Objective:To explore the value of metagenomic next-generation sequencing (mNGS) in the pathogen diagnosis of liver abscess.Methods:A perspective study was performed in 35 hospitalized patients with liver abscess in Department of Emergency Medicine, Zhongshan Hospital, Fudan University from February 2020 to April 2021. Blood samples and abscess drainage fluid samples were detected by routine microbial culture and mNGS. Patients were divided into two groups according to whether they had septic shock or not. SPSS 25.0 was used for statistical analysis.Results:The overall positive rate of mNGS in blood samples and drainage fluid samples was significantly higher than that of routine microbial culture methods (blood: 67.6% vs. 15.2%, P<0.05; Drainage fluid: 100% vs. 55.2%, P<0.05). In 35 patients with liver abscess, 71.4% of the pathogens were Klebsiella pneumoniae. The sequence number of pathogenic pathogens detected by mNGS in abscess drainage fluid samples of patients in the shock group was significantly higher than that in the non-shock group ( P<0.05). Conclusions:The mNGS can quickly and accurately detect the pathogen of liver abscess, which can provide important etiological diagnostic for clinical treatment.

Objective To evaluate the clinical efficacy of qi-replenishing,phlegm-resolving and stasis-diapelling formula,a Chinese compound medicine,on the patients of stable stage with chronic obstructive pulmonary disease (COPD).Methods Altogether 51 COPD patients with TCM syndrome of deficiency of lung qi,spleen qi,kidney qi and phlegm-stasis obstruction were randomly divided into Chinese medi-cation group (n =26)and control group (n =25).The patients of the control group were treated with only routine Western medication,while those of the Chinese medication group were treated with the rou-tine Western medication combined with TCMformula for consecutive four months.Results There were significant differences of forced expiratory volume in one second (FEV1 ),changes of symptom scores and COPD assessment test (CAT)scores between after treatment and before treatment in Chinese medi-cation group(P <0.05).Compared with control group,all the indexes mentioned above showed statisti-cal differences in Chinese medication group(P <0.05).Conclusion The clinical efficacy and safety of Chinese formula were proved by the means of comparison of FEV1,symptom scores and CAT scores be-tween Chinese medication group and control group.

To assess the normal value of left ventricular twist (LVtw) and examine the changeswith normal aging by 2-dimensional ultrasound speckle-tracking imaging (STI), 121 healthy volunteers were divided into three age groups: a youth group (19-45 y old), a middle-age group (46-64 y old ) and an old-age group (≥65 y old). Basal and apical short-axis images of left ventricular were ac- quired to analyse LV rotation (LVrot) and LVrot velocity. LVtw and LVtw velocity was defined as apical LVrot and LVrot velocity relative to the base. Peak twist (Ptw), twist at aortic valve closure (AVCtw), twist at mitral valve opening (MVOtw), untwisting rate (UntwR), half time of untwisting (HTU), peak twist velocity (PTV), time to peak twist velocity (TPTV), peak untwisting velocity (PUV), time to peak untwisting velocity (TPUV) were separately measured. The results showed that the normal LV performs a wringing motion with a clockwise rotation at the base and a counterclock- wise rotation at the apex (as seen from the apex). The LVtw velocity showed a systolic counterclock- wise twist followed by a diastolic clockwise twist. Peak twist develops near the end of systole (96%±4.2% of systole). With aging, Ptw, AVCtw, MVOtw, HTU and PUV increased significantly (P<0.05) and UntwR decreased significantly (P<0.05). However, no significant differences in TPUV, PTV and TPTV were noted among the 3 groups (P0.05). It is concluded that LV twist can be meas- ured non-invasively by 2-dimensional ultrasound STI imaging. The age-related changes of LVtw should be fully taken into consideration in the assessment of LV function.