Objective To reduce the probability of adverse transfusion reaction and ensure the safety of transfusion according review the clinical cases.Methods The record of adverse transfusion reaction was retropectivly analyzed.Results 81 cases were occured adverse reactions caused by transfusion in 38 490 cases of our hospital, with the rate of 0.21%,including 52 cases of anaphylactic transfusion reaction and 29 cases of febrile non -hemolytic transfusion reaction.There wasn't statistically significant difference among of those blood components(χ2 =3.849,P >0.05).Conclusion We should renovate the scientific and reasonable concept of blood transfusion,encourage autolo-gous transfusion,strictly abide by the lows and regulations realated to transfusion,the probability of adverse transfusion reaction would be reduced.

Objective Compared study on microcolumn gel test (MGT)and tube anti -globulin test (TAT) in the detection of pregnant women IgG anti A (B)in the application of antibody and evaluate the application value of MGT in the prediction of HDN.Methods Choosed blood samples of 443 cases of O blood type pregnant women whose husband were not O blood type as the research object.Every specimen were tested by MGT method and TAT method,and the data were treated statistically.Results The positive rate of MGT method and TAT method were:30% and 12.5% which had statistical significance(χ2 =15.95,P <0.05).The difference was significant in positive cases titer distribution(t =15.13,P <0.01).Conclusion The micro column gel method is rapid,simple,sensitive and repeatable compared of tube anti -globulin test (TAT).

Objective To compare and anlyze the applications of microcolumn gel test (MGT)and the tube anti -globulin test (TAT)for detection of three hemolytic tests.Methods Microcolumn gel test and tube anti -globulin test were used in three tests with a total of 160 cases of blood speciments of newborn who had blood group in-compatibility with their mothers:direct anti -globulin test,free test and elution test,then the data were analyzed statis-tically.Results The positive rates of direct anti -globulin test of MGT and TAT were 30.0% and 12.5%(χ2 =14.64,P <0.05);The positive rates of free test of MGT and TAT were 47.5% and 25.0%(χ2 =17.53,P <0.05), respectively;The positive rates of elution test of MGT and TAT were 62.5% and 30.0%(χ2 =33.99,P <0.05). There was statistically significant difference between two methods in three tests.Conclusion MGT has the advantages of quickness,simplicity,high sensitivity,convenience,needs less sample,high repeatability,direct judging of the re-sults,which is better than TAT in three hemolytic tests.

Background and purpose:Tumor microenvironment plays an important role in the introduction of foreign factors that mediate tumor acquired resistance. The antitumor effects of many chemotherapeutic agents depend on the level of oxygen pressure (pO2) in tumor microenvironment. This study aimed to evaluate electron paramagnetic reso-nance (EPR)-based monitoring on an oxygen-enriched tumor microenvironment to increase chemotherapeutic sensitivity. Methods:MCF-7 cells were used to establish human breast cancer in nude mice. EPR was used to directly measure pO2 levelin vivo. Tumor tissues were collected, and mitochondrial activity was assayed on the basis of the kinetics of enzyme-catalyzed reactions. A laser Doppler monitor was used to detect regional blood flow. Tumor apoptotic rate was analyzed by flow cytometry.Results:The tumor volume decreased more evidently in the chemotherapy group with oxy-gen-enriched environment than that in the conventional chemotherapy group after the treatment was administered (P<0.01). After chemotherapy was completed, the apoptotic rate of tumor cells was significantly higher in the chemotherapy group with oxygen-enriched environment than that in the conventional chemotherapy group (P<0.001). This study examined the mechanism ofpO2 changes in tumor microenvironment: This was related to the change of the balance between the oxygen consumption and the regional blood flow in the tumor tissues after chemotherapy.Conclusion:Based on the characteristics ofpO2 changes in the tumor microenvironment after chemotherapy was completed, the selection of chemotherapy mode forthe treatment inpO2 peak time window improves the sensitivity of chemotherapy, which provides a new idea for individual-ized chemotherapy in clinical applications.

Objective:To analyze the clinical significance of thromboelastography in the treatment of postpartum hemorrhage(PPH).Methods:From May 2014 to March 2020, 62 patients with PPH in the People's Hospital of Gaomi were selected and divided into control group and observation group according to the random digital table method, with 31 cases in each group.The control group was treated with blood transfusion under the guidance of routine coagulation test, the observation group was treated with blood transfusion under the guidance of thromboelastogram.The number and volume of platelet, plasma and cryoprecipitation were compared between the two groups.Results:There were 9 cases of platelet transfusion, 19 cases of plasma transfusion and 11 cases of cryoprecipitation transfusion in the observation group, and there were 18 cases, 27 cases and 19 cases in the control group, respectively.The number of platelet, plasma and cryoprecipitation transfusion in the observation group was lower than that in the control group, the differences were statistically significant(χ 2=5.314, 5.391, 4.133, all P<0.05). The platelet transfusion[(0.80±0.25)u], plasma [(258±60)mL] and cryoprecipitation transfusion [(9.2±2.5)u] in the observation group were lower than those in the control group ( t=5.769, 8.669, 8.802, all P<0.05). Conclusion:For women with PPH, the use of TEG for transfusion therapy is better, and the test is worthy of promoting in the clinic.