's disease (AD)in Xinjiang Han people .Methods Eighty delayed AD patients served as a delayed AD group and 80 subjects undergoing physical examination served as a control group in this study .Distribution of IL-8 gene-251A/T polymorphism in two groups was detected by PCR-RFLP .Results The distribution of genotypes and alleles was different in two groups (P=0 .023 , P= 0 .010) .Further stratified analysis showed that the frequency of A allele was significantly higher in delayed AD group than in control group (OR= 1 .851 ,95% CI:1 .159 -2 .957 ,P=0.010) ,indicating that A allele is the risk factor for delayed AD .The frequency of AA genotype was significantly higher in delayed AD group than in control group (OR=3 .370 ,95% CI:1 .143-9.939 ,P=0 .023) ,indicating that the risk of delayed AD was 3 .370-fold higher in subjects with AA genotype than in those with TT genotype .The AT genotype was not related with the risk of delayed AD (OR=1 .944 ,95% CI:0 .994-3 .803 ,P=0 .051) .Conclusion IL-8 gene-251A/T pol-ymorphism is related with the risk of delayed AD .

Objective To observe and analyze the change state of serum inflammatory mediators of patients with secondary septic shock after chemotherapy for acute leukemia .Methods 56 patients with secondary septic shock after chemotherapy for acute leukemiawere were selected as the group A,56 patients without secondary septic shock after chemotherapy for acute leukemia were selected as the group B ,56 healthy persons of the same age were selected as the group C,then the serum inflammatory mediators levels of three groups were detected and compared,and the serum indexes of group A with different severity degree of shock were compared, then the detection results of group A and group B with different types ( lymphocytic leukemia and nonlymphocytic leukemia)were compared.Results The serum inflammatory mediators levels, including TNF-α, IL-4, IL-6, IL-10 and other inflammatory factors, including PGE2 , sICAM-1, sVCAM-1 and sCD44, there were group A >group B >group C, severe shock >moderate shock>mild shock ( all P<0.05 ) .The above serum inflammatory mediators and other inflammatory factors levels of different types in group A were higher than those in group B, and the above mediators in lymphocytic leukemia of group A and group B were higher than those in nonlymphocytic leukemia (all P<0.05).Conclusion The serum inflammatory mediators of patients with secondary septic shock after chemotherapy for acute leukemia show higher expression state,and the influence of shock degree and leukemia types for the expression are great.

[Objective]To study the effect of sixpetal clematis root injection(WLX)on histology,collagen phenotype and ultramicrostructurc in cartilage of osteoarthritis animal model.[Method]OA model animals were randomly divided into 3 groups:group S,group D and group K.Animals in group S received WLX and animals in group D received sterilized normal saline intramuscularly,group K was control and received nothing.After the animals were killed,cartilage specimen were obtained and sections were made and stained with HE/SOFG for Mankin score;the expression level of collagen Ⅰ and Ⅱ in matrix was tested by immunohistochemistry;change of ultramicrostructure of cartilage was observed with transmission electron microscope.[Result]Mankin score in group S was better than that in group D and K at each test period;result of IHC shows that positive stain area of collagen Ⅰ was lower in group S than that in group D and K,and positive stain area of collagen Ⅱ was higher than that in group D and K at each test period;cartilage impairment degree in group S was lower than in group D and K,and impairment manifestation in group D and K was similar on the whole.[Conclusion]sixpetal clematis root injection may protect articular cartilage by maintaining and promoting the chondrocyte synthesizing proteoglycan and type Ⅱ collagen.

Objective:To investigate the effects of CCR3 on Muc5ac in the airway of asthmatic mice.Methods: Fifty clean BALB/c mice were randomly divided into control group,asthmatic group,dexamethasone treatment group,SB328437 treatment group, vehicle-control group.Total cells and differential inflammatory cells were counted in BALF, the levels of IL-4 and TNF-αwere determined by ELISA,lung tissue HE staining the expressions of Mucin5ac(Muc5ac) and CCR3 in lung tissue were detected by immu-neohistochemical staining and RT-PCR.Results:The total cells,eosinophil,monocytes and lymphocyte cells in BALF,the levels of IL-4,TNF-αin BALF ,the goblet cell of airway wall,the expression of Muc5ac and CCR3 positive staining IOD in the airway and the expression of Muc5ac and CCR3 mRNA lung tissue obviously decreased in SB328437 treatment group and DEX treatment group which compared with asthmatic mice model group ( P<0.05 ) .Conclusion: SB328437 can inhibit the expression of CCR3 in pulmonary tissue,furtherly inhibit the expression and secretion of Muc5ac and control the airway inflammation.

BACKGROUND:Hemostatic dressing can directly contact with the body tissues on the wound surface. The biocompatibility is one of the important indicators of evaluating the advantages and disadvantages of dressing. The hemostatic dressing prepared with calcium alginate as raw material has become a research focus owing to its low cost and good compatibility. OBJECTIVE:To observe the cytotoxicity of calcium alginate hemostatic dressings and to compare the cytotoxicity between calcium alginate hemostatic dressing and gelatin hemostatic sponge, absorbing cotton, ordinary gauze. METHEDS:Leaching solution method: the DMEM high glucose culture solution was taken as the leaching medium. The calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze leaching solution were respectively prepared. Five concentration gradients of 100%, 75%, 50%, 25%, 50% were set. The fibroblast cels of L-929 mouse were cultured for 24 hours with the above material leaching solution. The volume fraction of 10% DMEM high glucose culture medium was taken as control group, and DMEM high glucose culture medium containing 5% DMSO was taken as positive control group to observe the cel proliferation and morphological changes. Direct contact method: The fibroblast cels of L-929 mouse were respectively seeded in calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze and cultured for 24 hours. The changes in cel morphology were observed.
RESULTS AND CONCLUSION: Leaching solution method: The cytotoxicity of alginate fiber hemostatic dressing, gauze, absorbing cotton leaching solution with different concentration gradients was grade 1, which was in line with GB/T16886/ ISO10993 biological evaluation standard of medical apparatus and instruments. The cytotoxicity of 100%, 75% gelatin hemostatic sponge extract solution was grade 3, causing severe inhibition of cel proliferation. Direct contact method: The cytotoxicity of gauze and alginate fiber hemostatic dressing was grade 1, absorbing cotton was grade 2, gelatin hemostatic sponge was grade 3. These results demonstrate that calcium alginate hemostatic dressing has no cytotoxicity.